Team:NPU-China/Part Collection

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This year, NPU-China team focus on building a S.cerevisiae mitochondrial genome part collection. We submitted all the proteins expressed in the mitochondrial genome of S.cerevisiae, we hope to help teams that is engaged in related research in the future.

Name	Type	Description	Designer	length
BBa_K2707000	Coding	atp9	Weifeng Lin	231
BBa_K2707001	coding	cox1	Weifeng Lin	1605
BBa_K2707002	coding	cox2	Weifeng Lin	756
BBa_K2707003	coding	cox3	Weifeng Lin	810
BBa_K2707004	coding	cob	Weifeng Lin	1158
BBa_K2707005	coding	atp8	Weifeng Lin	147
BBa_K2707006	coding	atp6	Weifeng Lin	780
BBa_K2707008	coding	var1	Weifeng Lin	1197

cob (BBa_K2707004)
cob encode cytochrome b in mitochondrion.
Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c. Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis.

var1 (BBa_K2707008)
var1 encode ribosomal protein in mitochondrion.
Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane . uS3m is essential for mitochondrial protein synthesis and required for the maturation of small ribosomal subunits .

cox1 (BBa_K2707001) cox2 (BBa_K2707002) cox3 (BBa_K2707003)
cox1 cox2 cox3 encode the components of Cytochrome c oxidase in mitochondrion
Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water.
Catalytic activity:
4 ferrocytochrome c + O₂ + 4 H⁺ = 4 ferricytochrome c + 2 H₂O.
Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
Pathway : oxidative phosphorylation

atp6 (BBa_K2707006) atp8 (BBa_K2707005) atp9 (BBa_K2707000)
atp6 atp8 atp9 encode 3 subunits of ATPase respectively.
Mitochondria membrane ATP synthase (F₁F₀ ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F₁ - containing the extramembraneous catalytic core and F₀ - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F₁ is coupled via a rotary mechanism of the central stalk subunits to proton translocation. As the key component of the proton channel; atp6 may play a direct role in the translocation of protons across the membrane. As atp8 is the part of the complex F₀ domain, minor subunit located with subunit a in the membrane. As atp9 is the part of the complex F₀ domain, a homomeric c-ring of probably 10 subunits is part of the complex rotary element.