Team:SHSBNU China/Experiments
Sample
I. Sample
a. Sample
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Outline
Our system includes two main components as mentioned previously. So our experiments were mainly divided in three periods, verifying both parts individually and verifying Biofilm x Laccase system as a whole.
The first period was to construct our biofilm + Spytag, which would be used for combination later on. This period included creating part BBA_K2684006, csgA – SpyTag and BBA_K2684004, sfGFP – SpyCatcher. As well as improving a previously part, BBa_K1583000, csgA. The biofilm + SpyTag ’s ability of combining protein domains + SpyCatcher was measured and our biofilm with SpyTag system tend to be successful.
The second period is focused to construct and measure the laccase activity of our parts: BBA_K2684000, CotA, BBA_K2684001, Pelb - cotA, BBA_K2684002, PhoA - cotA, BBA_K2684003, OmpA - cotA, BBA_K2684005, cotA - SpyCatcher. The laccase activity of the 5 parts were measured.
The final period is mainly focused on combination of period 1 and 2 which means constructing our Biofilm x Laccase system. Experiments were conducted to measure the laccase activity of the total Biofilm x Laccase system. A significant difference was confirmed between the experiment group and the control group. Thus, our system is successful.
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