Team:UMaryland/PETaseIntro

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History
Past iGEM Teams involved with PET
Listed here are past iGEM teams that have worked on projects on or related to PET degredation.
2012
BAU-Indonesia
  • Isolation of cutinase gene from nature with primers

TU Darmstadt
  • Surface display of cutinase on E. coli
  • Attempted TPA transport into E. coli, further research required
  • Expressed all TPH enzymes, did not attempt to measure activity
  • Confirmed anaerobic conversion of PCA via AroY and XylE enzymes

UC Davis
  • Confirmed cutinase activity using PNPB esterase assay
  • Engineered E. coli ethylene glycol metabolism with directed evolution

2013
Imperial College
  • Produced P3HB bioplastic from mixed waste containing at least some PET

2014
METU Turkey
  • Reduced catechol to pyruvate

ITB Indonesia
  • LC cutinatse activity confimed with SEM, PNPB

2015
Pasteur Paris
  • PNPB assay to confirm activity of esterase EST13
  • Fluorescent detection of TPA can not be accomplished when in LB broth

2016
ASIJ Tokyo
  • Attempt at detecting PET degradation by mass change failed

AUC Turkey
  • Withdrawn

Baltimore BioCrew
  • Planned to weigh PET degradation, no results

BGU Israel
  • PNPB and EM to confirm LC cutinase activity
  • P. putida can grow on PCA as sole carbon source, but not TPA
  • E. coli expressing LC-cutinase with pelB leader sequence grew on M9 plates with PET as sole carbon source. Expected to be due to consumption of ethylene glycol from PET degradation
  • Unable to determine enzyme efficiency based on growth due to heterogeneity in PET distribution
  • Measured fluorescence of TPA on plates, unable to quantify LC cutinase activity

Harvard BioDesign
  • Petase function confirmed with PNPB
  • Bacteria produced electric current when supplied with unspecified quantity of TPA

Tianjin
  • EM confirmation of PETase activity of PET film degradation
  • Multispectral scanning quantified PETase products for cell free system

TJUSLS China
  • HPLC detection of MHET to confirm PETase activity in varying conditions
  • Surface display of PETase in E. coli

UESTC-China
  • SEM and PNPB to confirm PETase activity
  • Possible detection of TPA by UV vis (higher absorbance across spectrum)

UoA New Zealand
  • Assembled PETase part with His tag

2017
Baltimore Bio-Crew
  • Fluorescine diacetate hydrolysis assay to confirm PETase and MHETase hydrolytic activity

BOKU-Vienna
    Discussion of a possible method for directed evolution of PETase by culturing cells on PET film that fluoresces when degraded

ITB Indonesia
  • Successful biofilm formation on PET, but biofilm matrix hampered PETase activity
  • Engineered E. coli ethylene glycol metabolism with directed evolution

2018
Makerere University
  • TBA

OLS Canmore Canada
  • TBA

RHIT
  • TBA

ULaVerne
  • TBA

UMaryland
  • Check out our results on this wiki!

Yale
  • TBA

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