Team:William and Mary/Notebook
Notebook
Week 1: 180528-180604
- Began cloning 3G parts based off the library sent from the Murray lab
- Researched literature on dual inducible promoters
- Sent out several cloned 3G parts for sequencing
- Planned out several IFFLs to test.
Week 2: 180604-180610: 180528-180604
· Continued cloning 3G parts onto our standard WM Pad backbone
· Redesigned primers for cloning 3G promoter parts, as previous ones were faulty
· Received results from sequencing, confirmed parts
· Beginning to assemble circuits using 3G assembly
· Making inducible reporter construct and an mf-Lon based IFFL
- Continued cloning 3G parts onto our standard WM Pad backbone
- Redesigned primers for cloning 3G promoter parts, as previous ones were faulty
- Received results from sequencing, confirmed parts
- Beginning to assemble circuits using 3G assembly
- Making inducible reporter construct and an mf-Lon based IFFL