Difference between revisions of "Team:Newcastle/Attributions"
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<font size="5" font face="verdana" font color="green">We will be utilising iGEM registry parts sequenced by IDT to construct a biosynthetic | <font size="5" font face="verdana" font color="green">We will be utilising iGEM registry parts sequenced by IDT to construct a biosynthetic | ||
operon for expression in Escherichia coli. The operon will synthesise naringenin, a flavonoid that attracts nitrogen fixing bacteria. | operon for expression in Escherichia coli. The operon will synthesise naringenin, a flavonoid that attracts nitrogen fixing bacteria. | ||
Revision as of 09:35, 4 September 2018
Alternative Roots
Attributions and
Acknowledgements
2018 Newcastle iGEM
Attributions
Initial Project Idea
- Lewis Tomlinson
- Connor Trotter
- Chris Carty
- Frank Eardley
- Heather Bottomley
- Luke Waller
- Patrycja Ubysz
- Umar Farooq
- Will Tankard
- Sadiya Quazi
Project Support
- Dr. Thomas Howard
- Dr. Jon Marles-Wright
- Dr. Jem Stach
- Dr. Alice Banks
- Dr. Colette Whitfield
- Jasmine Bird
- Alba Iglesias-Vilches
- Dr. Maxim Kapralov
- Dr. Maria Del Carmen Montero-Calasanz
- Dr. Vasilios Andriotis
Chemotaxis
- Connor Trotter
- Sadiya Quazi
- Lewis Tomlinson
- Frank Eardley
- Dr. Alice Banks
Endophyte Development
- Lewis Tomlinson
- Frank Eardley
- Dr. Maria Del Carmen Montero-Calasanz
Operon Development
- Heather Bottomley
- Patrycja Ubysz
Hardware
- Luke Waller
- Umar Farooq
- Chris Carty
- Lewis Tomlinson
Software
- Umar Farooq
- Luke Waller
- Frank Eardley
- Matthew Burridge
Wiki Design
- Umar Farooq
- Will Tankard
- Bradley Brown
Community Modelling
- Patrycja Ubysz
- Dr. Dana Ofiteru
Biosynthesis Pathway Modelling
- Frank Eardley
- Luke Waller
- Umar Farooq
- Josh Isaac
- Aidan Clamp
Human Practices
- Will Tankard
- Chris Carty
- Umar Farooq
- Lewis Tomlinson
- Luke Waller
- Dr. Martyn Dade-Robertson
Outreach and Engagement
- Heather Bottomley
- Will Tankard
Interlab Study
- Matthew Burridge
- Kyle Stanforth
- Sam Went
Acknowledgements
Additional Support
We will be utilising iGEM registry parts sequenced by IDT to construct a biosynthetic operon for expression in Escherichia coli. The operon will synthesise naringenin, a flavonoid that attracts nitrogen fixing bacteria. The operon will contain the genes for four enzymes: 4 – Coumaryl ligase – 4CL (BBa_K1033001), Tyrosine ammonia lyase - TAL (BBa_K1033000), Chalcone isomerase - CHI (BBa_K1497000) and Chalcone synthase - CHS (BBa_K1497001). Each of these parts contain a strong ribosome binding site (BBa_B0034). This construct will be under the control of a T7 promoter (BBa_I712074) to observe its expression in E. coli as a proof of concept. Once biosynthesis under the control of T7 is achieved, the construct will be tested under a constitutive promoter (BBa_J23100). Parts for biosynthesis in the final chassis organism, root-colonising Pseudomonas fluorescens, will be constructed in the plasmid backbone pBSC1C3.