Team:William and Mary/3GProtocols
3G Assembly Protocols
Overview
3G assembly is a new and cutting edge hybrid method of DNA assembly first described in 2018 by A. D. Halleran, A. Swaminathan and R. M, Murray [1] combines Golden Gate and Gibson to allow for modular assembly of multi-part circuits in a single day. In this method circuits are composed of modular transcriptional units constructed using Golden Gate Assembly. These transcriptional units are amplified with PCR, purified via gel extraction, then combined into a circuit using Gibson Assembly.
References
[1] Single Day Construction of Multigene Circuits with 3G Assembly
Andrew D. Halleran, Anandh Swaminathan, and Richard M. Murray. ACS Synthetic Biology 2018 7 (5), 1477-1480. DOI: 10.1021/acssynbio.8b00060