Difference between revisions of "Team:IIT Delhi/Basic Part"

 
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<table>
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<div class="divTable">
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  <tr>
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<div class="divTableBody">
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    <th>S. No</th>
−
<div class="divTableRow">
+
    <th>Part Name</th>
−
<div class="divTableCell">&nbsp;S. No</div>
+
    <th>BioBrick</th>
−
<div class="divTableCell">Part Name&nbsp;</div>
+
    <th>Description</th>
−
<div class="divTableCell">BioBrick&nbsp;</div>
+
    <th>Part Type</th>
−
<div class="divTableCell">Description&nbsp;</div>
+
  </tr>
−
<div class="divTableCell">Part Type&nbsp;</div>
+
  <tr>
 +
    <td>1</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2814001"><span style="font-weight: 400;">BBa_K2814001</span></a></td>
 +
    <td>pLTL</td>
 +
    <td>Lac-Tet-Lac Hybrid Promoter</td>
 +
    <td>Promoter</td>
 +
  </tr>
 +
  <tr>
 +
    <td>2</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2814007">BBa_K2814007</a></td>
 +
    <td>sfGFP_ssrA</td>
 +
    <td>Reporter gene (codon-optimised for E. coli)</td>
 +
    <td>Coding Sequence</td>
 +
  </tr>
 +
  <tr>
 +
    <td>3</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2814008">BBa_K2814008</a></td>
 +
    <td>rrnB T1 Terminator + T7Te Terminator</td>
 +
    <td>Double Transcriptional Terminator</td>
 +
    <td>Terminator</td>
 +
  </tr>
 +
  <tr>
 +
    <td>4</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2814009">BBa_K2814009</a></td>
 +
    <td>mKate2</td>
 +
    <td>Reporter gene (codon-optimised for E. coli)</td>
 +
    <td>Coding Sequence</td>
 +
  </tr>
 +
  <tr>
 +
    <td>5</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2814010">BBa_K2814010</a></td>
 +
    <td>rrnB T1 Terminator</td>
 +
    <td>Transcriptional Terminator</td>
 +
    <td>Terminator</td>
 +
  </tr>
 +
  <tr>
 +
    <td>6</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2814011">BBa_K2814011</a></td>
 +
    <td>attP TP901-1</td>
 +
    <td>Attachment site for TP901-1 Integrase</td>
 +
    <td>DNA</td>
 +
  </tr>
 +
  <tr>
 +
    <td>7</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2814012">BBa_K2814012</a></td>
 +
    <td>BxbI Integrase + ssrA(LVA) deg tag</td>
 +
    <td>CDS for Integrase Bxb1</td>
 +
    <td>Coding Sequence</td>
 +
  </tr>
 +
  <tr>
 +
    <td>8</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2814013">BBa_K2814013</a></td>
 +
    <td>attB TP901-1</td>
 +
    <td>Attachment site for TP901-1 Integrase</td>
 +
    <td>DNA</td>
 +
  </tr>
 +
  <tr>
 +
    <td>9</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2814014">BBa_K2814014</a></td>
 +
    <td>complement of B0034, RBS on the antisense strand</td>
 +
    <td>Ribosome Binding Site</td>
 +
    <td>RBS</td>
 +
  </tr>
 +
  <tr>
 +
    <td>10</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2814015">BBa_K2814015</a></td>
 +
    <td>pLac(lambda) hybrid</td>
 +
    <td>Lac-Lambda Hybrid Promoter</td>
 +
    <td>Promoter</td>
 +
  </tr>
 +
  <tr>
 +
    <td>11</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2814017">BBa_K2814017</a></td>
 +
    <td>attP Bxb1</td>
 +
    <td>Attachment site for Bxb1 Integrase</td>
 +
    <td>DNA</td>
 +
  </tr>
 +
  <tr>
 +
    <td>12</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2814018">BBa_K2814018</a></td>
 +
    <td>attB Bxb1</td>
 +
    <td>Attachment site for Bxb1 Integrase</td>
 +
    <td>DNA</td>
 +
  </tr>
 +
  <tr>
 +
    <td>13</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2814019">BBa_K2814019</a></td>
 +
    <td>P7 Promoter</td>
 +
    <td>Constitutive P7 promoter</td>
 +
    <td>Promoter</td>
 +
  </tr>
 +
  <tr>
 +
    <td>14</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2814021">BBa_K2814021</a></td>
 +
    <td>BxbI-Xis + ssrA(LVA) deg tag</td>
 +
    <td>CDS for Recombinase Directionality Factor for Integrase Bxb1</td>
 +
    <td>Coding Sequence</td>
 +
  </tr>
 +
  <tr>
 +
    <td>15</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2814022">BBa_K2814022</a></td>
 +
    <td>attB BxbI (reverse orientation)</td>
 +
    <td>Attachment site for Bxb1 Integrase (Reverse)</td>
 +
    <td>DNA</td>
 +
  </tr>
 +
  <tr>
 +
    <td>16</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2814025">BBa_K2814025</a></td>
 +
    <td>TP901-1 Integrase</td>
 +
    <td>CDS for Integrase TP901-1</td>
 +
    <td>Coding Sequence</td>
 +
  </tr>
 +
</table>  
 
</div>
 
</div>
−
<div class="divTableRow">
 
−
<div class="divTableCell">1</div>
 
−
<div class="divTableCell">
 
−
<p><a href="http://parts.igem.org/Part:BBa_K2814001"><span style="font-weight: 400;">BBa_K2814001</span></a></p>
 
−
</div>
 
−
<div class="divTableCell">pLTL&nbsp;</div>
 
−
<div class="divTableCell">Lac-Tet-Lac Hybrid Promoter</div>
 
−
<div class="divTableCell">Promoter&nbsp;</div>
 
−
</div>
 
−
<div class="divTableRow">
 
−
<div class="divTableCell">2&nbsp;</div>
 
−
<div class="divTableCell">
 
−
<p><a href="http://parts.igem.org/Part:BBa_K2814007">BBa_K2814007</a></p>
 
−
</div>
 
−
<div class="divTableCell">sfGFP_ssrA&nbsp;</div>
 
−
<div class="divTableCell">Reporter gene (codon-optimised for E. coli)&nbsp;</div>
 
−
<div class="divTableCell">Coding Sequence&nbsp;</div>
 
−
</div>
 
−
<div class="divTableRow">
 
−
<div class="divTableCell">3&nbsp;</div>
 
−
<div class="divTableCell">
 
−
<p><a href="http://parts.igem.org/Part:BBa_K2814008">BBa_K2814008</a></p>
 
−
</div>
 
−
<div class="divTableCell">rrnB T1 Terminator + T7Te Terminator&nbsp;</div>
 
−
<div class="divTableCell">Double Transcriptional Terminator&nbsp;</div>
 
−
<div class="divTableCell">Terminator&nbsp;</div>
 
−
</div>
 
−
<div class="divTableRow">
 
−
<div class="divTableCell">4&nbsp;</div>
 
−
<div class="divTableCell">
 
−
<p><a href="http://parts.igem.org/Part:BBa_K2814009">BBa_K2814009</a></p>
 
−
</div>
 
−
<div class="divTableCell">mKate2&nbsp;</div>
 
−
<div class="divTableCell">Reporter gene (codon-optimised for E. coli)&nbsp;&nbsp;</div>
 
−
<div class="divTableCell">Coding Sequence&nbsp;</div>
 
−
</div>
 
−
<div class="divTableRow">
 
−
<div class="divTableCell">5&nbsp;</div>
 
−
<div class="divTableCell">
 
−
<p><a href="http://parts.igem.org/Part:BBa_K2814010">BBa_K2814010</a></p>
 
−
</div>
 
−
<div class="divTableCell">rrnB T1 Terminator&nbsp;</div>
 
−
<div class="divTableCell">Transcriptional Terminator</div>
 
−
<div class="divTableCell">Terminator&nbsp;</div>
 
−
</div>
 
−
<div class="divTableRow">
 
−
<div class="divTableCell">6&nbsp;</div>
 
−
<div class="divTableCell">
 
−
<p><a href="http://parts.igem.org/Part:BBa_K2814011">BBa_K2814011</a></p>
 
−
</div>
 
−
<div class="divTableCell">attP TP901-1&nbsp;</div>
 
−
<div class="divTableCell">Attachment site for TP901-1 Integrase&nbsp;</div>
 
−
<div class="divTableCell">DNA&nbsp;</div>
 
−
</div>
 
−
<div class="divTableRow">
 
−
<div class="divTableCell">7&nbsp;</div>
 
−
<div class="divTableCell">
 
−
<p><a href="http://parts.igem.org/Part:BBa_K2814012">BBa_K2814012</a></p>
 
−
</div>
 
−
<div class="divTableCell">BxbI Integrase + ssrA(LVA) deg tag&nbsp;</div>
 
−
<div class="divTableCell">CDS&nbsp;for Integrase Bxb1&nbsp;&nbsp;</div>
 
−
<div class="divTableCell">Coding Sequence&nbsp;</div>
 
−
</div>
 
−
<div class="divTableRow">
 
−
<div class="divTableCell">8&nbsp;</div>
 
−
<div class="divTableCell">
 
−
<p><a href="http://parts.igem.org/Part:BBa_K2814013">BBa_K2814013</a></p>
 
−
</div>
 
−
<div class="divTableCell">attB TP901-1&nbsp;</div>
 
−
<div class="divTableCell">Attachment site for&nbsp;TP901-1 Integrase&nbsp;</div>
 
−
<div class="divTableCell">DNA&nbsp;</div>
 
−
</div>
 
−
<div class="divTableRow">
 
−
<div class="divTableCell">9&nbsp;</div>
 
−
<div class="divTableCell">
 
−
<p><a href="http://parts.igem.org/Part:BBa_K2814014">BBa_K2814014</a>&nbsp;</p>
 
−
</div>
 
−
<div class="divTableCell">complement of B0034, RBS on the antisense strand&nbsp;</div>
 
−
<div class="divTableCell">Ribosome Binding Site&nbsp;</div>
 
−
<div class="divTableCell">RBS&nbsp;</div>
 
−
</div>
 
−
<div class="divTableRow">
 
−
<div class="divTableCell">10&nbsp;</div>
 
−
<div class="divTableCell">
 
−
<p><a href="http://parts.igem.org/Part:BBa_K2814015">BBa_K2814015</a></p>
 
−
</div>
 
−
<div class="divTableCell">pLac(lambda) hybrid&nbsp;</div>
 
−
<div class="divTableCell">Lac-Lambda Hybrid Promoter&nbsp;</div>
 
−
<div class="divTableCell">Promoter</div>
 
−
</div>
 
−
<div class="divTableRow">
 
−
<div class="divTableCell">11&nbsp;</div>
 
−
<div class="divTableCell">
 
−
<p><a href="http://parts.igem.org/Part:BBa_K2814017">BBa_K2814017</a></p>
 
−
</div>
 
−
<div class="divTableCell">attP Bxb1&nbsp;</div>
 
−
<div class="divTableCell">Attachment site for Bxb1 Integrase&nbsp;</div>
 
−
<div class="divTableCell">DNA&nbsp;</div>
 
−
</div>
 
−
<div class="divTableRow">
 
−
<div class="divTableCell">12&nbsp;</div>
 
−
<div class="divTableCell">
 
−
<p><a href="http://parts.igem.org/Part:BBa_K2814018">BBa_K2814018</a></p>
 
−
</div>
 
−
<div class="divTableCell">attB Bxb1&nbsp;</div>
 
−
<div class="divTableCell">Attachment site for Bxb1 Integrase&nbsp;</div>
 
−
<div class="divTableCell">DNA&nbsp;</div>
 
−
</div>
 
−
<div class="divTableRow">
 
−
<div class="divTableCell">13&nbsp;</div>
 
−
<div class="divTableCell">
 
−
<p><a href="http://parts.igem.org/Part:BBa_K2814019">BBa_K2814019</a></p>
 
−
</div>
 
−
<div class="divTableCell">P7 Promoter&nbsp;</div>
 
−
<div class="divTableCell"><span style="font-weight: 400;">Constitutive P7 promoter</span>&nbsp;</div>
 
−
<div class="divTableCell">Promoter</div>
 
−
</div>
 
−
<div class="divTableRow">
 
−
<div class="divTableCell">14</div>
 
−
<div class="divTableCell">
 
−
<p><a href="http://parts.igem.org/Part:BBa_K2814021">BBa_K2814021</a>&nbsp;</p>
 
−
</div>
 
−
<div class="divTableCell">BxbI-Xis &nbsp;+ ssrA(LVA) deg tag&nbsp;</div>
 
−
<div class="divTableCell">CDS for Recombinase Directionality Factor for Integrase Bxb1&nbsp;</div>
 
−
<div class="divTableCell">Coding Sequence&nbsp;</div>
 
−
</div>
 
−
<div class="divTableRow">
 
−
<div class="divTableCell">15</div>
 
−
<div class="divTableCell">
 
−
<p><a href="http://parts.igem.org/Part:BBa_K2814022">BBa_K2814022</a>&nbsp;</p>
 
−
</div>
 
−
<div class="divTableCell">attB BxbI (reverse orientation)&nbsp;</div>
 
−
<div class="divTableCell">Attachment site for Bxb1 Integrase (Reverse)&nbsp;</div>
 
−
<div class="divTableCell">DNA&nbsp;</div>
 
−
</div>
 
−
<div class="divTableRow">
 
−
<div class="divTableCell">16</div>
 
−
<div class="divTableCell"><a href="http://parts.igem.org/Part:BBa_K2814025">BBa_K2814025</a>
 
−
<p>&nbsp;</p>
 
−
</div>
 
−
<div class="divTableCell">
 
−
<p><span style="font-weight: 400;">TP901-1 Integrase</span>&nbsp;</p>
 
−
</div>
 
−
<div class="divTableCell">CDS for Integrase TP901-1</div>
 
−
<div class="divTableCell">Coding Sequence</div>
 
−
</div>
 
−
</div>
 
−
</div>
 
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      <p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;"> We faced some problems during our InterLab characterization, our absorbance at 600nm for silica beads at high dilutions didn't follow the trend. Upon which, we discussed the problem on All India iGEM group, where the teams gave us multiple suggestions and shared their protocols by which we were able to obtain our results as deisred by iGEM. Hence, we would like to express our gratitude towards the Indian iGEM teams.
 
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Latest revision as of 23:40, 17 October 2018

iGEM IIT Delhi

Notebook
Lab Records Medal Criteria
Team
Members Attributions
Collaboration
Human Practices
Human Practices Safety Public Engagement
Modelling
Parts
Basic Parts Composite Parts
Results
Demonstrate Interlab
Project
Overview Design
S. No Part Name BioBrick Description Part Type
1 BBa_K2814001 pLTL Lac-Tet-Lac Hybrid Promoter Promoter
2 BBa_K2814007 sfGFP_ssrA Reporter gene (codon-optimised for E. coli) Coding Sequence
3 BBa_K2814008 rrnB T1 Terminator + T7Te Terminator Double Transcriptional Terminator Terminator
4 BBa_K2814009 mKate2 Reporter gene (codon-optimised for E. coli) Coding Sequence
5 BBa_K2814010 rrnB T1 Terminator Transcriptional Terminator Terminator
6 BBa_K2814011 attP TP901-1 Attachment site for TP901-1 Integrase DNA
7 BBa_K2814012 BxbI Integrase + ssrA(LVA) deg tag CDS for Integrase Bxb1 Coding Sequence
8 BBa_K2814013 attB TP901-1 Attachment site for TP901-1 Integrase DNA
9 BBa_K2814014 complement of B0034, RBS on the antisense strand Ribosome Binding Site RBS
10 BBa_K2814015 pLac(lambda) hybrid Lac-Lambda Hybrid Promoter Promoter
11 BBa_K2814017 attP Bxb1 Attachment site for Bxb1 Integrase DNA
12 BBa_K2814018 attB Bxb1 Attachment site for Bxb1 Integrase DNA
13 BBa_K2814019 P7 Promoter Constitutive P7 promoter Promoter
14 BBa_K2814021 BxbI-Xis + ssrA(LVA) deg tag CDS for Recombinase Directionality Factor for Integrase Bxb1 Coding Sequence
15 BBa_K2814022 attB BxbI (reverse orientation) Attachment site for Bxb1 Integrase (Reverse) DNA
16 BBa_K2814025 TP901-1 Integrase CDS for Integrase TP901-1 Coding Sequence

Contact us

Address

Undergraduate Laboratory
Department of Biotechnology and Biochemical Engineering, IIT Delhi