Team:William and Mary/Notebook

Notebook

Week 1
  • Began cloning 3G parts based off the library sent from the Murray lab
  • Researched literature on dual inducible promoters
  • Sent out several cloned 3G parts for sequencing
  • Planned out several IFFLs to test.
Week 2
  • Continued cloning 3G parts onto our standard WM Pad backbone
  • Redesigned primers for cloning 3G promoter parts, as previous ones were faulty
  • Received results from sequencing, confirmed parts
  • Beginning to assemble circuits using 3G assembly
  • Making inducible reporter construct and an mf-Lon based IFFL
Week 3
  • Completed cloning the portion of our comprehensive 3G assembly part library needed for our circuits
  • Adding remaining 3G parts from Murray’s lab to our library
  • Working on designing and building a dual inducible promoter and low copy backbone for assembly
  • Confirmed minipreps based on sequencing results
  • Started plate reader for experiments on circuits with IPTG and ATC inducer
  • Started using FACS machine for experiments