Team:SDSZ China/Experiment B

iGem SDSZ_China 2018

ABOUT QUORUM SENSING

Quorum sensing is a strategy developed among bacteria that respond to fluctuation of bacteria density in the environment and alters the expression of certain genes. In natural environments, bacteria can control various physiological activities such as bio-illumination, conjugation and so on.

A bacterial QS system (see fig.1)is mediated by what known as the an autoinducer, or a signalling molecule. In low cell density, the autoinducer secreted by individual cells have extremely low density in extracellular environment and are unable to be recognized by receptors and cause significant functional changes in other cells because of the low rate of diffusion. Yet as cell population continues to grow, more autoinducers are secreted and will eventually reach the threshold density. By which point the density is significant enough to activate receptors and began their impact upon cell functions.

As we have already learnt the mechanisms of the system, it’s parts can be used in genetic engineering and help us sorting out problems such as low production efficiency.

OUR DESIGN

As our bacteria are programmed to be effective chitin processors and chitosan productors in adequate environments, we hope that during the life cycles of cells and growth of the clone, this function can be executed at its best conditions and do not impose negative effects on the bacteria itself.

Development of a clone includes two major phases which correspond to the different parts of a logistic growth curve: A mass-propagating phase and a slower growth phase (as the population approaches k). In an ideal situation, our bacteria should be able to multiply quickly while population is relatively smaller thus reach the second phase in shortest time by which point it is ready to deliver a steady output. To achieve that goal, we need to make sure that when clones are just beginning to develop only the functions necessary to the cell is enabled. In other words, we do not want the cell to continue expressing CDA in low concentration for it will be extremely ineffective and only increases energy consumption without countable output. After the clone is fully developed it is ready to initiate the chitin production functions and—because of massive numbers of cells—generate a considerable output.

Using the QS system here saves a lot of effort on monitoring the growth of the clone—which would be consuming since we are talking about an industrialized process with machines conducting the fermentation process. Instead of manually inducing the expression of CDA genes under appropriate circumstances, we let the bacteria themselves to decide. –An advantage that is only rendered possible when we are using self-regulatory, flexible organisms.