Team:SHSBNU China/InterLab
InterLab
I. Background
“All of 2018 iGEM teams are invited and encouraged to participate in the Fifth International InterLaboratory Measurement Study in synthetic biology.” Our team took part in this study which is aimed to figure out and correct the sources of systematic variability in synthetic biology measurements. The main task was to quantify expression of GFP in common, comparable or absolute units. In our case, we measured fluorescence using plate reader.
II. Design
Fluorescence is widely used as a proxy for promoter activity by expressing fluorescent proteins such as green fluorescent protein (GFP). Despite this is an indirect measurement, it provides a useful insight into expression levels and has significant advantage that it could be a real-time monitor without disrupting cells.
Fluorescence/OD600 is routinely used to give an adjustment of the relative expression per cell.
III. Material and Methods
a. Plasmids Used
| Negative control | BBa_R0040 | (provided in kit plate) |
| Positive control | BBa_I20270 | (provided in kit plate) |
| Test Device 1 | BBa_J364000 | (provided in kit plate) |
| Test Device 2 | BBa_J364001 | (provided in kit plate) |
| Test Device 3 | BBa_J36400 | (provided in kit plate) |
| Test Device 4 | BBa_J364007 | (provided in kit plate) |
| Test Device 5 | BBa_J364008 | (provided in kit plate) |
| Test Device 6 | BBa_J364009 |
b. Strain used
c. Machines
d. Software
f. Results
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