1. Design of the minimal S. cerevisiae mitochondrial genome. 2. De novo synthesis of the minimal S.
cerevisiae mitochondrial genome. 3. Preparation of Saccharomyces cerevisiae rho0 cells with lost
mitochondrial
genome. 4. Participation and completion of the interlab, and the reception of the data. 5.
Completion of the
construction, characterization and submission of standardized parts 6. Improvement of the
construction,
characterization and submission of the part BBa_K2707013. 7. Knowledge and mastered skills and
operation of
gene
synthesis in TACG Bioscience, TSINGKE and GENERAL BIOSYSTEMS. 8. Participation of iGEM joint meeting
held by
NKU-China, CCIC held by shanghai-tech, and subsequent conduction of in-depth and extensive exchanges
with other
teams, obtaining substantial cooperation. 9. Holding the first iGEM Meetup for universities in
Shaanxi Province 10.
The promotion in primary school and the completion of the activity “DNA Time Capsule” 11. The
assistance in
the
team construction of XJTU-China and the relevant guidance in the competition. 12. Finish of the
preparation of
materials for wiki, poster and presentation.
Members’ Attributions
Lin Weifeng Mainly in charge of the overall operation of the team, also involved in
modeling, the
construction
of
the minimal Saccharomyces cerevisiae mitochondrial genome, interlab, the construction and
characterization of
standardized parts, team communication and cooperation, along with the preparation of Human
practice and
presentation.
Shi Yihong Naming “MitoCRAFT” for the project and engaged with the design of team logo.
Participating in the
construction of the minimal Saccharomyces cerevisiae mitochondrial genome and Human practice.
Li Qingyang Responsible for the construction of the minimal mitochondrial genome.
Liu Qianlong Responsible for part standardization, characterization and improvement, along with collection
and upload of the part data.
Li Meixuan Engaged with the standardization of interlab, part, characterization and data
collection.
Wang Kai Participating in Human practice and data collation, also involved in interlab.
Ma Yuan Responsible for materials and reports writing, finishing and uploading, also
involved in interlab.
Temuer Batu Responsible for the construction and design of team wiki; designing and writing
software for
transcoding text into DNA sequences.
Mi Huiqiao Responsible for art design of our team, including team logo, wiki, poster, PPT
and uniforms.
Li Junyou Engaged with the planning and execution of human practice, the organization and
translation of
materials.
Li Shanshan Responsible for the planning and execution of Human practice; writing and
collating relevant
materials.
General Support
Northwestern Polytechnical University Coming from different schools of Northwestern
Polytechnical University,
NPU-China has received remarkably essential assistance in the entry from NPU.
School of Life Sciences, Northwestern Polytechnical University NPU-China members are mainly
from the School
of
Life Sciences of NPU, which provides important assistance in team building and application for
funding. The
Experimental Center of the school helps provide microplate readers for the completion of
Interlab experiments.
International Cooperation Office, Northwestern Polytechnical University According us with
the registration
fee for the competition and Giant Jamboree, and covering the expenditure for the trip.
Ecological and Environmental Protection Research Center (NPU) Providing experimental lab and
some reagent
support; offering basic molecular biology experimental learning rooms and laboratory safety and
biosafety
training.
Jiang Huifeng, researcher of Tianjin Institute of Industrial Biotechnology, Chinese Academy of
Sciences
(TIIB-CAS) providing theoretical guidance and some experimental hardware and software,
project design and
implementation, HP and other aspects of the project in the preliminary research.
training.
TACG Bioscience Co., Ltd Aiding us in the completion of the synthesis of the first-level
fragments of the
genome, provide the main laboratory support for project experiments, reagent support and some
other financial
support; offering us guidance and help in the splicing technology of macromolecular DNA
fragments; contacting
TSINGKE, GENERAl BIOSYSTEMS for us to carry out the work related to HP.
Project support and advise
Researcher Jiang Huifeng (TIIB-CAS) In the preliminary research process of the project, introducing
for us the
genetic background of mitochondria and the progress of mitochondrial basic research; guiding us to
design the
minimal mitochondrial genome during the genome design process; providing us with the hardware and
software support
needed for some project experiments during the project development; enabling us to access the pMGC
vector
(BBa_K2707007) that was conducive to completing the genome splicing; guiding us to complete the
transformation of
BFD part (BBa_K2707013).
Prof. Qian Airong (NPU): Instructing the team construction and aiding us in the financial support.
Prof. Wang Wen (NPU): Providing the hardware and software support needed for the experiments of the
project.
Prof. Gu Zhenlong (Cornell University): In the preliminary research of the project, introducing for us
the genetic
background of mitochondria and the progress of mitochondrial basic research. In the process of genome
design,
suggesting us to add GFP reporter gene module to the design of the minimal mitochondrial genome;
assisting us in
carrying out the transformation of yeast chassis of the genome (gene gun method) and the verification
of genome
function.
Cheng Jian: Specifically complete the design and modeling of the mitochondrial genome,
along with
the design and codon optimization for the mitochondrial-specific GFP expression cassette.
Ruan Jiangxing: Providing specific guidance on modeling and exploration of pre-experimental conditions
TACG Bioscience Co., Ltd: Helping us complete the synthesis of the first-level fragments of the genome;
providing
software and hardware support for gene combination, and offering us guidance and help on the splicing
technology
of macromolecular DNA fragments.
Researcher Dai Junbiao: According us with the pMGC vector needed for the construction of the genome;
introducing
for us the development history and frontier progress of synthetic genomics on CCiC.
GenScript Biotech Corp.: Helping us mail the official KITS provided by iGEM and our new biological
components to
the committee this year.
Fundraising help and advice
TACG Bioscience Co., Ltd Sponsoring major reagent consumables and experimental funding
support for our
project.
NPU Providing part of the financial support.
International Cooperation Office, Northwestern Polytechnical University Providing us with
the registration
fee for the entry and Giant jamboree; covering the expenditure of the trip.
Yanqi Hanxiao (ICO, NPU) Providing specific assistance in the handling of the reimbursement
procedures of the
International Cooperation Office
Student's Platform for Innovation and Entrepreneurship Training Program (Ministry of education
of the people's
Republic of China) Sponsoring some experimental funding for our project.
Lab Support
Difficult Technique Suport
Researcher Jiang Huifeng (TIIB-CAS) In the preliminary research process of the project, introducing
for us the
genetic background of mitochondria and the progress of mitochondrial basic research; guiding us to
design the
minimal mitochondrial genome during the genome design process; providing us with the hardware and
software support
needed for some project experiments during the project development; enabling us to access the pMGC
vector
(BBa_K2707007) that was conducive to completing the genome splicing; guiding us to complete the
transformation of
BFD part (BBa_K2707013).
Prof. Gu Zhenlong (Cornell University) In the preliminary research of the project, introducing for
us the
genetic background of mitochondria and the progress of mitochondrial basic research. In the process of
genome
design, suggesting us to add GFP reporter gene module to the design of the minimal mitochondrial
genome; assisting
us in carrying out the transformation of yeast chassis of the genome (gene gun method) and the
verification of
genome function.
TACG Bioscience Co., Ltd Helping us complete the synthesis of the first-level fragments of the
genome;
providing software and hardware support for gene combination, and offering us guidance and help on the
splicing
technology of macromolecular DNA fragments.
Researcher Dai Junbiao According us with the pMGC vector needed for the construction of the genome;
introducing
for us the development history and frontier progress of synthetic genomics on CCiC.
Project advisor support
Wiki support
Temuer Batu： In charge of the design and construction of team wiki.
Presentation coaching
Qian Airong： Instructing the contents of presentation
Wang Wen： Instructing A&Q of presentation
Human practice support
Researcher Jiang Huifeng (TIIB-CAS): In the preliminary research process of the project, introducing
for us the
genetic background of mitochondria and the progress of mitochondrial basic research; guiding us to
design the
minimal mitochondrial genome during the genome design process; providing us with the hardware and
software support
needed for some project experiments during the project development; enabling us to access the pMGC
vector
(BBa_K2707007) that was conducive to completing the genome splicing; guiding us to complete the
transformation of
BFD part (BBa_K2707013).
Prof. Gu Zhenlong (Cornell University): In the preliminary research of
the
project,
introducing for us the genetic background of mitochondria and the progress of mitochondrial basic
research. In the
process of genome design, suggesting us to add GFP reporter gene module to the design of the minimal
mitochondrial
genome; assisting us in carrying out the transformation of yeast chassis of the genome (gene gun
method) and the
verification of genome function.
TACG Bioscience Co., Ltd: Helping us complete the synthesis of
the
first-level fragments of the genome; providing software and hardware support for gene combination, and
offering us
guidance and help on the splicing technology of macromolecular DNA fragments.
TSINGKE
Biotechnology
Co.,
Ltd: Zealously welcoming us to visit their company's primer synthesis workshop and gene sequencing
workshop;
introducing us to the industrial process of primer synthesis; elaborating the commercial application of
primer
synthesis and market information.
GENERAl BIOSYSTEMS Co., Ltd: Cordially welcoming us to
visit their
company's primer synthesis workshop, gene synthesis workshop, gene sequencing workshop; introducing us
to the
industrial process of gene synthesis, explaining in detail the commercial application and market
information of
gene synthesis, and making recommendations on gene splicing.
BIOINN Innovation and
Entrepreneurship Center
of Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences: Warmly welcoming us to
visit their
workshops and introducing us to the latest information about the synthetic biology industry and its
trend.
XJTU-China:
Going to elementary school together with us to preach the information about iGEM and synthetic biology;
holding
DNA
Time Capsule activity.
School of Life Sciences, NPU: Providing venues and equipment for the
first iGEM
Meetup in Shaanxi Province
Thanks and acknowledgements for all other people involved in helping make a successful iGEM team>
Hu Xin (Senior, School of Life Sciences, NPU)
Li Qi (Senior, School of Life Sciences, NPU)
Wu Zexun (Senior, School of Life Sciences, NPU)
Team training
Despite that no courses related to iGEM or synthetic biology were carried out, we did conduct comprehensive yet
profound training for our new members, which are listed as follows:
3.17
Conducting interviews and recruiting of new members.
3.22
Starting to learn molecular biology and basic knowledge of synthetic biology; visiting the laboratory
3.31
Learning the contents and format of the 2018 iGEM competition and finding out the deadlines for each
project.
4.08
Getting familiarized with previous years’ iGEM award criteria and laboratory safety training.
4.09-4.15
Learning 2017 wiki of NKU-China and the basic components of an experimental project.
4.16-4.30
Learning 2017 wiki of SZU-China and the basic components of an iGEM entry project; learning basic
experiments on molecular biology.
5.05-5.20
Learning 2017 wiki of PKU-China, getting to know the design and production of genetic circuits, and holding
seminars to allow players to communicate with each other
5.21-6.02
Dipping into the 2016 project of imperial college, opening up perspectives for new projects; team members
making PPTs and communicating at seminars
6.03-6.18
Understanding the 2017 project of Heidelberg, rendering players aware that iGEM projects are not just about
experiments, but also a combination of cutting-edge technologies, such as AI.
Project start
2018.4.2
Project research and brainstorming.
2018.5.2
Initially setting three plans; entering the laboratory for pre-experiment.
2018.6.1
Affirming the project to design and synthesize the minima Saccharomyces cerevisiae mitochondrial genome, and
starting to experiment.
