Team:Austin UTexas/Results/Assemblies

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Assembly Plasmids

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Part Plasmids Assembly Plasmids Electroporations Conjugations

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Using five single parts and one bridge-type part, full plasmids are assembled in a single BsaI reaction. An assembled plasmid then contains an antibiotic resistance gene, origin of replication, origin of transfer, barcode sequence, and a part 1-5 bridge, which contains a colored reporter protein coding sequence. The same promoters, terminators, and origins of transfer are used within all assemblies. This is not true for the barcode, and reporter protein sequences, as they indicated which origin of replication the plasmid contains. This design eliminates as many variables as possible, allowing the origin of replication to be the single largest factor determining whether or not the bacteria sustain and express the plasmid.