Difference between revisions of "Team:WashU StLouis/Daily"

Line 1,565: Line 1,565:
 
     <p><em>Present: Cam, Elizabeth, Havisha, Kyle</em></p>
 
     <p><em>Present: Cam, Elizabeth, Havisha, Kyle</em></p>
 
     <ul>
 
     <ul>
−
         <li>ploop</li>
+
         <li>Met with Dr. Shah from the Danforth Plant Science Center [Cam, Elizabeth, Havisha, Kyle]</li>
 +
        <li>Transformed <I>DH5α</I> cells for Day 1 of the interlab protocol [Cam, Elizabeth, Havisha, Kyle]</li>
 +
        <li>Transformed electrocompetent <I>DH5α</I> cells for practice [Cam, Elizabeth, Havisha, Kyle]</li>
 
     </ul>
 
     </ul>
 
     </div>
 
     </div>
Line 1,576: Line 1,578:
 
     <p><em>Present: Cam, Elizabeth, Kyle</em></p>
 
     <p><em>Present: Cam, Elizabeth, Kyle</em></p>
 
     <ul>
 
     <ul>
−
         <li>Attended the iGEM Midwest Meet Up at the University of Illinois Urbana-Champaign [Cam, Elizabeth, Kyle]</li>
+
         <li>Attended the iGEM Midwest Meetup [Cam, Elizabeth]</li>
 +
        <li>Started overnight cultures for Day 2 of the interlab protocol [Kyle]</li>
 
     </ul>
 
     </ul>
 
     </div>
 
     </div>
Line 1,587: Line 1,590:
 
     <p><em>Present: Cam, Elizabeth, Kyle</em></p>
 
     <p><em>Present: Cam, Elizabeth, Kyle</em></p>
 
     <ul>
 
     <ul>
−
         <li>ploop</li>
+
         <li>Started overnight cultures for Day 2 of the interlab protocol [Elizabeth]</li>
 +
        <li>Transformed <I>DH5α</I> cells with positive control, negative control, and test device two for Day 1 of the interlab protocol [Cam, Elizabeth, Kyle]</li>
 
     </ul>
 
     </ul>
 
     </div>
 
     </div>
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     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><strong><u>Sunday, August 19</u></strong></p>
 
     <p><strong><u>Sunday, August 19</u></strong></p>
−
     <p><em>Present: Cam, Elizabeth, Kyle</em></p>
+
     <p><em>Present: Cam, Elizabeth</em></p>
 
     <ul>
 
     <ul>
−
         <li>ploop</li>
+
         <li>Made overnight cultures of positive control, negative control, and test device two for Day 2 of the interlab protocol [Cam]</li>
 +
        <li>Performed part three of the interlab calibration protocol [Elizabeth]</li>
 +
        <li>Made LB agar and chloramphenicol plates [Elizabeth]</li>
 
     </ul>
 
     </ul>
 
     </div>
 
     </div>
Line 1,609: Line 1,615:
 
     <p><em>Present: Cam, Elizabeth, Kyle</em></p>
 
     <p><em>Present: Cam, Elizabeth, Kyle</em></p>
 
     <ul>
 
     <ul>
−
         <li>ploop</li>
+
         <li>Finalized sequencing primers [Elizabeth]</li>
 +
        <li>Transformed <I>DH5α</I> cells with positive control, test device one, test device two, and test device three for Day 1 of the interlab protocol [Cam, Elizabeth, Kyle]</li>
 +
        <li>Made LB agar and chloramphenicol plates [Cam, Elizabeth]</li>
 +
        <li>Transformed <I>DH5α</I> cells with YFP [Kyle]</li>
 
     </ul>
 
     </ul>
 
     </div>
 
     </div>
Line 1,620: Line 1,629:
 
     <p><em>Present: Cam, Elizabeth, Kyle</em></p>
 
     <p><em>Present: Cam, Elizabeth, Kyle</em></p>
 
     <ul>
 
     <ul>
−
         <li>ploop</li>
+
         <li>Transformed DH5α cells with Gal1, Tef2, and RFP + terminator [Cam, Elizabeth, Kyle]</li>
 
     </ul>
 
     </ul>
 
     </div>
 
     </div>
Line 1,629: Line 1,638:
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><strong><u>Wednesday, August 22</u></strong></p>
 
     <p><strong><u>Wednesday, August 22</u></strong></p>
−
     <p><em>Present: Cam, Elizabeth, Kyle</em></p>
+
     <p><em>Present: Cam, Kyle</em></p>
 
     <ul>
 
     <ul>
−
         <li>ploop</li>
+
         <li>Autoclaved water and culture tubes [Cam, Kyle]</li>
 +
        <li>Made overnight cultures of Gal1, Tef2, and RFP + terminator [Cam]</li>
 
     </ul>
 
     </ul>
 
     </div>
 
     </div>
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     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><strong><u>Thursday, August 23</u></strong></p>
 
     <p><strong><u>Thursday, August 23</u></strong></p>
−
     <p><em>Present: Cam, Elizabeth, Kyle</em></p>
+
     <p><em>Present: Cam</em></p>
 
     <ul>
 
     <ul>
−
         <li>ploop</li>
+
         <li>Made overnight cultures of for Day 2 of the interlab protocol [Cam]</li>
 
     </ul>
 
     </ul>
 
     </div>
 
     </div>
Line 1,651: Line 1,661:
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><strong><u>Friday, August 24</u></strong></p>
 
     <p><strong><u>Friday, August 24</u></strong></p>
−
     <p><em>Present: </em></p>
+
     <p><em>Present: Elizabeth, Havisha, Kyle</em></p>
 
     <ul>
 
     <ul>
−
         <li>ploop</li>
+
         <li>Performed hour zero preparations for Interlab Day 3 [Havisha]</li>
 +
        <li>Performed hour six for Interlab Day 3 [Elizabeth]</li>
 +
        <li>Set up digestion reactions for Sr35 part 1, Sr35 part 2, GAL1, and TEF1 [Elizabeth]</li>
 +
        <li>Ran gel with Sr35 part 1, Sr35 part 2, GAL1, and TEF1 [Elizabeth, Havisha]</li>
 +
        <li>Performed PCR for AvrSr35 [Havisha]</li>
 +
        <li>Autoclaved tips [Kyle]</li>
 
     </ul>
 
     </ul>
 
     </div>
 
     </div>

Revision as of 04:45, 25 August 2018

June 2018
Sunday Monday Tuesday Wednesday Thursday Friday Saturday
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30

Friday, June 1

No work done today!

Saturday, June 2

No work done today!

Sunday, June 3

No work done today!

Monday, June 4

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Researched different resistance genes to identify ones that are sequenced and applicable to the project [Elizabeth, Diva, Cam]
  • Studied glucosamine detection mechanisms to signal the presence the presence of fungi [Havisha]
  • Planned trip to Uganda and human practices potential abroad [Kyle]

Tuesday, June 5

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Researched different resistance genes to identify ones that are sequenced and applicable to the project [Elizabeth, Diva]
  • Compared different aspects of Sr genes [Cam]
  • Examined a protocol for rapid cloning of plant disease resistance genes [Diva]
  • Studied glucosamine detection mechanisms to signal the presence the presence of fungi [Havisha, Diva]
  • Planned trip to Uganda and potential human practices abroad [Kyle]

Wednesday, June 6

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Examined lengths of resistance gene sequences to begin ordering material [Elizabeth]
  • Studied fungal spore traps to limit specificity to Puccinia graminis [Havisha]
  • Investigated different detection and reporting mechanisms for reporting information with a device [Cam, Diva]
  • Planned trip to Uganda and potential human practices abroad [Kyle]

Thursday, June 7

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Met with Dr. Brennan to redefine project goals and timeline [Cam, Diva, Elizabeth, Havisha, Kyle]
  • Reviewed videos from the BGRI to determine locations of wheat rust fungus prevalence [Elizabeth]
  • Prepared presentation for Microsoft partnership meeting and consulted Dr. Brennan on areas for improvement [Cam]
  • Studied fungal spore traps to limit specificity to Puccinia graminis [Havisha]
  • Continued research on various detection mechanisms [Diva]
  • Planned trip to Uganda and potential human practices abroad [Kyle]

Friday, June 8

Present: Cam, Diva, Elizabeth, Havisha

  • Met with a representative from Microsoft to establish a technology partnership [Cam]
  • Reviewed papers regarding resistance gene interactions (Sr35, Sr22, and Sr45) [Elizabeth]
  • Considered phage related detection mechanisms, alongside the use of CRISPR/CAS9 [Diva]
  • Studied fungal spore traps to select only ones of Puccinia graminis [Havisha]

Saturday, June 9

No work done today!

Sunday, June 10

No work done today!

Monday, June 11

Present: Cam, Diva, Elizabeth, Havisha

  • Prepared a summary of the global impact of rust for Microsoft [Cam]
  • Planned for the use of Sr33 and Sr13 [Elizabeth]
  • Studied compounds unique to germinated P. graminis and infectious structures [Havisha]
  • Investigated the DNA repair mechanism of eukaryotes (fungi in particular) [Diva]

Tuesday, June 12

Present: Cam, Diva, Elizabeth, Havisha

  • Revised our project abstract to incorporate changes made over the past week [Cam, Diva, Elizabeth, Havisha]
  • Conducted a marketing brainstorming session [Cam, Diva, Elizabeth, Havisha]
  • Researched Sr50 and truncating resistance genes [Elizabeth]
  • Compiled a research summary for Microsoft [Cam]
  • Examined different detection mechanisms for ribitol [Havisha]
  • Investigated the DNA repair mechanism of eukaryotes (fungi in particular) [Diva]

Wednesday, June 13

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Met with Dr. Brennan to assess abstract and project design in preparation to meet with Monsanto [Cam, Diva, Elizabeth, Kyle, Havisha]
  • Compiled gene sequences needed for the project [Elizabeth]
  • Began developing the wiki [Havisha]
  • Identified the sequence for a gene induced by ribotin to transfer into a plasmid and transform E. coli with [Havisha]
  • Investigated the DNA repair mechanism of eukaryotes (fungi in particular) [Diva]
  • Planned trip to Uganda and potential human practices abroad [Kyle]

Thursday, June 14

Present: Cam, Diva, Elizabeth, Havisha

  • Connected with many iGEM teams through social media [Elizabeth, Cam]
  • Developed the wiki template and daily notebook log pages [Havisha]
  • Attempted to identify promoter sequences induced by ribotin [Diva]
  • Investigated truncating Sr50 and fusion proteins [Elizabeth]

Friday, June 15

Present: Cam, Diva, Elizabeth, Havisha

  • Developed footer and human practices page of the wiki [Havisha]
  • Conducted a call with a grain farmer for fundraising and human practices efforts [Cam]
  • Organized presentation in preparation for a meeting with Monsanto [Cam]
  • Investigated Sr35 and fusion proteins [Elizabeth]
  • Met with Jeff to discuss logistics of project [Havisha, Elizabeth]

Saturday, June 16

No work done today!

Sunday, June 17

No work done today!

Monday, June 18

Present: Cam, Diva, Elizabeth, Havisha

  • Worked on presentation in preparation for meeting with Monsanto [Cam, Diva, Elizabeth, Havisha]
  • Prepared PBS buffer and LB Agar plates in preparation for interlab [Cam, Diva, Elizabeth, Havisha]
  • Developed technology partnership with Microsoft. [Cam]

Tuesday, June 19

Present: Cam, Diva, Elizabeth, Havisha

  • Met with Dr. Brennan to prepare for Monsanto presentation. [Cam, Diva, Elizabeth, Havisha]
  • Prepared LB buffer and began overnight culture. [Cam, Diva, Elizabeth, Havisha]

Control agar + CM plates made on 6/18

Wednesday, June 20

Present: Cam, Diva, Elizabeth, Havisha

  • Prepared chemically competent DH5α E. coli cells [Cam, Diva, Elizabeth, Havisha]
  • Developed list of protocols for the wiki [Havisha, Diva]

Thursday, June 21

Present: Cam, Diva, Elizabeth, Havisha

  • Toured Monsanto and Pfizer facilities [Cam, Diva, Elizabeth, Havisha]
  • Presented to Monsanto researchers to solicit advice and feedback from people in the field [Cam, Diva, Elizabeth, Havisha]

Friday, June 22

Present: Cam, Diva, Elizabeth, Havisha

  • Identified gene sequences for ribitol metabolism [Havisha]
  • Began investigating LAMP and primers [Diva]
  • Studied protein-protein interactions of CC-NLR proteins [Diva, Elizabeth]
  • Continued with fundraising efforts and coordinating partnership with Monsanto [Cam]

Saturday, June 23

No work done today!

Sunday, June 24

No work done today!

Monday, June 25

Present: Cam, Diva, Havisha

  • Worked on Day 1 of the interlab study protocol [Cam, Diva, Havisha]

Tuesday, June 26

Present: Cam, Diva, Havisha

  • Troubleshooted problems with Day 1 transformation [Cam, Diva, Havisha]
  • Corresponded with Microsoft regarding digital lab notebook [Cam]
  • Solidified gene sequences needed for general detection mechanism [Havisha]
  • Began filling out iGEM safety form part 1 [Diva]

Transformed DH5α from interlab studies day one on 6/25

Wednesday, June 27

Present: Cam, Diva, Havisha

  • Wrote project overview, background, and general detection mechanism summary for wiki [Havisha]
  • Completed safety form [Diva, Havisha]
  • Made LB Agar Media [Diva, Havisha]
  • Started an overnight culture of DH5α [Diva, Havisha]

Thursday, June 28

Present: Diva, Elizabeth, Havisha

  • Submitted safety form [Diva]
  • Plated LB Agar plates [Diva, Elizabeth, Havisha]
  • Made chemically competent DH5α [Diva, Elizabeth, Havisha]
  • Developed banners for wiki pages [Havisha]

Friday, June 29

Present: Diva, Elizabeth, Havisha

  • Worked on members’ introductions for the wiki [Diva, Elizabeth, Havisha]
  • Tested competency of cells prepared on 6/28 [Diva, Elizabeth, Havisha]

Control agar plate made on 6/28

Saturday, June 30

No work done today!

Sunday, July 1

Present: Diva, Havisha

  • Performed calibration protocol for interlab study [Diva, Havisha]

Control agar plate with non-transformed competent cells plated on 6/29

Agar + CM plates with cells transformed with iGEM test kit from 6/29

Monday, July 2

Present: Diva, Elizabeth, Havisha

  • Made LB Agar + CM plates [Diva, Elizabeth, Havisha]
  • Transformed E. coli DH5α for Interlab Protocol Day 1 [Diva, Elizabeth, Havisha]

Tuesday, July 3

Present: Diva, Elizabeth, Havisha

  • Met with Jeff to discuss project goals and progress [Diva, Elizabeth, Havisha]

Wednesday, July 4

Present: Cam, Diva, Elizabeth

  • Worked on Day 2 of interlab protocol [Cam, Diva, Elizabeth]

Thursday, July 5

Present: Cam, Diva, Elizabeth

  • Worked on Day 3 of interlab protocol [Cam, Diva, Elizabeth]
  • Informed of contamination with E. coli and materials [Cam, Diva, Elizabeth]

Friday, July 6

Present: Cam, Diva, Elizabeth, Kyle

  • Made 1L LB Broth [Diva]
  • Made LB Agar + CM plates [Cam, Elizabeth]
  • Made TSS Buffer [Elizabeth, Diva]
  • Looked into GPCRs [Kyle]

Saturday, July 7

Present: Cam, Elizabeth

  • Transformed DH5α cells using competent cell test kit [Cam, Elizabeth]

Sunday, July 8

Present: Elizabeth

  • Checked transformed cell plates for growth [Elizabeth]

Agar + CM plates with cells transformed with iGEM test kit from 7/7

Monday, July 9

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Consulted with Microsoft computing experts on modelling and device [Cam]
  • Finalized ribitol operon sequences to order [Havisha]
  • Studied resistance genes and sequences to order [Elizabeth]

Tuesday, July 10

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Met with Dr. Brennan to discuss project checkpoints and progress [Elizabeth, Havisha, Kyle]
  • Made new LB media [Diva]
  • Made and plated LB agar and LB agar + CM [Diva, Elizabeth, Havisha]
  • Studied resistance genes and sequences to order [Elizabeth]
  • Ordered ribitol operon gene sequences [Havisha]
  • Met with Dr. Parikh and attended a lecture to develop international human practices [Kyle]
  • Began DH5α overnight culture [Diva, Elizabeth, Havisha]

Wednesday, July 11

Present: Diva, Elizabeth, Havisha

  • Plated DH5α cells on LB agar plate to check for plate contamination [Havisha]
  • Checked plated DH5α cells [Diva, Elizabeth]

Agar plate with overnight culture of DH5α from 7/11

Thursday, July 12

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Conducted Day 1 of interlab protocol with competent cells from NEB [Cam, Diva, Elizabeth, Havisha, Kyle]
  • Discussed human practices progress and integration into project [Cam, Diva, Elizabeth, Havisha, Kyle]
  • Checked plated transformed DH5α cells [Diva, Elizabeth]

Agar + CM plates with transformed cells from Day 1 of interlab protocol from 7/12

Friday, July 13

Present: Cam, Diva, Elizabeth, Kyle

  • Conducted Day 2 of interlab protocol [Diva, Elizabeth]
  • Met with Monsanto plant scientists to discuss project design and resistance genes [Diva, Elizabeth, Kyle]
  • Conducted Hour 0 of Day 3 of interlab protocol [Cam, Diva, Elizabeth]
  • Made LB Agar + CM plates [Cam, Diva]

Saturday, July 14

Present: Cam, Diva, Elizabeth, Kyle

  • Conducted Hour 6 of Day 3 of interlab protocol [Diva, Elizabeth, Kyle]
  • Made LB broth [Cam]

Sunday, July 15

No work done today!

Monday, July 16

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Conducted a production meeting to redefine project progress and delegate individual tasks [Cam, Diva, Elizabeth, Havisha, Kyle]
  • Compiling gene sequences for resistance genes [Elizabeth]
  • Met with a representative from the Missouri Coalition for the Environment [Kyle]
  • Worked on device logistics and reached out to possible advisors for development [Kyle]
  • Procured space in WashU makerspace for device prototyping [Cam]
  • Solidified wiki homepage formatting [Havisha]
  • Looked into longevity of E. coli and yeast and possible alternatives to maintaining a live culture in device [Diva]

Tuesday, July 17

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Researched biobrick scars and Gibson assembly as a possible alternative [Elizabeth]
  • Finalized gene sequences for resistance genes [Elizabeth]
  • Began developing poster for EECE interns presentation [Havisha]
  • Ordered ribitol for testing transformed cells [Cam]
  • Contacted Bayer for possible collaborations with education and human practices [Kyle]
  • Worked on device design [Kyle]
  • Explored powdered cells for one time use as an alternative to a live culture in device [Diva]

Wednesday, July 18

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Worked on poster for presentation [Cam, Diva, Elizabeth, Havisha, Kyle]
  • Worked on summary presentation for video conference [Cam, Diva, Elizabeth, Havisha, Kyle]
  • Looked into plasmids and promoter to use with ribitol operon [Havisha]
  • Ordered AvrSr35 gene sequences [Elizabeth]

Thursday, July 19

Present: Cam, Elizabeth, Havisha, Kyle

  • Attended and presented at Southern iGEM video conference [Elizabeth, Havisha, Kyle]
  • Made Agar + CM plates [Cam, Havisha, Kyle]
  • Discussed and consulted grad students regarding golden gate assembly for resistance gene sequences [Cam, Elizabeth, Havisha, Kyle]
  • Worked on poster for presentation [Havisha]

Friday, July 20

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Met with the head of St. Louis Indoor Produce and toured facility for possible collaborations and human practices [Diva, Elizabeth, Havisha, Kyle]
  • Met to discuss and define project timeline and goals [Cam, Diva, Elizabeth, Havisha, Kyle]

Saturday, July 21

Present: Diva, Elizabeth, Havisha, Kyle

  • Conducted a collaborations video call with the ICT Mumbai iGEM team [Elizabeth, Kyle]
  • Attended and presented at North American Kick-off event video conference [Elizabeth, Havisha, Kyle]
  • Transformed DH5α with positive and negative controls for interlab CFU/mL protocol [Diva, Havisha]
  • Checked plates and began four overnight cultures for interlab CFU/mL protocol [Diva]

Agar + CM plates with transformed cells from for CFU interlab protocol from 7/21

Sunday, July 22

Present: Diva, Havisha

  • Conducted dilution and plating steps for interlab CFU/mL protocol [Diva, Havisha]

Monday, July 23

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Conducted a production meeting to redefine project progress and delegate individual tasks [Cam, Diva, Elizabeth, Havisha, Kyle]
  • Met with Dr. Feher to discuss device logistics [Kyle]
  • Counted colonies on plates for interlab CFU/mL protocol [Cam, Diva, Elizabeth, Kyle]
  • Met with Microsoft to touch base on goals for the modeling component of our project [Cam, Diva, Elizabeth, Havisha, Kyle]
  • Met with Dr. Brennan to discuss part assembly and testing of ordered parts [Diva, Elizabeth, Havisha]

Agar + CM plates with diluted cultures of transformed cells from for CFU interlab protocol from 7/22

Tuesday, July 24

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Toured the Danforth Plant Sciences Center [Cam, Elizabeth, Havisha, Kyle]
  • Finished and printed poster [Diva]

Wednesday, July 25

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Presented at EECE Interns Poster Session [Cam, Diva, Elizabeth, Havisha, Kyle]
  • Met with Dr. Kunkel to discuss resistance genes and application of project [Elizabeth, Havisha]
  • Ordered Sr35 genes [Elizabeth]

Thursday, July 26

Present: Cam, Diva, Elizabeth, Kyle

  • Made ampicillin and chloramphenicol [Cam]
  • Made kanamycin [Elizabeth]
  • Made LB+Cam plates [Kyle]
  • Made overnight DH5α cultures [Diva]

Friday, July 27

Present: Diva, Elizabeth, Kyle

  • Made glycerol stocks of DH5α cells [Diva]
  • Made competent DH5α cells [Diva, Elizabeth, Kyle]

Saturday, July 28

Present: Elizabeth, Kyle

  • Ran competent cell test kit [Elizabeth, Kyle]
  • Discussed human practices [Elizabeth, Kyle]

Sunday, July 29

Present: Diva, Elizabeth, Kyle

  • Checked plates from competent cell kit [Elizabeth]
  • Worked on wiki content for global human practices [Diva, Kyle]

Monday, July 30

Present: Diva, Elizabeth, Kyle

  • Grew an overnight culture of DH5α cells [Diva]
  • Transformed NEB competent DH5α cells with pSB1C3 (pre-miniprep) [Elizabeth]
  • Tested for competent cells [Kyle]
  • Met with Dr. Brennan to discuss part assembly and competent cell preparation protocol [Diva, Elizabeth, Kyle]
  • Met with Jeff and other grad students to discuss preventing contamination and improving efficacy of interlab technique [Diva, Elizabeth, Kyle]

Tuesday, July 31

Present: Diva, Elizabeth, Kyle

  • Made competent DH5α cells [Kyle, Elizabeth]
  • Tested TSS and SOC media pH with glass pH probe [Elizabeth, Diva]

Cells transformed with pSB1C3 from 7/30

Results from competent cell test kit from 7/30

Wednesday, August 1

Present: Diva

  • Made LB agar plates [Diva]

Thursday, August 2

Present: Cam, Diva, Elizabeth, Kyle

  • Made competent DH5α cells [Cam, Diva]
  • Transformed competent cells with RFP [Kyle, Elizabeth]

Friday, August 3

Present: Cam, Diva, Elizabeth, Kyle

  • Transformed competent cells with Part BBa_K2663000 [Elizabeth, Kyle]
  • Made LB agar + CM plates [Diva, Cam]
  • Made overnight cultures of cells transformed with RFP [Cam]

Saturday, August 4

Present: Elizabeth, Kyle

  • Made LB [Elizabeth, Kyle]
  • Made glycerol stocks of overnight cultures [Elizabeth, Kyle]
  • Plated cells to check for contamination [Elizabeth, Kyle]
  • Ordered miniprep kit [Elizabeth, Kyle]
  • Checked plates to ensure lack of contamination [Elizabeth, Kyle]
  • Plated overnight cultures [Elizabeth, Kyle]

Sunday, August 5

Present: Elizabeth

  • Made glycerol stocks from overnight cultures [Elizabeth]
  • Miniprepped RFP, ribitol component promoter, and RBS [Elizabeth]
  • Autoclaved new tips and microcentrifuge tubes [Elizabeth]
  • Made a negative control to test for contamination in the culture tubes [Elizabeth]

Monday, August 6

Present: Cam, Diva, Elizabeth, Kyle

  • Made ampicillin plates [Cam, Diva]
  • Measured DNA concentrations using nanodrop [Cam, Diva, Elizabeth, Kyle]
  • Made more ampicillin, chloramphenicol, and kanamycin [Cam, Diva]
  • Plated pRSII424 on ampicillin plate [Cam, Diva]

Tuesday, August 7

Present: Cam, Diva, Elizabeth, Kyle

  • Made an overnight culture of pRSII424 [Cam, Elizabeth, Kyle]

Wednesday, August 8

Present: Elizabeth, Havisha, Kyle

  • Minipreped pRSII424 plasmid [Elizabeth, Havisha, Kyle]
  • Performed PCR on ribitol operon, ribitol transporter, and AvrSr35 [Elizabeth, Havisha, Kyle]
  • Began an overnight culture of DH5α cells [Elizabeth, Havisha]

Thursday, August 9

Present: Cam, Havisha, Kyle

  • Conducted collaboration video calls with Cardiff, Westminster, and University of Minnesota iGEM teams [Havisha, Kyle]
  • Made competent DH5α cells [Cam]
  • Performed PCR on yeast plasmid backbone [Havisha]
  • Made and ran gel electrophoresis with PCR products [Havisha]

Friday, August 10

Present: Elizabeth, Havisha

  • Performed PCR on ribitol operon, ribitol transporter, and AvrSr35 [Havisha]
  • Made and ran gel electrophoresis with PCR products [Havisha]
  • Transformed DH5α cells with mini prepped pSB1C3 plasmid with RFP [Elizabeth]
  • Transformed DH5α cells with YFP Part BBa_I6031[Elizabeth]

Saturday, August 11

Present: Cam, Havisha

  • Purified ribitol transporter and AvrSr35 from agarose gel [Cam, Havisha]
  • Performed PCR on ribitol operon, ribitol transporter, and pRSII424 plasmid [Cam, Havisha]
  • Made and ran gel electrophoresis with PCR products [Cam, Havisha]

Cells transformed with pSB1C3 from 8/10

Sunday, August 12

Present: Cam, Havisha

  • Performed PCR on ribitol operon and ribitol transporter [Havisha]
  • Made SOC Media [Cam]
  • Purified pRSII424 plasmid from agarose gel [Cam, Havisha]
  • Transformed DH5α cells with pSB1A3 plasmid [Cam, Havisha]
  • Transformed DH5α cells with pSB1K3 plasmid [Cam, Havisha]
  • Transformed DH5α cells with YFP Part BBa_I6031 [Cam, Havisha]

Monday, August 13

Present: Cam, Havisha

  • Began an overnight culture of cells transformed with pSB1A3 plasmid [Havisha]
  • Made LB agar + Kanamycin plates [Cam, Havisha]
  • Transformed DH5α cells with pSB1K3 plasmid [Cam, Havisha]
  • Transformed DH5α cells with YFP Part BBa_I6031 [Cam, Havisha]

Cells transformed with pSB1A3 from 8/12

Cells transformed with YFP from 8/12

Tuesday, August 14

Present: Cam, Havisha

  • Made LB + Amp Media [Cam, Havisha]
  • Made LB + Kan Media [Cam, Havisha]
  • Began an overnight culture of DH5α cells with pSB1K3 plasmid [Cam, Havisha]
  • Began an overnight culture of DH5α cells with YFP Part BBa_I6031 [Cam, Havisha]
  • Began an overnight culture of DH5α cells with pSB1A3 plasmid [Cam, Havisha]

Cells transformed with pSB1K3 from 8/13

Cells transformed with YFP from 8/13

Wednesday, August 15

Present: Cam, Elizabeth, Havisha, Kyle

  • Miniprepped pSB1K3 plasmid [Cam, Elizabeth, Havisha]
  • Miniprepped YFP Part BBa_I6031 [Cam, Elizabeth, Havisha]
  • Miniprepped pSB1A3 plasmid [Cam, Elizabeth, Havisha]
  • Worked on arduino coding for device [Kyle]
  • Transformed electrocompetent DH5α cells for practice [Cam, Elizabeth, Havisha, Kyle]

Overnight cultures of YFP, pSB1A3 plasmid, and pSB1K3 plasmid from 8/14

Thursday, August 16

Present: Cam, Elizabeth, Havisha, Kyle

  • Met with Dr. Shah from the Danforth Plant Science Center [Cam, Elizabeth, Havisha, Kyle]
  • Transformed DH5α cells for Day 1 of the interlab protocol [Cam, Elizabeth, Havisha, Kyle]
  • Transformed electrocompetent DH5α cells for practice [Cam, Elizabeth, Havisha, Kyle]

Friday, August 17

Present: Cam, Elizabeth, Kyle

  • Attended the iGEM Midwest Meetup [Cam, Elizabeth]
  • Started overnight cultures for Day 2 of the interlab protocol [Kyle]

Saturday, August 18

Present: Cam, Elizabeth, Kyle

  • Started overnight cultures for Day 2 of the interlab protocol [Elizabeth]
  • Transformed DH5α cells with positive control, negative control, and test device two for Day 1 of the interlab protocol [Cam, Elizabeth, Kyle]

Sunday, August 19

Present: Cam, Elizabeth

  • Made overnight cultures of positive control, negative control, and test device two for Day 2 of the interlab protocol [Cam]
  • Performed part three of the interlab calibration protocol [Elizabeth]
  • Made LB agar and chloramphenicol plates [Elizabeth]

Monday, August 20

Present: Cam, Elizabeth, Kyle

  • Finalized sequencing primers [Elizabeth]
  • Transformed DH5α cells with positive control, test device one, test device two, and test device three for Day 1 of the interlab protocol [Cam, Elizabeth, Kyle]
  • Made LB agar and chloramphenicol plates [Cam, Elizabeth]
  • Transformed DH5α cells with YFP [Kyle]

Tuesday, August 21

Present: Cam, Elizabeth, Kyle

  • Transformed DH5α cells with Gal1, Tef2, and RFP + terminator [Cam, Elizabeth, Kyle]

Wednesday, August 22

Present: Cam, Kyle

  • Autoclaved water and culture tubes [Cam, Kyle]
  • Made overnight cultures of Gal1, Tef2, and RFP + terminator [Cam]

Thursday, August 23

Present: Cam

  • Made overnight cultures of for Day 2 of the interlab protocol [Cam]

Friday, August 24

Present: Elizabeth, Havisha, Kyle

  • Performed hour zero preparations for Interlab Day 3 [Havisha]
  • Performed hour six for Interlab Day 3 [Elizabeth]
  • Set up digestion reactions for Sr35 part 1, Sr35 part 2, GAL1, and TEF1 [Elizabeth]
  • Ran gel with Sr35 part 1, Sr35 part 2, GAL1, and TEF1 [Elizabeth, Havisha]
  • Performed PCR for AvrSr35 [Havisha]
  • Autoclaved tips [Kyle]

Saturday, August 25

Present:

  • ploop

Sunday, August 26

Present:

  • ploop

Monday, August 27

No work done today!

Tuesday, August 28

No work done today!

Wednesday, August 29

No work done today!

Thursday, August 30

No work done today!

Friday, August 31

Present: Collin

Lab Work

  1. Prepared overnight cultures from glycerol stock of lac+RBS+BCP+pSB1C3 [Collin]

Outside Work / Discussion

Saturday, September 1

No work done today!

Sunday, September 2

No work done today!

Monday, September 3

No work done today!

Tuesday, September 4

No work done today!

Wednesday, September 5

No work done today!

Thursday, September 6

No work done today!

Friday, September 7

No work done today!

Saturday, September 8

No work done today!

Sunday, September 9

No work done today!

Monday, September 10

No work done today!

Tuesday, September 11

No work done today!

Wednesday, September 12

No work done today!

Thursday, September 13

No work done today!

Friday, September 14

Present:Mark

Lab Work

  1. Transformed Lac+RBS+BCP+pSB1C3 into E.coli cells [Mark]

Outside Work / Discussion

Saturday, September 15

No work done today!

Monday, September 16

Present:Maddie

Lab Work

  1. Started a cyanobacteria culture [Maddie]
  2. Performed GoldenBraid PCR for uvsE, phrAC, phrAT, and Dsup [Maddie]

Outside Work / Discussion

Monday, September 17

No work done today!

Tuesday, September 18

No work done today!

Wednesday, September 19

No work done today!

Thursday, September 20

No work done today!

Friday, September 21

No work done today!

Saturday, September 22

No work done today!

Sunday, September 23

No work done today!

Monday, September 24

No work done today!

Tuesday, September 25

No work done today!

Wednesday, September 26

No work done today!

Thursday, September 27

No work done today!

Friday, September 28

Present: Collin

Lab Work

  1. Refreshed Cyanobacteria culture [Collin]

Outside Work / Discussion

Saturday, September 29

No work done today!

Sunday, September 30

Present:Collin, Micah, Mark

Lab Work

  1. Performed PCR on Dsup, uvsE, phrAC, and phrAT for the GoldenBraid assembly [Collin]
  2. Performed the UV irradiaton test for uvsE, phrAC, and the control [Micah, Mark, Collin]

Outside Work / Discussion

Monday, October 1

No work done today!

Tuesday, October 2

Present: Collin

Lab Work

  1. Measured the ODs of the Cyanobacteria culture [Collin]
    • 3.413 (#1), 0.1370 (#2)

Outside Work / Discussion

Wednesday, October 3

Present:Maddie

Lab Work

  1. Measured the OD of the cyanobacteria culture [Maddie]
    • 0.1452 (#2)

Outside Work / Discussion

Thursday, October 4

Present:Maddie

Lab Work

  1. Measured the OD of the cyanobacteria culture [Maddie]
    • 0.3725 (#2)

Outside Work / Discussion

Friday, October 5

Present:Maddie

Lab Work

  1. Measured the OD of the cyanobacteria culture [Maddie]
    • 0.6746 (#2)

Outside Work / Discussion

Saturday, October 6

Present:Mark

Lab Work

  1. Prepared LB Media [Mark]

Outside Work / Discussion

Sunday, October 7

Present:Mark, Maddie

Lab Work

  1. Prepared LB Agar [Mark]
  2. Prepared overnight cultures of BCP, uvsE, and phrAC [Maddie]

Outside Work / Discussion

Monday, October 8

Present:Mark, Micah

Lab Work

  1. Prepared for a UV irradiation test for uvsE, phrAC, and BCP control [Mark, Micah]

Outside Work / Discussion

Tuesday, October 9

Present: Collin

Lab Work

  1. Measured the OD of the cyanobacteria culture [Maddie]
    • 3.1760 (#2)

Outside Work / Discussion

Wednesday, October 10

Present: Collin

Lab Work

  1. Digested neomycin phosphotransferase (nptII) and pSB1C3 [Colin]
  2. Ran a gel electrophoresis for the digested nptII and pSB1C3 [Collin]
  3. Transformed uvsE+BCP into E. coli [Collin]

Outside Work / Discussion

Thursday, October 11

Present: Collin

Lab Work

  1. Made Kanamycin plates [Collin]
  2. Prepared bacterial stabs of Dsup, phrAC, and phrAT for the UChicago team [Collin]

Outside Work / Discussion

Friday, October 12

No work done today!

Saturday, October 13

No work done today!

Sunday, October 14

No work done today!

Monday, October 15

No work done today!

Tuesday, October 16

Present: Mark

Lab Work

  1. Gel purified pSB1C3 and nptII [Mark]
  2. Ligated pSB1C3 and nptII [Mark]
  3. Transformed pSB1C3+nptII [Mark]

Outside Work / Discussion

Wednesday, October 17

No work done today!

Thursday, October 18

No work done today!

Friday, October 19

Present: Collin

Lab Work

  1. Streaked phrAT, phrAC, BCP, uvsE, and BCP [Collin]

Outside Work / Discussion

Saturday, October 20

Present: Collin

Lab Work

  1. Transformed lac+RBS+Dsup+BCP into E.coli cells [Collin]
  2. Prepared cultures of lac+RBS+Dsup, lac+RBS+BCP, and lac+RBS+uvsE [Collin]

Outside Work / Discussion

Sunday, October 21

Present: Micah

Lab Work

  1. Transformed medium promoter+medium RBS (BBa_K608006) into E. coli [Micah]
  2. Prepared cultures from previous transformation [Micah]

Outside Work / Discussion

Monday, October 22

Present:Collin

Lab Work

  1. Made glycerol stocks of lac+RBS+Dsup+RBS+BCP+pSB1C3 [Collin]

Outside Work / Discussion

Tuesday, October 23

Present: Collin

Lab Work

  1. Miniprepped lac+RBS+BCP+pSB1C3 [Collin]

Outside Work / Discussion

Wednesday, October 24

Giant Jamboree!

Thursday, October 25

Giant Jamboree!

Friday, October 26

Giant Jamboree!

Saturday, October 27

Giant Jamboree!

Sunday, October 28

Giant Jamboree!

Monday, October 29

No work done today!

Tuesday, October 30

No work done today!

Wednesday, October 31

No work done today!