Lane 1: 1kb DNA ladder
Lane 6: Purified PCR product of CBD gBlock
Lane 7: Purified PCR product of BMP2 gBlock
Lane 8: Purified PCR product of sfGFP gBlock
Gel composition
Agarose gel 1%
1X TAE Buffer
Agarose gel 1%
1X TAE Buffer
Lane 1: High Mass DNA ladder
Lane 2: psb1c3-CBD cipA-sfGFP plasmid
Lane 3: psb1c3-CBD cipA-BMP2 plasmid
Agarose gel 1%
1X TAE Buffer
Lane 1: High Mass DNA Ladder
Lane 2-4: Digested product of psb1c3-CBD cipA-BMP2.
Lane 5: Digested product of psb1c3-CBD cipA-sfGFP.
Agarose gel 1%
1X TAE Buffer
Lane 1: 100 pb DNA ladder
Lane 2: PCR ELP product from pET-24a-ELP[V-150] using the primers Forward 1 y Reverse1
Lane 3: PCR ELP product from pET-24a-ELP[V-150] using the primers Forward 1 y Reverse3
Lane 3: PCR ELP product from pET-24a-ELP[V-150] using the primers Forward 1 y Reverse2
Agarose gel 1%
1X TAE Buffer
Lane 1: 100 pb DNA ladder
Lane 2: Purified ELP550
Lane 3: No sample
Lane 3: Unpurified ELP550
Agarose gel 1%
1X TAE Buffer
Lane A: 1 kb DNA ladder
Lane B: BscA 2536 bp
Lane C: BscB 2827 bp
Lane D: empty
Lane E: BscC2 2139 bp
Agarose gel at 1%
1X TAE Buffer
Lane A: 1 kb DNA ladder
Lane B: bscC1 2152 bp
Agarose gel 1%
1X TAE Buffer
Lane A: 1 kb DNA ladder
Lane B: BscA 2536 bp
Lane C: BscB 2827 bp
Lane D: empty
Lane E: BscC2 2139 bp
Agarose gel at 1%
1X TAE Buffer
Lane A: 100 pb DNA ladder
Lane B: unidentified sample
Lane C: BscD 849 pb
Lane D: BscD 849 pb
Agarose gel at 1%
1X TAE Buffer
Lane A: 100 pb DNA ladder
Lane B: lineal Psb1C3 2135 bp
Lane C: lineal Psb1A3 2050 bp
Lane D: bsCmcax 1522 bp
Lane E:bsCcpax (1st attempt) 1512 bp
Lane F:bsCcpax (2nd attempt) 1512 bp
Lane G: bsCcpax (3st attempt) 1512 bp
Lane H: LacI 1420 bp
Agarose Gel 1%
1X TAE Buffer
Lane A: 1 kb DNA ladder
Lane B: gibson assembly psb1C3
Lane C: gibson assembly psb1A3
Using the Gibson assembly kit instructions from NEB we could not see any bands representing the expected ligation product for both plasmids (psb1C3 and psb1A3) (figure 5). A later attempt was done using DMSO, but no change was seen.