Team:Bielefeld-CeBiTec/Parts
Parts
The iGEM parts registry includes more than 20,000 BioBricks. The standardized form of BioBricks are the basis so all present and future iGEM teams can build on existing BioBricks to achieve their projects. This year we integrated more than 50 existing BioBricks into our project and we submitted more than 80 BioBricks. Further iGEM teams can realize their project by building on our BioBricks.
All of our submitted basic parts are listed in Table 1.
We are very proud of our favorite basic part BBa_K2638002, an impressive copper importer.
The best composite of our team BBa_K2638703 is a second generation targed vector for siRNA silencing. All other composite parts are listed in table 2.
Our part improvement is a modified human ferritin BBa_K2638999. The human ferritin was submitted by iGEM Calgary in 2013 and is an iron storage protein. We changed certain amino acid residues to add the ability to form silver and gold nanoparticles.
During our project we had to deal with gene expression, which requires a certain promoter strength. To enable a comparison of different promoter strengths, we developed a measurement vector. Furthermore, we characterized the system with our promoter-RBS library.
The vector and the library are submitted as best part collection. The BioBricks are: BBa_K2638560 and BBa_K2638400 - BBa_K2638426.
| Identifier | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K2638001 | Coding | CopC coding sequence | Erika Schneider | 387 |
| BBa_K2638002 | Coding | CopD CDS | Erika Schneider | 912 |
| BBa_K2638000 | Coding | Heavy Metal Transporter (hmtA) | Erika Schneider | 1920 |
| BBa_K2638100 | Coding | alkyl hydroperoxide reductase, C22 subunit (ahpC) | Johannes Ruhnau | 564 |
| BBa_K2638101 | Coding | alkyl hydroperoxide reductase, F52a subunit (ahpF) | Johannes Ruhnau | 1566 |
| BBa_K2638103 | Coding | glutathione synthetase (gshB) | Johannes Ruhnau | 951 |
| BBa_K2638105 | Coding | catalase HPI (katG) | Johannes Ruhnau | 2181 |
| BBa_K2638106 | Coding | superoxide dismutase (sodA) | Johannes Ruhnau | 621 |
| BBa_K2638120 | Coding | glutathione peroxidase (btuE) | Johannes Ruhnau | 552 |
| BBa_K2638121 | Coding | glutathione reductase (gor) | Johannes Ruhnau | 1353 |
| BBa_K2638150 | Coding | Glutathione gamma-glutamylcysteinyltransferase 1 | Matthias Otto | 1458 |
| BBa_K2638151 | Coding | Glutathione gamma-glutamylcysteinyltransferase 1 with RBS and pTet promoter | Matthias Otto | 1535 |
| BBa_K2638152 | Coding | Glutathione gamma-glutamylcysteinyltransferase 1 with RBS and Anderson promoter | Matthias Otto | 1519 |
| BBa_K2638153 | Coding | Glutathione gamma-glutamylcysteinyltransferase 1 with AraBAD promoter and RBS | Matthias Otto | 2694 |
| BBa_K2638200 | Coding | OprC (TonB dependent copper transport porin) | Jakob Zubek | 2127 |
| BBa_K2638426 | Reporter | mRFP+ | Levin Joe Klages | 827 |
| BBa_K2638601 | Coding | Human Ferritin Heavy Chain Copper Enhanced Binding | Christoph Geske | 552 |
| BBa_K2638602 | Coding | Human Ferritin Heavy Chain E-Helix Removed Weaker Binding | Christoph Geske | 480 |
| BBa_K2638603 | Coding | Human Ferritin Heavy Chain Copper Enhanced Binding and E-Helix removed | Christoph Geske | 474 |
| BBa_K2638701 | Generator | Seamless siRNA Expression Vektor pGuide | Antonin Lenzen | 1780 |
| BBa_K2638702 | Generator | Seamless siRNA Expression Vektor for Hfq binding siRNAs | Antonin Lenzen | 1865 |
| BBa_K2638707 | Reporter | siRNA Target Vector with BFP and a GGGGS Linker | Antonin Lenzen | 2779 |
| BBa_K2638708 | Reporter | siRNA Target Vector with BFP and an EAAAK Linker | Antonin Lenzen | 2779 |
| BBa_K2638709 | Reporter | siRNA Target Vector with BFP and a cMyc Linker | Antonin Lenzen | 2779 |
| BBa_K2638710 | Reporter | siRNA Target Vector with BFP and XP Linker | Antonin Lenzen | 2779 |
| BBa_K2638711 | Reporter | siRNA Target Vector with AmilCP and a GGGGS Linker | Antonin Lenzen | 1226 |
| BBa_K2638712 | Reporter | siRNA Target Vector with AmilCP and EAAAK Linker | Antonin Lenzen | 1226 |
| BBa_K2638713 | Reporter | siRNA Target Vector with AmilCP and a cMyc Linker | Antonin Lenzen | 1226 |
| BBa_K2638714 | Reporter | siRNA Target Vector with AmilCP and XP Linker | Antonin Lenzen | 1226 |
| BBa_2638750 | Regulatory | Synthetic Origin of Replication A | Antonin Lenzen | 1 |
| BBa_K2638751 | Regulatory | Synthetic Origin of Replication B | Antonin Lenzen | 1 |
| BBa_K2638752 | Regulatory | pLac including a TetO sequence in place of the CAP binding sequence | Antonin Lenzen | 206 |
| BBa_K2638753 | Regulatory | pLac including a Tet operator sequence behind the Lac operator | Antonin Lenzen | 213 |
| BBa_K2638754 | Coding | E. coli Bacterioferritin Bfr | Antonin Lenzen | 477 |
| BBa_K2638755 | Coding | Ferredoxin reductase | Antonin Lenzen | 747 |
| BBa_K2638756 | Coding | Bacterial non-heme ferritin FtnA from Escherichia coli | Antonin Lenzen | 498 |
| BBa_K2638757 | Coding | Ferric Uptake Regulator (Fur) from Escherichia coli K12 | Antonin Lenzen | 447 |
| BBa_K2638758 | Generator | Seamless siRNA Expression Vektor for Omp-Utr protected siRNAs | Antonin Lenzen | 1897 |
| BBa_K2638759 | Generator | Seamless siRNA Expression Vektor with an Hfq binding tag and OmpA 5'-UTR | Antonin Lenzen | 1982 |
| BBa_K2638990 | Coding | Mutated Human Ferritin Heavy Chain (Backup) | Vanessa Krämer | 549 |
| BBa_K2638999 | Coding | Mutated Human Ferritin Heavy Chain | Vanessa Krämer | 552 |
