Team:CPU CHINA/Notebook/Log

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Early Stages

As is shown in Human Practice, we China Pharmaceutical University has a club for synthetic biology and iGEM. This year’s team started from Kexin Cao, Yunge Lou, Junqing Zhang, Shuyao Zhou and Huandi Xu. These 5 have long been club members of CPU Bio-X Club.They witnessed the success of their senior schoolmates and was determined and ready for this year’s competition. After intense group study and brainstorming for 4 months, Shuyao Zhou’s scheme of condition RNA interference (though very rough compared with today’s whole project) was the most feasible one, and was approved by our PI Prof.Tian.

12/2-12/9/2017

Yunge Lou and Yali Wu decide on the site-directed mutagenesis of hTERT and HULC promoter sequences. (Design) Yunge Lou and Yali Wu think about possible numerical models. (Model) Huandi Xu and Junqing Zhang research on the function of MAP4K4 gene, how the tumor reacts after the gene is silenced and the transcription of inhibitory strand. (Design) Xingnuo Cheng thinks about the experiment protocol for subcutaneous tumorigenesis in nude mice. (Experiment) Shuyao Zhou and Kexin Cao research on a proper RNA-dependent RNA polymerase (RdRP). (Design)

12/10-12/12/2017

Yunge Lou researches onligand-inducible promoter/RdRP inhibitors. (Design) Yali wu determines the exact sequence of hTERT and HULC promoter. (Design) Huandi Xu researches on the mechanism of MAP4K4 in hepatocellular carcinoma. (Design) Kexin Cao researches on the analytical method for hepatitis C virus RdRP. (Experiment) Junqing Zhang chooses a vector to express double ncRNA. (Design) Shuyao Zhou determines RdRP and ncRNA DNA sequences. (Design)

1/21-1/26/2018

Kexin Cao designs the miRNA sponge which should absorbthe microRNA targeting MAP4K4. (Design) Junqing Zhang chooses a vector to express double ncRNA. (Design) Yunge Lou researches on theCMV-tet-tTA system. (Design) Huandi Xu and Shuyao Zhou design the pSil-MAP4K4 and inhibitory strand. (Design) Xingnuo Cheng and Yali Wu write a summary about the project, including the principle, technical route and research main contents. (Design)

1/27-1/31/2018

Kexin Cao, Junqing Zhang, Yali Wu, Yunge Lou, Huandi Xu, Shuyao Zhou and Xingnuo Cheng make a budget for all the proposed experimental materials and reagents. (Experiment) Yunge Lou schedules the experiments. (Experiment) Xingnuo Cheng and Junqing Zhang prepare a schematic diagram. (Design)

Winter vacation

Sign up and pay the fees.

3/4-3/9/2018

Kexin Cao determines the exact sequence of the miRNA sponge. (Design) Shuyao Zhou combines the HULC promoter with the pSil-MAP4K4 sequence. (Design) Junqing Zhang calls for the synthesis of the parts of Plasmid 1. (Experiment)

3/10-3/20/2018

Junqing Zhang summarizes the exact sequences of parts proposed in Plasmid 2. (Design) Xingnuo Cheng, Yali Wu, Kexin Cao and Junqing Zhang try to give the project a name. (Design) Yunge Lou thinks about possible numerical models. (Model) We recruit new members. Nannan Liu and Wanli Zhang joined our team!

3/21-4/1/2018

Hongyu Wang, Yiyang Zhu, Peng Sun and Ying Miao joined us! Different responsibilities for each person can be seen in Team. Xingnuo Cheng summarizes the novelty of the proposed research project. (Design) Hongyu Wang thinks about possible projects for Human Practice (HP).We decide to give a speech through the university’s TED platform (TEDx CPU) and connect with the Cancer Rehabilitation Association in Nanjing. (HP) Wanli Zhang writes an experimental protocol for double digestion (digesting a DNA substrate with two restriction endonucleases simultaneously). (Experiment)

4/1-4/7/2018

Yali Wu and Shuyao Zhou propose the name “Rig” as our brand, short for Conditional RNA interference gene therapy. It is selected as the project’s official brand name upon a meeting.(Design) Huandi Xu and Hongyu Wang prepare for the cross-school talk held by Nanjing University. (HP) Xingnuo Cheng and Kexin Cao finish the opening presentation for “Training Program of Innovation and Entrepreneurship of College Students”. The project is now supported at provincial level (No. 201810316034X). (HP) Yali Wu and Yunge Lou research on general value assessment method for cancer treatment, as a direction for us to conduct human practice. (Model)

4/14-4/22/2018

Continuing from last week’s work, Yali Wu and Yunge Lou investigate the pharmacological property of two first-line macromolecular drugs, both delivered with liposome.(Model) Junqing Zhang and Ying Miao design the team uniform and team card. They try to integrate some Norse mythology and aesthetic into the design as our name Rig is quite Norse. Kexin Cao and Shuyao Zhou both design a team logo and we favor Cao’s. Xingnuo Cheng designs a scroll used for presentation. (Design) Huandi Xu starts to look for sponsorship. (Sponsor) Hongyu Wang helps to organize a synthetic biology themed orienteering with CPU Bio-X Club, as part of our human practice. (HP)

4/23-4/28/2018

Hongyu Wang draws up a plan of interviewing experts in different fields asin academia, industry and politics. (HP) Junqing Zhang and Ying Miao continue designing the team uniform. (Design) Yali Wu and Yunge Lou validate the model structure and design a questionnaire about treatment costs and side reaction. (Model) Huandi Xu meets and finally (on June 24th) agrees with Genscript (Nanjing, China) on a 2000 yuan sponsorship. (Sponsor)

Mid-term

Members including Shuyao Zhou, Huandi Xu, Kexin Cao, Xingnuo Cheng, Yunge Lou, Ying Miao, Hongyu Wang, Wanli Zhang and Yiyang Zhu decide to join the Giant Jamboree. At the same time some of them start applying for a US visa and an “Explore the World” scholarship from the univeristy’s office of international exchange and cooperation. Huandi Xu, Kexin Cao, Xingnuo Cheng, Yunge Lou, Ying Miao and Hongyu Wang each receives a scholarship of up to ￥20000 in July.

5/15-5/19/2018

Ying Miao and Yiyang Zhu start to think about the website’s frame. (Wiki) Peng Sun starts to learn video making and editing. (HP) Nannan Liu and Wanli Zhang extract hTERT promoter and tTA from the bacteria, order a pIRES vector. (Experiment) Yali Wu and Huandi Xu draft an essay about synthetic biology and prepare for the TEDx speech. (HP) Kexin Cao and Xingnuo Cheng translate this log until 4/28. (Wiki)

5/21-5/27/2018

Shuyao Zhou writes a proposal submission to Genscript. (Sponsor) Huandi Xu and Hongyu Wang help hold the orienteering event through CPU Bio-X Club. (HP) Yiyang Zhu puts the translated log on the website. (Wiki) Peng Sun makes a short beginning, which will be used in all our video materials. (HP) Nannan Liu and Wanli Zhang call for the synthesis of the PCR primer of NS5B. (Experiment)

5/28-6/3/2018

Yiyang Zhu and Ying Miao start to edit the website of our Human Practice, including Education & Public Engagement. (Wiki) Yunge Lou and Yali Wu test the Markov Model. (Model) Junqing Zhang and Wanli Zhang submit the sequence of Plasmid 2 by IDT FREE DNA synthesis. (Experiment) Nannan Liu connect shTERT to the pIRES vector. (Experiment) Xingnuo Cheng analyzes the statistic result of the survey conducted during the orienteering event.(HP) Kexin Cao and Shuyao Zhou summarize materials about CPU Bio-X Club and other activities. (HP)

6/4-6/17/2018

Peng Sun continue practicing video editing. (HP) Junqing Zhang draws a Science Sketch for iGEM NYU Abu Dhabi. (HP) Huandi Xu and Hongyu Wang collect materials from the TEDx speech and other activities. (HP) Yali Wu and Yunge Lou measure the general cost of treatments with FOLFOX4 and Sorafenib. (Model) Yiyang Zhu and Ying Miao start to edit the website of our Project and other available contents. (Wiki) Nannan Liu put shTERT promoter on pIRES, than transfect E. Coli with the plasmid. (Experiment)

6/18-6/23/2018

Yiyang Zhu and Ying Miao continue working on the website’s frame. (Wiki) Yunge Lou summarizes the principle of Markov Model. (Model) Group photo was taken on June 23th.

6/24-7/1/2018

Hongyu Wang, Shuyao Zhou and Kexin Cao interview the medicinal economist Yongjun Liu. (HP) Shuyao Zhou and Huandi Xu work onan initial version ofSafety form. Huandi Xu signs up for Interlab. (Design) Yiyang Zhu puts the Description on the home page of the team wiki. (Wiki) Wanli Zhang schedules the experiments after the final exams. (Experiment) Yunge Lou investigates the parts used by our 08 iGEM team. (Design)

Final exams

The DNA Distribution Kit arrives. Nannan Liu arranges the experiments during the summer vacation. (Experiment) Shuyao Zhou, Kexin Cao, Yunge Lou, Ying Miao, Hongyu Wang and Wanli Zhang go to Consulate General of the United States in Shanghai to continue applying for their visas.

7/17-7/27/2018

Xingnuo Cheng designs the infosheet “Liver Cancer Q&A”. (HP) Huandi Xu, on behalf of the team, signs up for the fifth Conference of China iGEMer Community (CCiC). Xingnuo Cheng, Shuyao Zhou and Yiyang Zhu translate this log and put it on wiki until 7/23. (Wiki) Huandi Xu finishes various questionnaires from the iGEM community (Survey To Fight Water Pollution, Invasive Species Survey, Burn General Knowledge Survey). (Collaboration) Kexin Cao and Junqing Zhang load U6-inhSi-pri-miRNA on pcDNA3.1. (Experiment) Kexin Cao and Hongyu Wang write the Business Plan. (HP) Wanli Zhang and Kexin Cao finish interlab study and upload the results. (InterLab)

7/28-8/7/2018

Ying Miao and Huandi Xu sign up as Rig for the Superhero League of iGEM Ruia-Mumbai. (Collaboration) Shuyao Zhou edits the team's InterLab wiki page and adds a summary of the work we conducted. (InterLab) Peng Sun works on an anime about the infosheet on the wiki. (wiki) Kexin Cao and Xingnuo Cheng link hulc with U6-inhSi-pri-miRNA on pcDNA3.1. (Experiment) Ying Miao and Yiyang Zhu finish the background of the Home page and other graphic design. (wiki) Wanli Zhang arranges his visa at Consulate General of the United States in Shanghai.

8/8-8/15/2018

Kexin Cao and Hongyu Wang continue writing the Business Plan. (HP) Shuyao Zhou and Peng Sun make subtitles for the TEDX video. (HP) Junqing Zhang design a scheme figure for description of construction of vectors. (wiki) Yunge Lou and Xingnuo Cheng link TRE-MAP4K4 miRNA sponge with hulc-pri-miRNA (MAP4K4)-U6-inhSi-pri-miRNA on pcDNA3.1. (Experiment) Peiyuan Gao starts making a virtual reality program for our project. Ying Miao and Yiyang Zhu design the interlab page and the Human Practice pages. (wiki)

8/16-8/23/2018

Kexin Cao and Hongyu Wang continue writing the Business Plan. (HP) Junqing Zhang makes our poster for CCiC. Huandi Xu, Xingnuo Cheng and Yali Wu prepare for the presentation at CCiC. Wanli Zhang and Kexin Cao link TRE-MAP4K4 miRNA sponge with hulc-pri-miRNA (MAP4K4)-U6-inhSi-pri-miRNA on pcDNA3.1. (Experiment) Ying Miao continues her graphic designs. (wiki) Hongyu Wang adds a summary of the work related to the Business Plan. (wiki) Peng Sun continues working on the anime about the infosheet. (wiki) Huandi Xu and Wanli Zhang arrange their visas at Consulate General of the United States in Shanghai.

8/24-8/31/2018

Huandi Xu obtains water samples for NWU-CHINA. (collaboration) Huandi Xu, Kexin Cao and Junqing Zhang work on the PowerPoint for CCiC presentation. Shuyao Zhou and Huandi Xu starts adding and documenting the major parts. (parts) Wanli Zhang and Yunge Lou link TRE-MAP4K4 miRNA sponge with hulc-pri-miRNA (MAP4K4)-U6-inhSi-pri-miRNA on pcDNA3.1. (Experiment) Wanli Zhang prepares the materials to be used in later verification of the parts. (Experiment) Shuyao Zhou and Yiyang Zhu translate this log and put it on wiki until 8/31. (Wiki) Huandi Xu, Yali Wu, Xingnuo Cheng and Peng Sun attend the CCiC, from the 27th to the 31st.

9/1/2018-9/16/2018

Junqing Zhang and Ying Miao design the team banner for Giant Jamboree. Junqing Zhang, Shuyao Zhou and Peng Sun finish the science sketch for NYU Abu Dhabi. (HP) Hongyu Wang, Yunge Lou and Huandi Xu interview the Rehabilitation Association of Nanjing CHINA. (HP) Wanli Zhang, Kexin Cao, Yunge Lou and Huandi Xu build the vectors: pIRES-tTA-(NLS)NS5B, plasmids for Parts submission and plasmids for promoter identification. (Experiment) Yiyang Zhu sets up the web page for human practice. (Wiki) Shuyao Zhou and Huandi Xu fill out and submit the safety form. Xingnuo Cheng translates the article of interviewing Pharmaco-Economic experts. (HP)

9/17/2018-9/30/2018

Ying Miao makes the team flags. Huandi Xu submits the Team Roster. Kexin Cao, Junqing Zhang and Yali Wu finish building pIRES-tTA-(NLS)NS5B vector. (Experiment) Kexin Cao, Yunge Lou and Wanli Zhang build the vectors: PCDNA-hulc-psil-U6-inhsi, plasmids for parts submission and plasmids for promoter identification. (Experiment) Hongyu Wang and Huandi Xu write a summary for HP as well as a summary for interviewing the Rehabilitation Association of Nanjing CHINA. Peng Sun cuts the video of interviewing the Rehabilitation Association of Nanjing CHINA. (HP) Peng Sun, Yali Wu and Yiyang Zhu subtitle the video of interviewing Pharmaco-Economic experts. (HP) Huandi Xu summarize the collaboration. Xingnuo Cheng and Huandi Xu summarize the safety. Yali Wu summarize the Markov model. Shuyao Zhou and Huandi Xu arrange the website. (Wiki)

10/1/2018-10/9/2018

Kexin Cao, Wanli Zhang, Yunge Lou, Huandi Xu and Junqing Zhang perform MTT and finish the building of plasmids for parts submission. (Experiment) Wanli Zhang identification the efficiency of our promoter. (Experiment) Yali Wu helps Tongji_China with their modeling. Yiyang Zhu designs the animation for booklet. Yunge Lou, Peiyuan Gao and Huandi Xu prepare for the trip to US. Yunge Lou designs the model of liposome gene delivery Shuyao Zhou reviews the texts of collaboration as well as interviewing Pharmaco-Economic experts and Rehabilitation Association of Nanjing CHINA. Xingnuo Cheng translates the paperwork for interviewing Pharmaco-Economic experts and Rehabilitation Association of Nanjing CHINA. Hongyu Wang abstracts the main points of human practice

1 0/10/2018-10/17/2018

Kexin Cao, Wanli Zhang, Yunge Lou, Huandi Xu and Junqing Zhang perform MTT and finish the building of plasmids for parts submission. (Experiment) Wanli Zhang identification the efficiency of our promoter. (Experiment) Yali Wu helps Tongji_China with their modeling. Yiyang Zhu designs the animation for booklet. Yunge Lou, Peiyuan Gao and Huandi Xu prepare for the trip to US. Yunge Lou designs the model of liposome gene delivery Shuyao Zhou reviews the texts of collaboration as well as interviewing Pharmaco-Economic experts and Rehabilitation Association of Nanjing CHINA. Xingnuo Cheng translates the paperwork for interviewing Pharmaco-Economic experts and Rehabilitation Association of Nanjing CHINA. Hongyu Wang abstracts the main points of human practice Wanli Zhang, Kexin Cao perform qPCR, WB and Immuno-fluorescence. (Experiment) Peng Sun, Yali Wu, Xingnuo Cheng and Yunge Lou subtitle the video of interviewing public and Rehabilitation Association of Nanjing CHINA. Shuyao Zhou and Huandi Xu submit the judging form. Junqing Zhang draws the picture for Experiment page (wiki) Huandi Xu and Shuyao Zhou writes paperwork for attribution, human practice summary and education and public engagement.

Before the big freeze !

Yiyang Zhu and Peiyuan Gao built the rest of the wikis. Ying Miao consummates the art designs Yunge Lou and Yali Wu finish the page for modeling. Hongyu Wang reviews the texts for human practice. Huandi Xu designs the drug delivery system. Shuyao Zhou seeks for more application for our system. Kexin Cao and Junqing Zhang write the page for demonstration. Huandi Xu reviews the paperwork for the other parts of wikis.

After iGEM

Shuyao Zhou and Huandi Xu discussed the arrangements for after iGEM. Shuyao Zhou, Yiyang Zhu, Ying Miao and Huandi Xu recomposed and debugged the wiki. Ying Miao and Hongyu Wang applied for reimbursement. Huandi Xu shared the story and experiences with 2019 iGEM team. The whole team shared our thoughts to the public through media on campus. Huandi Xu and Shuyao Zhou arranged the meeting for summary, and the team decided to write a booklet about our experiences.