Team:NPU-China/InterLab

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We're glad to participate the fifth Interlab study with many other teams around the world. This year, the aim of Interlab study is to find out whether we can reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units instead of OD. We are happy that measurement community for accepting our data. It's an interesting and valuable experience to do this work

1. Unit calibration measurements

1.1 Measurement of LUDOX CL-X OD600 reference point

Table 1.OD600 reference point

1.2 Graphing particle standard curve

Figure 1. A standard curve generated by measuring the Abs600 of serial Microsphere suspension dilutions

Figure 2. A standard curve generated by measuring the Abs600 of serial Microsphere suspension dilutions

1.3 Graphing fluorescence standard curve

Figure 3. A standard curve generated by measuring the fluorescence of serial fluorescein dilutions

Figure 4. A standard curve generated by measuring the fluorescence of serial fluorescein dilutions

2. Cell measurement

2.1 Transformation of 8 test devices into competent DH5α cells

The transformation was successful. There was a sufficient amount of colonies on plates except device 4 , which only grew two colonies. We picked 2 colonies from each plates and inoculate in LB medium + chloramphenicol overnight at 37°C and 220 rpm.

2.2 Data of fluorescence from plate reader

Table 2. The fluorescence of six text devices, positive control and negative control in 0 hours and 6 hours

2.3 Data of Abs600 form plate reader

Table 3. The Abs600 of six text devices, positive control and negative control in 0 hour and 6 hour

2.4 Fluorescein per OD

Table 4. Unit scaling factors in sheet of Fluorescein per OD

Table 5. The fluorescence of text devices per OD in 0 hour and 6 hour

Table 6. Net fluorescein a.u in 0 hour and 6 hours

Table 7. Net Abs 600 in 0 hour and 6 hours

2.4 Fluorescein per particle

Table 8. Unit Scaling Factor in sheet of Fluorescein per particle

Table 9. MEFL per particle in different time

Table 10. Net fluorescein a.u. in different time

Table 11. Net Abs 600 in different time

3. Measurement colony forming units per 0.1 OD600 E. coli cultures

Table 12 The number of colonies after diluting and CFU of serial dilution