During PixCell project we discovered that conventional DNA assembly methods not always adapt to rapid troubleshooting of some construct parts. As our initial construct was open for improvement to improve our model we decided to create a library of transcription factors and promoters and try creating new constructs with them. So, we started looking for a more modular method that would allow us to easily vary 1 or 2 components in a construct keeping everything else the same in order to build our library. We found that BASIC was the perfect technology for this.
BASIC Method