Team:BIT-China/JudgingForm
1. Team registration.
2. Team wiki.
3. Complete judging form.
4. Poster & presentation at the giant jamboree.
5. Safety form.
1. Parts: Convince the judges that at least one new BioBrick Part of your own design that is related to your project works as expected.
We have verified 16 new parts in total. Our favorite part BBa_K2765021can overexpress gene yno1 to accumulate the intracellular ROS, which is crucial for our project.
2. Collaborations Convince the judges you have significantly worked with one (or more) currently registered 2018 iGEM team(s) in a meaningful way.
We have established cooperation with Tsinghua-A, FJNU-China and Peking. See our collaboration.
1. Integrated Human Practices.
We have completed communication with the World's Top Five Hundred food company COFCO and pharmaceutical enterprise Wehand-bio, whose researchers have given professional advice on our project and improved it.
2. Improve a previous part.
We optimized Connell team’s previous part BBa_K2296006and obtained our core part BBa_K2296006 whichcan produce redox-sensitive Green fluorescent protein roGFP2-Orp1 and display ROS content in cells by its fluorescence ratio.
We established three models, H2O2 Decomposition Model, Fluorescent Probe Modeland roGFP2-Orp1 Michaelis equation Model, which can the convert the extracellular H2O2 concentration, fluorescence intensity of DFCH-DA probe and fluorescence intensity of roGFP2-orp1 protein into the intracellular H2O2 concentration.
This year, we created a detecting system for antioxidants. It is unprecedented to solve this difficult problem by synthetic biological strategy.
See our results page. Our system gave a similar result to CAA assay in three selected antioxidant tests，which proves our system can detect the antioxidant in living cell successfully!
This year, we want to fight for the Best Applied Design Special Prize.
Oxidation damage, aging and relative diseases are global concerns which highly relate to human health and the sole treatment is antioxidant. However, detection approaches to antioxidant are quite limited, especially those for the living-cells. Traditional methods focused more on direct redox reaction which may have non-proper navigation to living systems. Living cells are integrated with multiple natural anti-oxidant systems, including anti-oxidant enzyme system, reductive system, post-damage repair system and etc, which makes it sophisticated to evaluate real effects from exogenous antioxidant. The distinctions in living cells make some huge cost on dining antioxidant senseless. To solve this real-world problem, a corresponding “living antioxidant detection device” was built via synthetic biology manners this year. Yeast was set as our host cell as it can present a simple but accurate measuring platform for us. Then, we constructed multiple functional gene circuits to implement ROS regulating, endogenous redox reaction testing, feedback regulation and so on. Our goal is to make it easily, accurately, fast and economically to detect antioxidant, and contribute to world real feasible antioxidants.
Introduce our favorite parts for you.
BBa_K2765021, this guy can increase the intracellular ROS content through overexpressing yno1 gene. He has replaced the position of artificial synthetic oxyradical in the previous antioxidant detection methods. We can’t start detecting without he.
BBa_K2765052, this girl can express redox-sensitive green fluorescent protein roGFP2-Orp1 which can display ROS content in cells by its fluorescence ratio. She plays the role of signal output in our system and has replaced the position of chemical probe. She has helped us save a lot of money for fluorescent probes. Thank her.
BBa_K2765050,BBa_K2765051,BBa_K2765052,BBa_K2765053. This family is our brilliant part collection.
In our system, we used roGFP2-Orp1, which can reflect the ROS level in cell immediately through the rate of the concentration of two kinds of protein states, as our signal output. But according to this measurement principle, the concentration of whole roGFP2-Orp1 can effect the final signal output strength, even detect the same sample. So, we built a series of gene circuits with different transcription level promoters to express roGFP2-Orp1 and hoped to solve this interfere. Four common promoters were chosen, FBA1, TEF2, TEF1, ENO2. We tested four gene circuits and found different promoters could work as expected. Considering the application of our system in the future, we made this collection of these four parts and wished these parts can be used in different antioxidant detection.
