Plasmids used during the characterization of our biobrick
Figure x. Plasmids used in our experiments. pSub. can be either of mNG-Aß1-42, EGFP-Aß1-42, a-synuclein-EGFP and Tau0N4R-EGFP. As seen in the next figure, four combinations were introduced into E.coli (BL21). Substrate, substrate and GroES, substrate and GroES and GroE, substrate and GroE.
Overview of our experimental design to archive the results shown below
Figure x. A simple explaination on how we archived our experimental results. The plasmids shown is the same as before, where A can be either of mNG-Aß1-42, EGFP-Aß1-42, a-synuclein-EGFP and Tau0N4R-EGFP, B is always pGroE7 and C is pSB4A5-GroES. The concentrations used for inducing the chaperone plasmids was 0.25 mg/ml L-arabinose, 200 ng/ml tetracycline. For the substrate plasmids we used 0.5 mM IPTG, and the substrate was induced 30 mins after the chaperone plasmids.
Results for the mNG-Aß1-42 substrate protein
Results for the EGFP-Aß1-42 substrate protein
Results for the a-synuclein-EGFP substrate protein
