Plasmids used during the characterization of our biobrick
Overview of our experimental design to archive the results shown below
Figure x. A simple explaination on how we archived our experimental results. The plasmids shown is the same as before, where A can be either of mNG-Aß1-42, EGFP-Aß1-42, a-synuclein-EGFP and Tau0N4R-EGFP, B is always pGroE7 and C is pSB4A5-GroES. The concentrations used for inducing the chaperone plasmids was 0.25 mg/ml L-arabinose, 200 ng/ml tetracycline. For the substrate plasmids we used 0.5 mM IPTG, and the substrate was induced 30 mins after the chaperone plasmids.
Results for the mNG-Aß1-42 substrate protein
Results for the EGFP-Aß1-42 substrate protein
Results for the a-synuclein-EGFP substrate protein
