Team:ETH Zurich/Experiments
Experiments.
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Experiments Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments. Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages. What should this page contain? Protocols Experiments Documentation of the development of your project
Table
Table Subtitle
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Measurement
| LUDOX CL-X | H2O | |
|---|---|---|
| Replicate 1 | 0.063 | 0.034 |
| Replicate 2 | 0.055 | 0.035 |
| Replicate 3 | 0.060 | 0.034 |
| Replicate 4 | 0.056 | 0.035 |
| Arith. Mean | 0.059 | 0.035 |
| Corrected Abs600 | 0.024 | |
| Reference OD600 | 0.063 | |
| OD600/Abs600 | 2.630 |
Particle Standard Curve Log Scale
Particle Standard Curve Linear Scale
Measurement
Sectionh1
Protocoles
Competent Cell Production (from stock)
Introduction
Detergent is a major inhibitor of competent cell growth and transformation. Glass and plastic must be detergent free for these protocols. The easiest way to do this is to avoid washing glassware with detergent, and simply rinse it
out. Autoclaving glassware filled 3/4 with DI water is an effective way to remove most detergent residue. Media and buffers should be prepared in detergent free glassware and cultures grown up in detergent free glassware.
Materials
- CCMB80 buffer
- SOB
- Detergent-free, sterile glassware and plasticware
- Table-top OD600nm spectrophotometer
Procedure
- Prechill 250mL centrifuge tubes and screw cap tubes before use.
Evaluation
Bla Bla
Results
Did not work at all!
Competent Cell Production (from stock)
Introduction
Detergent is a major inhibitor of competent cell growth and transformation. Glass and plastic must be detergent free for these protocols. The easiest way to do this is to avoid washing glassware with detergent, and simply rinse it
out. Autoclaving glassware filled 3/4 with DI water is an effective way to remove most detergent residue. Media and buffers should be prepared in detergent free glassware and cultures grown up in detergent free glassware.
Materials
- CCMB80 buffer
- SOB
- Detergent-free, sterile glassware and plasticware
- Table-top OD600nm spectrophotometer
Procedure
- Prechill 250mL centrifuge tubes and screw cap tubes before use.
Evaluation
Bla Bla
Results
Did not work at all!
