Team:SDSZ China/Experiment A

iGem SDSZ_China 2018

Obtaining Genes

Both fungal and bacterial acetylases have three functional domains- a deacetylase domain, a chitin binding zone, and a low density lipoprotein receptor region. By using the bioinformatics BLAST technique, a sequence highly conserved with known deacetylase domain sequences was searched in the NCBI soft armor gene database.

chitin deacetylase 1 [Penaeus monodon]

1 atggcaagag taagatgcgg tagttctctt gccctccttg gcattgtgct gccgagcggg
   61 tcaagaggca ggcagtttca gacacgaccg aggatggagc gaaccttcaa gagggaactg
121 tgcaaggata agggcgccgg cgagtggttc cgtctcagcc tcggtgactg tcgcgatgtg
181 atccagtgta ccgacgccgg tcttcaggcg ctgcgttgcc cccacggcct ggccttcaac
241 ctggagcagc agacctgcga ctggaaagcc aacgtcaaga actgcgacag gaaggagaag
301 acgaaggtcg tgaagcctct cttcaacacc gtcgagcccc tttgccagga aaaccagttg
361 gcgtgcggtg acggcacctg tctggatagg caggtcttct gtgatggaaa agaagattgt
421 accgatggtt ctgacgagac cgcatgtgac gtgaaaaatg accccaacag cgctcccatc
481 tgcaacaccg aggactgccg cctccccgat tgctactgct acaacgaccc tagcgagatg
541 ccccacaaca tgaagccttc cgaagtgcct cagatggtca ccatcacttt cgacgatgcc
601 atcaacatca acaacatgga cctgtacgag ctcatcttca agcagcgctt caaccccaac
661 ggctgctcca tcaagtccac cttcttcgtt tctcacaaat acaacaacta caccgccact
721 caggagatgc accgtctggg tcacgagatc gctgttcact ccatcaccca cgcaaacaac
781 gagactttct ggtcccacgc aagcgaagac gagtacgagc gcgaaatggg tggtgcccgc
841 gtcatcatcg aacgcttcgc caacatcacc gaccagtcca tcatcggcat gcgtaatccc
901 ttcctccgtg tgggcggcaa cagccagttc aggatgatgg agaagaacac cttcctgtac
961 gactccacca tcactgcccc cctgtcctcc atgcccctgt ggccctacac cctgtactac
   1021 cgcatgcccc acccctgcca tggaaacctc cagaactgcc ccactcgctc cttcgcagtg
   1081 tgggagatgg tcatgaacga gatggaccgt cgcgaggaac ccacttacga ggacggcctc
   1141 cccggatgcc acatggtcga ttcctgcttc gccaccaagc ccgagcccga gcagttctac
   1201 aacttcctgc agaacaactt caaccgccac tacaagtcaa accgcgctcc cttcggtctg
   1261 ttcttccact ctgccttcct gaagaacaac cccgacatcc tggacacctt cctctactgg
   1321 ctggacgaga ccctgaagaa ccagaaggac gtttacttcg tcaccatgac ccaggtcatc
   1381 cagtggatgc aggacccccg ccccgtcggc cagctgaaca actacgaggc ctggaaggag
   1441 aagtgcgtcg tcgacggccc acccttctgc tacggcggca acaactgcga gctggacact
   1501 gacgagctcc ctggccagac cctccacctg tccacctgca tgcggtgccc caacaattat
1561 ccctggacaa gggacccatt gggcgaggga ttcttctaa

PREDICTED: uncharacterized protein LOC108681649 [Hyalella azteca]

1 atgctgatgc atgtagccat catgctcatg ctgctggatc agcacgctca aggttcagag
   61 cggcaccccc ggcagagcag caacgccacc accacagagc agctctgcag aggccgcgac
121 tctgaggagt acttcagact ctctgccggc gacgactgca gagacgttgt tagatgtgac
181 cgcagtggac gctctggccc cagccgcctg gccgcagtgc ggtgccccaa cggcctcgcc
241 ttcgacgtcg acagacaagt ctgtgactgg aagaccaaag tccgcaactg cgacagactt
301 gagagaccgc ggaagattaa gccgctgctg gtgacggagg agccgctgtg ctcggggacg
361 gacctggcgt gcggcagcgg cgtgtgtgtc gccaaggagc tcttctgtga tggcaaacct
421 gactgtgatg acggttccga cgagaacacg tgtggtgtgg aagaagatcc aaaccgagcc
481 caggtgtgcg acaaaagtca atgcgttttg ccagaatgtt tctgttccgt ggacggcact
541 agaattcctg gtgatatcaa cccaaaacaa acaccgcaaa tgatcaccat cacattttca
601 ggagcaatca accttgataa tgtggatttg tacgatgaca tcttcaacgg ggaaagaaag
661 aatcccaacg gttgtcaagt gaaagcaacg ttcttcacgt cgcacaagta caccaattac
721 tccgcagttc aagaactaca caggaaaggc catgagatcg ctgtgttctc gatctcgaac
781 aaagaaagtc gagactattg gtcccatgga acatacgacg actggcttgc tgaaatggct
841 ggggccagac tcatcgtcga acgtttcgcc aatatcacag ataattccat cgtcgggtta
901 cgagcgccct acctcagggt tggaggaaac gctcagttcg acatgatgaa cgatcagttc
961 ttcttctacg acgcttcgat tacagcgcca ttggggaaac ttccgctatg gccgtacaca
   1021 ttgtacttca ggatgcctca caaatgtcac gggaacggcc aaaattgtcc atcacgctcc
   1081 caccccgtct gggaaatggt catgaacgaa atggacagaa gagacgaccc agagtttgac
   1141 gaaggactat ctgggtgtca ttacgtcgac tcctgcacca acatccggac tccaaagcaa
   1201 tttgcccatt tcctcgagca caacttcaga cgacactaca acacaaaccg tgcaccactc
   1261 ggcttgcatt tccacgcttc ttggctaaaa ggaaacaaaa attttaaaaa ggaactcatc
   1321 aatttcattc agtcaaaatc gggcaaccct gacgtgtatt tcgtgaccat gcttcaagtg
   1381 attcaatgga tgcaagcacc gaccgaagtg gcaggacttc gcgatttccc agaatggaag
   1441 gagaaatgcg acgtgcaagg tctgccattt tgctcattac caaacacttg cccggtgagg
   1501 acacgagaga ttcctgacga gactttgagt ttgttcacct gcatggactg cccccgcaac
   1561 tacccctggc tgctcgaccc taccggggat ggcgtggaaa ttatataa