Team:Imperial College/Hardware


Hardware

Why do we need Hardware?

Our genetic construct is sensitive to redox stress, which can be induced by redox metabolic by-products or by environmental factors. But perhaps the most interesting feature of our genetic construct is that it can be activated using electric potentials. To do this, specific hardware is needed to build an interface between electricity and cells.

Hardware Requirments

The electronic interface of the cell needs to provide enough current for the redox reactions to happen and this current needs to be tightly controlled. A potentiostat will be used for this and it will additionally allow us to monitor what is happening in the system as well. A 3 electrode setup will be required in order to have an performant current supply to the system at the redox potentials of the cells. Further on, if the system proves to be successful, an electrode array would be built in order to explore spatially controlled gene expression and patterning.

PCB

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Part number Part description Required units
 GBP/unit Total price (GBP) Used Supplier Manufacturer Manufacturer Part number
1 Printed Circuit Board (Gold-plated) 1 67.69 67.79 Beta Layout Inc preferred none
2 4-to-16 Demultiplexer 1 1.33 1.33 DigiKey Nexperia USA Inc. 74HCT4514D,653
3 12 Output
Serial-Input-Parallel-Output
Shift Register		 1 1.32 1.32 DigiKey Nexperia USA Inc. NPIC6C4894PW-Q100J
4 Single Output Data Flip Flop 100 0.103 10.3 Farnell Element 14 NEXPERIA 74AHC1G79GW,125
5 Dual N-P MOSFET 100 0.265 26.5 Farnell Element 14 NEXPERIA PMCPB5530X
6 Operational Amplifier 1 0.967 0.97 Farnell Element 14 Texas Instruments LMV651MG
7 2.5 Volts - Voltage Regulator 1 1.2 1.2 DigiKey Texas Instruments LP38690SD-2.5/NOPB
8 1 \μF Capacitor 2 0.058 0.12 Farnell Element 14 TDK C1608X5R1C105K080AA
9 100 nF Capacitor 100 0.103 10.3 Farnell Element 14 MURATA GCJ188R71H104KA12D
10 0 \Ω Resistor 1 0.0273 0.27 Farnell Element 14 Panasonic Electronic
Components		 ERJ3GEY0R00V
Total Programmable 10x10
Electrode Array		 1 / 120.1 PixCell Electronics
Team		 / /
Part number Part description Link
1 Printed Circuit Board (Gold-plated) Beta Layout
2 4-to-16 Demultiplexer DigiKey - Shift Register
3 12 Output
Serial-Input-Parallel-Output
Shift Register		 DigiKey - Demultiplexer
4 Single Output Data Flip Flop Farnell - FlipFlop
5 Dual N-P MOSFET Farnell - MOSFET
6 Operational Amplifier Farnell - Op-Amp
7 2.5 Volts - Voltage Regulator DigiKey - 2.5VR
8 1 \uF Capacitor Farnell - C_1\u
9 100 nF Capacitor Farnell - 100nF
10 0 \Ohm Resistor Farnell - R_0\Ohm
Total Programmable 10x10
Electrode Array
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