Team:William and Mary/Measurement
Measurement
Overview
This year team William and Mary's project focused on measuring the dynamical outputs of our decoding circuit. In the process we performed time series measurements, focusing on scalability and parameter testing. Although single cell measurements using flow cytometry are our preferred way to obtain data, for much of our project we were required to test for qualitative circuit behavior using a plate reader due to the number of constructs we had to test. However
Figure 2: Relative fluorescence (fluorescence/max) measurements of the temperature activatable circuit BBa_K2680051 when grown at 30C and then activated by exposure to 37C for the entirety of the experiment (A) or transiently (B). Dots represent the geometric mean of 3 (B) or 6 (A) distinct biological replicates (colonies) and the blue shaded region represents one geometric standard deviation above and below the mean. The grey shaded region in (B) represents the period in which the temperature was 37C. Normalized fluorescence (relative to max) was calculated by dividing each colony relative to it's maximal expression.