Team:NUDT CHINA/InterLab
Introduction
The InterLab this year has been updated to a more detailed protocol. During the InterLab study, we used the LUDOX, fluorescence and the plasmids that were shipped along with the distribution kit, and closely followed the InterLab study protocol.
Through the InterLab 2018 study, we experienced a lot in the measuring work, and surprisingly got to know more about the instruments that usually were ignored by us. Actually, it is the measuring instruments that help us complete the project every year!!
Methods and Design
1. InterLab Parts
Positive Control (BBa_I20270)
NegativeControl (BBa_R0040)
Test Device1 (BBa_J364000)
Test Device2 (BBa_J364001)
Test Device3 (BBa_J364002)
Test Device4 (BBa_J364007)
Test Device5 (BBa_J364008)
Test Device6 (BBa_J364009)
2. Preparation
To start with, our team transformed E.coli strain DH5α with the provided plasmids, namely Test Device 1,2,3,4,5,6, Positive Control, and Negative Control. As long as colonies had emerged on Cm+ Resistance Media, we picked up 2 colonies in each petrie dish and cultured them at 37℃ with 220 rpm frequency for 14 hours. When the bacteria solutions were turbid enough, we began the following process.
3. OD600 Reference Point
With plate reader, we measured Abs600 of the LUDOX and H2O. The H2O measurement served as the background. Both included 4 technical replicates to enhance the reliability of the results. Comparing to the standard OD600 reference given, which was 0.0425, we were able to achieve a ratio between OD600 and Abs600. The ratio was essential in converting Abs600 raw measurements into standard OD600 records.