Team:Austin UTexas/Parts
Parts
BBa_K2657001
When observing the parts registry, we found that no tetracycline resistance gene had Phytobrick compatibility. Using primers, we added proper MoClo standard overhangs to each part as well as providing it the proper restriction sites — BsaI and BsmBI sites. The PCR products containing these overhangs were incorporated into the Universal Acceptor backbone, BBa_P10500, which is a modified pSB1C3 backbone. Link to Part Page
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BBa_K2657002
At the same time while looking over the parts registry, we found no origin of transfer with Phytobrick overhangs. Using the same techniques that were used to make the TetR Phytobrick, we created a the Rp4 Phytobrick, which is a level 0 OriT Phytobrick.Link to Part Page