Difference between revisions of "Team:Ecuador/Description"

Line 153: Line 153:
 
   }
 
   }
  
−
   #people {
+
   #parts {
 
     animation-name: slide-in-up-oposite;
 
     animation-name: slide-in-up-oposite;
 
     animation-duration: 0.5s;
 
     animation-duration: 0.5s;
Line 159: Line 159:
 
     animation-fill-mode: backwards;
 
     animation-fill-mode: backwards;
 
     position: absolute;
 
     position: absolute;
−
     left: 14.28%;
+
     left: 12.5%;
 
     z-index: 9;
 
     z-index: 9;
 
   }
 
   }
  
−
   #notebook {
+
   #human_practice {
 
     animation-name: slide-in-up-oposite;
 
     animation-name: slide-in-up-oposite;
 
     animation-duration: 0.5s;
 
     animation-duration: 0.5s;
Line 169: Line 169:
 
     animation-fill-mode: backwards;
 
     animation-fill-mode: backwards;
 
     position: absolute;
 
     position: absolute;
−
     left: 28.56%;
+
     left: 25%;
 
     z-index: 9;
 
     z-index: 9;
 
   }
 
   }
  
−
   #human_practice {
+
   #safety {
 
     animation-name: slide-in-up-oposite;
 
     animation-name: slide-in-up-oposite;
 
     animation-duration: 0.5s;
 
     animation-duration: 0.5s;
Line 179: Line 179:
 
     animation-fill-mode: backwards;
 
     animation-fill-mode: backwards;
 
     position: absolute;
 
     position: absolute;
−
     left: 42.84%;
+
     left: 37.5%;
 
     z-index: 9;
 
     z-index: 9;
 
   }
 
   }
  
−
   #parts {
+
   #interlab {
 
     animation-name: slide-in-up-oposite;
 
     animation-name: slide-in-up-oposite;
 
     animation-duration: 0.5s;
 
     animation-duration: 0.5s;
Line 189: Line 189:
 
     animation-fill-mode: backwards;
 
     animation-fill-mode: backwards;
 
     position: absolute;
 
     position: absolute;
−
     left: 57.12%;
+
     left: 50%;
 
     z-index: 9;
 
     z-index: 9;
 
   }
 
   }
  
−
   #safety {
+
   #judging_forms {
 
     animation-name: slide-in-up-oposite;
 
     animation-name: slide-in-up-oposite;
 
     animation-duration: 0.5s;
 
     animation-duration: 0.5s;
Line 199: Line 199:
 
     animation-fill-mode: backwards;
 
     animation-fill-mode: backwards;
 
     position: absolute;
 
     position: absolute;
−
     left: 71.40%;
+
     left: 62.5%;
 
     z-index: 9;
 
     z-index: 9;
 
   }
 
   }
  
−
   #interlab {
+
   #team {
 
     animation-name: slide-in-up-oposite;
 
     animation-name: slide-in-up-oposite;
 
     animation-duration: 0.5s;
 
     animation-duration: 0.5s;
Line 209: Line 209:
 
     animation-fill-mode: backwards;
 
     animation-fill-mode: backwards;
 
     position: absolute;
 
     position: absolute;
−
     left: 85.68%;
+
     left: 75%;
 +
    z-index: 9;
 +
  }
 +
#atributions {
 +
    animation-name: slide-in-up-oposite;
 +
    animation-duration: 0.5s;
 +
    animation-delay: 1.2s;
 +
    animation-fill-mode: backwards;
 +
    position: absolute;
 +
    left: 87.5%;
 
     z-index: 9;
 
     z-index: 9;
 
   }
 
   }
Line 252: Line 261:
  
 
   .menu_sub_options_container {
 
   .menu_sub_options_container {
−
     width: 90%;
+
     width: 100%;
 
     height: auto;
 
     height: auto;
 
     position: absolute;
 
     position: absolute;
Line 274: Line 283:
  
 
   .sub_menu_option:hover {
 
   .sub_menu_option:hover {
−
     text-indent: 25px;
+
     text-indent: 15px;
 
     background-color: #2E86C1;
 
     background-color: #2E86C1;
 
   }
 
   }
Line 283: Line 292:
 
       position: absolute;
 
       position: absolute;
 
       left: 0px;
 
       left: 0px;
−
       top: 810px;
+
       top: 1380px;
 
       background-color: #212F3C;
 
       background-color: #212F3C;
 
   }
 
   }
Line 413: Line 422:
 
       animation-duration: 4.5s;
 
       animation-duration: 4.5s;
 
     }
 
     }
 +
 +
.ecuador_abstract_image_container {
 +
      width: 55%;
 +
      position: relative;
 +
      height: 100%;
 +
      background-image: url(https://static.igem.org/mediawiki/2018/thumb/c/cb/T--Ecuador--ABSTRACT--001.jpeg/1200px-T--Ecuador--ABSTRACT--001.jpeg);
 +
      background-position: center;
 +
      background-size: 100%;
 +
      background-repeat: no-repeat;
 +
      top: 160px;
 +
      margin: auto;
 +
      animation-name: fade-in;
 +
      animation-duration: 2.5s;
 +
      animation-fill-mode: backwards;
 +
      animation-delay: 1.25s;
 +
    }
 +
  
 
   </style>
 
   </style>
Line 424: Line 450:
 
<div class="menu_options_container">
 
<div class="menu_options_container">
 
  <div id="project" class="menu_option">
 
  <div id="project" class="menu_option">
 +
  <a class="ecuador_href_text" href="https://2018.igem.org/Team:Ecuador/Description">
 
PROJECT
 
PROJECT
 +
  </a>
 
<div class="menu_sub_options_container">
 
<div class="menu_sub_options_container">
 
  <a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Description">
 
  <a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Description">
 
<div class="sub_menu_option">
 
<div class="sub_menu_option">
−
  OVERVIEW
+
  DESCRIPTION
 
</div>
 
</div>
 
  </a>
 
  </a>
Line 446: Line 474:
 
</div>
 
</div>
 
  </a>
 
  </a>
−
</div>
+
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Experiments">
−
  </div>
+
−
  <div id="people" class="menu_option">
+
−
PEOPLE
+
−
<div class="menu_sub_options_container">
+
−
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Sponsiring">
+
−
<div class="sub_menu_option">
+
−
SPONSIRING
+
−
</div>
+
−
</a>
+
−
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Attributions">
+
−
  <div class="sub_menu_option">
+
−
ATTRIBUTIONS
+
−
  </div>
+
−
</a>
+
−
  <a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Team">
+
 
<div class="sub_menu_option">
 
<div class="sub_menu_option">
−
  TEAM
+
  EXPERIMENTS
 
</div>
 
</div>
 
  </a>
 
  </a>
 +
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Notebook">
 +
<div class="sub_menu_option">
 +
  NOTEBOOK
 +
</div>
 +
  </a>
 +
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Model">
 +
<div class="sub_menu_option">
 +
  MODELLING
 +
</div>
 +
  </a>
 +
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Results">
 +
<div class="sub_menu_option">
 +
  RESULTS
 +
</div>
 +
  </a>
 +
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Demonstrate">
 +
<div class="sub_menu_option">
 +
  DEMOSTRATE
 +
</div>
 +
  </a>
 +
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Improve">
 +
<div class="sub_menu_option">
 +
  IMPROVE
 +
</div>
 +
  </a>
 +
 
</div>
 
</div>
 
  </div>
 
  </div>
−
  <div id="notebook" class="menu_option">
+
  <div id="parts" class="menu_option">
−
  <a class="ecuador_href_text" href="https://2018.igem.org/Team:Ecuador/Notebook">
+
  <a class="ecuador_href_text" href="https://2018.igem.org/Team:Ecuador/Parts">
−
NOTEBOOK
+
PARTS
 
  </a>
 
  </a>
 
<div class="menu_sub_options_container">
 
<div class="menu_sub_options_container">
−
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Lab_book">
+
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Parts">
 
  <div class="sub_menu_option">
 
  <div class="sub_menu_option">
−
LAB BOOK
+
  PARTS OVERVIEW
 
  </div>
 
  </div>
 
</a>
 
</a>
−
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Protocols">
+
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Basic">
−
  <div class="sub_menu_option">
+
  <div class="sub_menu_option">
−
  PROTOCOLS
+
  BASIC PARTS
−
  </div>
+
  </div>
 
</a>
 
</a>
 +
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Composite">
 +
  <div class="sub_menu_option">
 +
  COMPOSITE PARTS
 +
  </div>
 +
</a>
 +
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/List">
 +
  <div class="sub_menu_option">
 +
  PARTS COLLECTION
 +
  </div>
 +
</a>
 +
 +
 
</div>
 
</div>
 
  </div>
 
  </div>
−
  <div id="human_practice" class="menu_option">
+
<div id="human_practice" class="menu_option">
 
  <a class="ecuador_href_text" href="https://2018.igem.org/Team:Ecuador/Human_Practices">
 
  <a class="ecuador_href_text" href="https://2018.igem.org/Team:Ecuador/Human_Practices">
−
HUMAN PRACTICE
+
HUMAN PRACTICES
 
  </a>
 
  </a>
 
<div class="menu_sub_options_container">
 
<div class="menu_sub_options_container">
−
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Collaborations">
+
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Human_Practices">
 +
  <div class="sub_menu_option">
 +
HUMAN PRACTICES
 +
  </div>
 +
</a>
 +
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Public_Engagement">
 
  <div class="sub_menu_option">
 
  <div class="sub_menu_option">
−
COLLABORATIONS
+
PUBLIC ENGAGEMENT
 
  </div>
 
  </div>
 
</a>
 
</a>
 
</div>
 
</div>
 
  </div>
 
  </div>
−
  <div id="parts" class="menu_option">
+
<div id="safety" class="menu_option">
−
  <a class="ecuador_href_text" href="https://2018.igem.org/Team:Ecuador/Parts">
+
<a class="ecuador_href_text" href="https://2018.igem.org/Team:Ecuador/Safety">
−
PARTS
+
SAFETY
 +
</a>
 +
  </div>
 +
<div id="interlab" class="menu_option">
 +
<a class="ecuador_href_text" href="http://2018.iGEM.org/Team:Ecuador/Interlab">
 +
  INTERLAB
 +
</a>
 +
  </div>
 +
<div id="judging_forms" class="menu_option">
 +
<a class="ecuador_href_text" href="https://igem.org/2018_Judging_Form?id=2804">
 +
  JUDGING FORMS
 +
</a>
 +
  </div>
 +
 +
  <div id="team" class="menu_option">
 +
  <a class="ecuador_href_text" href="https://2018.igem.org/Team:Ecuador/Team">
 +
TEAM
 
  </a>
 
  </a>
 
<div class="menu_sub_options_container">
 
<div class="menu_sub_options_container">
−
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/List">
+
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Team">
 +
<div class="sub_menu_option">
 +
TEAM MEMBERS
 +
</div>
 +
</a>
 +
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Collaborations">
 
  <div class="sub_menu_option">
 
  <div class="sub_menu_option">
−
  LIST
+
COLLABORATIONS
 
  </div>
 
  </div>
 
</a>
 
</a>
−
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Composite">
+
 
 +
</div>
 +
</div>
 +
 
 +
 +
<div id="atributions" class="menu_option">
 +
<a class="ecuador_href_text" href="https://2018.igem.org/Team:Ecuador/Attributions">
 +
  ATTRIBUTIONS
 +
  </a>
 +
<div class="menu_sub_options_container">
 +
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Attributions">
 
  <div class="sub_menu_option">
 
  <div class="sub_menu_option">
−
  COMPOSITE
+
ATRIBUTTIONS
 
  </div>
 
  </div>
 
</a>
 
</a>
−
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Basic">
+
<a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Sponsoring">
 
  <div class="sub_menu_option">
 
  <div class="sub_menu_option">
−
  BASIC
+
SPONSORING
 
  </div>
 
  </div>
 
</a>
 
</a>
−
</div>
+
</div>
−
  </div>
+
  </div>
−
  <div id="safety" class="menu_option">
+
−
  <a class="ecuador_href_text" href="https://2018.igem.org/Team:Ecuador/Safety">
+
−
SAFETY
+
−
</a>
+
−
  </div>
+
−
+
−
  <div id="interlab" class="menu_option">
+
−
<a class="ecuador_href_text" href="http://2018.iGEM.org/Team:Ecuador/Interlab">
+
−
  INTERLAB
+
−
</a>
+
−
  </div>
+
 
</div>
 
</div>
 
  </div>
 
  </div>
−
 
  
 
   <div class="ecuador_abstract_container">
 
   <div class="ecuador_abstract_container">
Line 541: Line 616:
 
       <div class="ecuador_abstract_text">
 
       <div class="ecuador_abstract_text">
 
         Biomaterials have the ability to interact with biological systems without being rejected. Through interdisciplinary research that involves cell biology, materials science, and bioengineering, biomaterials have been developed as biological substitutes to restore normal cellular function of organs and tissues. A new biomaterial will be developed based on the cross-linking of bacterial cellulose (BC) and elastin-like polypeptides (ELP), for biomedical applications. The cross-linking of both materials will be carried out with synthetic biology techniques using Escherichia coli as expression system. The crosslinking will be mediated by a carbohydrate binding domain protein module (CBD) specific for cellulose. CBDs are protein modules found in carbohydratases whose function is to bind carbohydrate to bring it closer to the catalytic site of the enzyme. For this process, three expression vectors will be designed. The first one will code for the enzymes necessary in the synthesis of bacterial cellulose, the second for the optimization of extracellular production and transport of bacterial cellulose and the third one will code for a fusion protein formed by the elastin-like polypeptide and the carbohydrate binding domain (CBD-ELP). A mathematical model for the interaction between cellulose and the CBD-ELP polypeptide will be determined based on the results from in-vitro experiments. The product generated by the modified bacteria will be characterized by biochemical methods and studied using structural elucidation methods, including spectrophotometry, nuclear magnetic resonance, mass spectrometry, and X-ray diffraction and its morphology will be elucidated using of atomic force microscopy. We expect that the new biomaterial combine the structural properties of bacterial cellulose with the functionalization capacity and thermo-sensitivity of elastin, for applications in food and tissue engineering, regenerative medicine, and selective release of drugs.
 
         Biomaterials have the ability to interact with biological systems without being rejected. Through interdisciplinary research that involves cell biology, materials science, and bioengineering, biomaterials have been developed as biological substitutes to restore normal cellular function of organs and tissues. A new biomaterial will be developed based on the cross-linking of bacterial cellulose (BC) and elastin-like polypeptides (ELP), for biomedical applications. The cross-linking of both materials will be carried out with synthetic biology techniques using Escherichia coli as expression system. The crosslinking will be mediated by a carbohydrate binding domain protein module (CBD) specific for cellulose. CBDs are protein modules found in carbohydratases whose function is to bind carbohydrate to bring it closer to the catalytic site of the enzyme. For this process, three expression vectors will be designed. The first one will code for the enzymes necessary in the synthesis of bacterial cellulose, the second for the optimization of extracellular production and transport of bacterial cellulose and the third one will code for a fusion protein formed by the elastin-like polypeptide and the carbohydrate binding domain (CBD-ELP). A mathematical model for the interaction between cellulose and the CBD-ELP polypeptide will be determined based on the results from in-vitro experiments. The product generated by the modified bacteria will be characterized by biochemical methods and studied using structural elucidation methods, including spectrophotometry, nuclear magnetic resonance, mass spectrometry, and X-ray diffraction and its morphology will be elucidated using of atomic force microscopy. We expect that the new biomaterial combine the structural properties of bacterial cellulose with the functionalization capacity and thermo-sensitivity of elastin, for applications in food and tissue engineering, regenerative medicine, and selective release of drugs.
 +
 
 
       </div>
 
       </div>
 +
 
     </div>
 
     </div>
 +
  <div id="ecuador_abstract_image_container" class="ecuador_abstract_image_container">
 +
</div>
 
   </div>
 
   </div>
  
Line 558: Line 637:
 
           FOLLOW US
 
           FOLLOW US
 
           <div class="ecuador_follow_us_icons_container">
 
           <div class="ecuador_follow_us_icons_container">
 +
  <a href="https://www.facebook.com/BiosinEcuador/">
 
             <div id="facebook" class="ecuador_follow_us_icon">
 
             <div id="facebook" class="ecuador_follow_us_icon">
  
 
             </div>
 
             </div>
 +
  </a>
 +
 
 +
  <a href="https://www.instagram.com/igem.espe.ecuador/">
 
             <div id="instagram" class="ecuador_follow_us_icon">
 
             <div id="instagram" class="ecuador_follow_us_icon">
  
 
             </div>
 
             </div>
 +
  </a>
 
           </div>
 
           </div>
 
         </div>
 
         </div>

Revision as of 20:21, 30 September 2018

iGEM ECUADOR
iGEM ECUADOR
PROJECT OVERVIEW
Biomaterials have the ability to interact with biological systems without being rejected. Through interdisciplinary research that involves cell biology, materials science, and bioengineering, biomaterials have been developed as biological substitutes to restore normal cellular function of organs and tissues. A new biomaterial will be developed based on the cross-linking of bacterial cellulose (BC) and elastin-like polypeptides (ELP), for biomedical applications. The cross-linking of both materials will be carried out with synthetic biology techniques using Escherichia coli as expression system. The crosslinking will be mediated by a carbohydrate binding domain protein module (CBD) specific for cellulose. CBDs are protein modules found in carbohydratases whose function is to bind carbohydrate to bring it closer to the catalytic site of the enzyme. For this process, three expression vectors will be designed. The first one will code for the enzymes necessary in the synthesis of bacterial cellulose, the second for the optimization of extracellular production and transport of bacterial cellulose and the third one will code for a fusion protein formed by the elastin-like polypeptide and the carbohydrate binding domain (CBD-ELP). A mathematical model for the interaction between cellulose and the CBD-ELP polypeptide will be determined based on the results from in-vitro experiments. The product generated by the modified bacteria will be characterized by biochemical methods and studied using structural elucidation methods, including spectrophotometry, nuclear magnetic resonance, mass spectrometry, and X-ray diffraction and its morphology will be elucidated using of atomic force microscopy. We expect that the new biomaterial combine the structural properties of bacterial cellulose with the functionalization capacity and thermo-sensitivity of elastin, for applications in food and tissue engineering, regenerative medicine, and selective release of drugs.
SPONSORS
FOLLOW US
CONTACT US