Team:NUDT CHINA/Notebook
Development of A Novel
Blood-MicroRNA Handy Detection System with CRISPR
Chuanyang Liu, Changsong Gao, Chushu Zhu and Qijie Xu completed most of the experiments.
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Data |
Project |
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6.15-7.3 |
Construct plasmid expressing GFPnano-IgG1Fc and Trim21 with Tianyi Zhang. |
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7.4-8.3 |
Work on the optimization of transfection and fluorescence-detecting conditions in Hela and A549 cells with Tianyi Zhang. |
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8.4-8.15 |
Work on Western Blot assay for target gene (GFP) expression and fluorescence detection with Tianyi Zhang. |
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8.16-8.28 |
Work on repeated validation of GFP interfering efficiency with Tianyi Zhang. |
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8.29-9.22 |
Work on transfection optimization, EMT-related Markers detection of ErbB3-interfered cells with Tianyi Zhang. |
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9.22-10.10 |
Work on the proliferation and migration assay of cells with ErbB3 interfered with Tianyi Zhang. |
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Data |
Project |
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6.15-7.4 |
Finish the culture of A549 cell and optimization of culture condition with Chuanyang Liu |
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7.5-8.13 |
Work on Western Blot assay for target gene expression with Chuanyang Liu |
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8.14-8.26 |
Do Western Blot assay EMT markers with Chuanyang Liu |
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8.27-9.22 |
Finish the Cell proliferation and migration assay of miR-214 Locker group with Chuanyang Liu |
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9.22-10.14 |
Finish the Cell proliferation and migration assay of miR-654 Locker group with Chuanyang Liu |
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Data |
Project |
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6.15-7.4 |
Finish the culture of A549 cell and optimization of culture condition with Chuanyang Liu |
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7.5-8.13 |
Work on Western Blot assay for target gene expression with Chuanyang Liu |
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8.14-8.26 |
Do Western Blot assay EMT markers with Chuanyang Liu |
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8.27-9.22 |
Finish the Cell proliferation and migration assay of miR-214 Locker group with Chuanyang Liu |
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9.22-10.14 |
Finish the Cell proliferation and migration assay of miR-654 Locker group with Chuanyang Liu |
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Data |
Project |
|
6.15-7.4 |
Finish the culture of A549 cell and optimization of culture condition with Chuanyang Liu |
|
7.5-8.13 |
Work on Western Blot assay for target gene expression with Chuanyang Liu |
|
8.14-8.26 |
Do Western Blot assay EMT markers with Chuanyang Liu |
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8.27-9.22 |
Finish the Cell proliferation and migration assay of miR-214 Locker group with Chuanyang Liu |
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9.22-10.14 |
Finish the Cell proliferation and migration assay of miR-654 Locker group with Chuanyang Liu |