Team:William and Mary/Parts
Parts
Besides our 3G parts, library we are submitting a series of heat inducible reporters(mScarlet) and protease(mf-Lon) which we are using to construct IFFLs with different parameters.
Heat inducible IFFLs
| K2680064 | J23107 TPL + ptlpA B0034m mScarlet-I pdt-3d thrL terminator + ptlpAr BCD8 mf-Lon ssrA |
| K2680069 | J23107 TPL + ptlpA B0034m mScarlet-I thrL terminator + ptlpAr BCD8 mf-Lon ssrA |
| K2680063 | J23107 TPL + ptlpA B0034m mScarlet-I pdt-3b thrL terminator + ptlpAr BCD8 mf-Lon ssrA |
Heat inducible protease
| K2680050 | J23107 ts-CI + R0051 mf-Lon |
| K2680057 | J23100 ts-CI + R0051 mf-Lon ssrA |
| K2680058 | J23107 TPL + ptlpA BCD8 mf-lon thrL |
| K2680059 | J23107 TPL + ptlpA BCD8 mf-lon ssrA thrL |
| K2680060 | J23107 TPL + ptlpAr BCD8 mf-lon thrL |
| K2680061 | J23107 TPL + ptlpAr BCD8 mf-lon ssrA thrL |
Heat inducible reporters
| K2680051 | J23107 ts-CI + R0051 mScarlet-I pdt#3 |
| K2680052 | J23107 ts-CI + R0051 mScarlet-I pdt#3b |
| K2680053 | J23100 ts-CI + R0051 mScarlet-I pdt#3 |
| K2680054 | J23100 ts-CI + R0051 mScarlet-I pdt#3b |
| K2680062 | J23107 TPL + ptlpA b0034m mScarlet-I pdt#3 |
| K2680065 | J23107 TPL + ptplAr B0034 mScarlet-I pdt#3 |
| K2680066 | J23107 TPL + ptplA B0034 mScarlet-I pdt#3b |
| K2680067 | J23107 TPL + ptplAr B0034 mScarlet-I pdt#3b |
| K2680068 | J23107 ts-CI + R0051 mScarlet-I |
| K2680070 | J23107 ts-CI + R0051 mScarlet-I pdt#3e |
| K2680071 | J23107 TPL + ptlpA b0034m mScarlet-I pdt#3e |