Team:Waterloo/Meet the Microbes
Meet the Microbes
Empty JT2
Strain
Escherichia coli JT2 SKA974
This strain has been engineered for optogenetic control using CcaS/R. Its genomic MetE gene is under control of the CcaS/R promoter. It also has a Kanamycin resistance gene in its genome.
Plasmids
- None
Antibiotic Resistance(s)
- Kanamycin
Fluorescent Marker
- None
Can produce methionine?
- No
Which experiments was I involved in?
- Is methionine shared between cells?
- Biobrick BBa_K2573000 characterization
JT2 - CcaS/R
Strain
- Escherichia coli JT2 SKA974 This strain has been engineered for optogenetic control using CcaS/R. Its genomic MetE gene is under control of the CcaS/R promoter. It also has a Kanamycin resistance gene in its genome.
Plasmids
- pSR43.6r (CcaS, its photoreceptor, p15A origin of replication)
- pSR58.6 (CcaR, sfGFP under the CcaR promoter, ColeI origin of replication)
- Note that we made 2 versions of this strain. One contains both these plasmids, unaltered, and was used for characterization experiments. We cut GFP out of pSR58.6 for the second version of this strain, and used it for non-characterization experiments.
Antibiotic Resistance(s)
- Kanamycin
- Spectinomycin
- Chloramphenicol
Fluorescent Marker
- GFP in the characterization version of this strain, only expressed when CcaS/R is activated.
Can produce methionine?
- Yes, but only when CcaS/R is activated.
Which experiments was I involved in?
- Is methionine shared between cells?
- What is the metabolic load of (over)expressing MetE?
- Can we control growth with red and green light?
Empty DH5alpha
Strain
- Escherichia coli DH5alpha
Plasmids
- None
Antibiotic Resistance(s)
- None
Fluorescent Marker
- None
Can produce methionine?
- Yes, with my wild type genomic MetE gene.
Which experiments was I involved in?
- Can we reliably measure 2 populations in co-culture?
DH5alpha - GFP
Strain
- Escherichia coli DH5alpha
Plasmids
- BBa_E0040 in pSB1C3 (GFP biobrick, ColeI origin of replication)
- a plasmid we constructed that has Spectinomycin resistance and p15A origin of replication
Antibiotic Resistance(s)
- Spectinomycin
- Chloramphenicol
Fluorescent Marker
- GFP, constitutively expressed.
Can produce methionine?
- Yes, with my wild type genomic MetE gene.
Which experiments was I involved in?
- Can we reliably measure 2 populations in co-culture?