Collaboration
Over the summer our team traveled to the campus of the University of Maryland for the 2018 Mid-Atlantic Meetup. This meet-up was a great opportunity to talk to the other teams from the region and get feedback for our project. Two teams present were particularly interested in the 3G portion of our project: University of Virginia and University of Pittsburgh. We quickly began a correspondence and, to demonstrate the accessibility and ease of 3G assembly we sent them the supplies and instructions to perform a 3G assembly at their own lab. The package consisted of a parts kit, a transcriptional unit assembly protocol, a Gibson protocol and calculator, 100mM of IPTG, and 200ug/ml of ATC. The parts kit included a small subset of our own 3G assembly library, normalized to 30nM, 50nM of UNS adapters, and 10uM of primers for amplification. The parts are listed below.