Team:ETH Zurich/WetLab

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Introduction Table
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Measurement
LUDOX CL-X H2O
Replicate 1 0.063 0.034
Replicate 2 0.055 0.035
Replicate 3 0.060 0.034
Replicate 4 0.056 0.035
Arith. Mean 0.059 0.035
Corrected Abs600 0.024  
Reference OD600 0.063  
OD600/Abs600 2.630
Background
Particle Standard Curve Log Scale
Background
Particle Standard Curve Linear Scale
Measurement
Sectionh1
Protocoles
Competent Cell Production (from stock)
Introduction
Detergent is a major inhibitor of competent cell growth and transformation. Glass and plastic must be detergent free for these protocols. The easiest way to do this is to avoid washing glassware with detergent, and simply rinse it out. Autoclaving glassware filled 3/4 with DI water is an effective way to remove most detergent residue. Media and buffers should be prepared in detergent free glassware and cultures grown up in detergent free glassware.
Materials
  • CCMB80 buffer
  • SOB
  • Detergent-free, sterile glassware and plasticware
  • Table-top OD600nm spectrophotometer
Procedure
  1. Prechill 250mL centrifuge tubes and screw cap tubes before use.

Evaluation
Bla Bla
Results
Did not work at all!
Competent Cell Production (from stock)
Introduction
Detergent is a major inhibitor of competent cell growth and transformation. Glass and plastic must be detergent free for these protocols. The easiest way to do this is to avoid washing glassware with detergent, and simply rinse it out. Autoclaving glassware filled 3/4 with DI water is an effective way to remove most detergent residue. Media and buffers should be prepared in detergent free glassware and cultures grown up in detergent free glassware.
Materials
  • CCMB80 buffer
  • SOB
  • Detergent-free, sterile glassware and plasticware
  • Table-top OD600nm spectrophotometer
Procedure
  1. Prechill 250mL centrifuge tubes and screw cap tubes before use.

Evaluation
Bla Bla
Results
Did not work at all!
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