Team:NUDT CHINA/Notebook

Development of A Novel

Blood-MicroRNA Handy Detection System with CRISPR

Chuanyang Liu, Changsong Gao, Chushu Zhu and Qijie Xu completed most of the experiments.

Fig.1 OD600 reference point
scx

Data

Project

6.15-7.4

Finish the culture of A549 cell and optimization of culture condition with Chuanyang Liu

7.5-8.13

Work on Western Blot assay for target gene expression with Chuanyang Liu

8.14-8.26

Do Western Blot assay EMT markers with Chuanyang Liu

8.27-9.22

Finish the Cell proliferation and migration assay of miR-214 Locker group with Chuanyang Liu

9.22-10.14

Finish the Cell proliferation and migration assay of miR-654 Locker group with Chuanyang Liu
Fig.1 OD600 reference point

Data

Project

6.15-7.4

Finish the Phosphorylation and annealing of the oligos with Qijie Xu

7.5-8.13

Work on miRNA locker assembly with Qijie Xu

8.14-8.26

Finish the Asymmetric PCR to generate ssDNAs from dsDNAs with Qijie Xu

8.27-9.22

Construct parts with Qijie Xu

9.22-10.14

Work on the experimental validation of parts with Qijie Xu
Fig.1 OD600 reference point

Data

Project

6.15-7.4

Finish the Phosphorylation and annealing of the oligos with Chushu Zhu

7.5-8.13

Work on miRNA locker assembly with Chushu Zhu

8.14-8.26

Finish the Asymmetric PCR to generate ssDNAs from dsDNAs with Chushu Zhu

8.27-9.22

Construct parts with Chushu Zhu

9.22-10.14

Work on the experimental validation of parts with Chushu Zhu