Team:NUDT CHINA/InterLab
Development of A Novel
Blood-MicroRNA Handy Detection System with CRISPR
InterLab
Introduction
The InterLab this year has been updated to a more detailed protocol. During the InterLab study, we used the LUDOX, fluorescence and the plasmids that were shipped along with the distribution kit, and closely followed the InterLab study protocol.
Through the InterLab 2018 study, we experienced a lot in the measuring work, and surprisingly got to know more about the instruments that usually were ignored by us. Actually, it is the measuring instruments that help us complete the project every year!!
Methods and Design
1. InterLab Parts
Positive Control (BBa_I20270)
NegativeControl (BBa_R0040)
Test Device1 (BBa_J364000)
Test Device2 (BBa_J364001)
Test Device3 (BBa_J364002)
Test Device4 (BBa_J364007)
Test Device5 (BBa_J364008)
Test Device6 (BBa_J364009)
2. Preparation
To start with, our team transformed E.coli strain DH5α with the provided plasmids, namely Test Device 1,2,3,4,5,6, Positive Control, and Negative Control. As long as colonies had emerged on Cm+ Resistance Media, we picked up 2 colonies in each petrie dish and cultured them at 37℃ with 220 rpm frequency for 14 hours. When the bacteria solutions were turbid enough, we began the following process.
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This is the sign of the famous game!
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This is the sign of the famous game!
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| name | city | |
|---|---|---|
| Tanmay | Bangalore | 560001 |
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