Achievements

Dry lab

- Developed an electrochemical model which simulates oxidised or reduced species being formed at and diffusing away from an electrode surface - Developed a biological model which simulates the genetic response to the concentration of oxidised and reduced species on a single cell level - Developed an integrated model and fitted it to experimental data to estimate absolute parameters of the electrogenetic system - Designed and constructed an affordable electrode array to facilitate programmable spatial using the electrogenetic device. - Developed a user-friendly phone application for remote control of patterning using the electrode array.

Wet lab

- Generated the first aerobic electrogenetic device in E. coli - Optimised chemical conditions of the system to maximise the biological response of the electrogenetic device without significantly impacting cell viability. - Proved this electrogenetic can provide fine spatial control of gene expression using a cheap electrode set-up. - Created a library of electrogenetic parts for use in future electrogenetic project. - Assembled the electrogenetic library into X different constructs using the next-generation BASIC assembly method, which were then characterised. - Proved applications of electrogenetic devices in biocontainment and biomanifacturing. - Found evidence of a new phenazine molecule as an inducer, which was suggested to exist in prior literature.

Human practices and outreach

- Proved molecules in the system could be replaced with safer alternatives following experts’ concerns of the toxicity of the system. - Designed a Communinication Strategy Guide (CSG) to direct communication of our project with stakeholders - Desined an interactive app (LTAT) to foster communication of problems within a team

Collaborations

- Collaborated with X different teams from X different countries. - Proved the part library can be used for alternative devices to electrogenetic systems with Oxford University. - Ensured comparability of the part library with multiple assembly methods with Oxford University