Team:Marburg/Part Collection

Part Collection


Figure 1: Hierarchical cloning is facilitated by subsequent Golden Gate reactions.
Basic building blocks like promoters or terminators are stored in level 0 plasmids. Parts from each category of our collection can be chosen to built level 1 plasmids harboring a single transcription unit. Up to five transcription units can be assembled into a level 2 plasmid.
We proudly present the Marburg Collection, a novel golden-gate-based toolbox containing various parts that are compatible with the PhytoBrick system and MoClo. Compared to other bacterial toolboxes, the Marburg Collection shines with superior flexibility. We overcame the rigid paradigm of plasmid construction - thinking in fixed backbone and insert categories - by achieving complete de novo assembly of plasmids. 36 connectors facilitate flexible cloning of multigene constructs and even allow for the inversion of individual transcription units. Additionally, our connectors function as insulators to avoid undesired crosstalk. The Marburg Collection contains 123 parts in total, including: inducible promoters, reporters, fluorescence and epitope tags, oris, resistance cassettes and genome engineering tools. To increase the value of the Marburg Collection, we additionally provide detailed experimental characterization for V. natriegens and a supportive software. We aspire availability of our toolbox for future iGEM teams to empower accelerated progression in their ambitious projects.(Range:BBa_K2560001 - BBa_K2560305)
Figure 2: Additional bases and fusion sites ensure correct spacing and allow tags.
Between some parts, additional base pairs were integrated to ensure correct spacing and to maintain the triplet code. We expanded our toolbox by providing N- and C- terminal tags by creating novel fusions and splitting the CDS and terminator part, respectivel

Parts of the Marburg Collection

Entry Vector
BioBrick Name Plasmid
K2560001 Entry Vector with RFP Dropout BBa_K2560002 (pSB1C3 derivative)
K2560002 Entry Vector with GFP Dropout BBa_K2560002 (pSB1C3 derivative)
K2560005 Resistance Entry Vector with RFP Dropout Falsch
K2560006 Resistance Entry Vector with GFP Dropout Falsch
K2560305 gRNA Entry Vector with GFP Dropout BBa_K2560002 (pSB1C3 derivative)