Difference between revisions of "Team:Tongji China/Description"

This page will give you an overview of our project. The whole process is shown below and following the detailed description.
As is shown above, neoantigens have already shown the promising potential in Individualized cancer treatment (See more details about neoantigens, visit the background-neoantigens). And as we find the efficient protein delivery tool of type III secretion system (T3SS) in P. aeruginosa (See more details about T3SS, visit the background-T3SS), We establish a method which can deliver neoantigens into the immune system using the Type III secretion system of Pseudomonas aeruginosa. We select colorectal cancer as our target and use the bioinformatics method to filter our item antigens. Then we use the T3SS to deliver the item antigens into the immune system through oral intake of engineered attenuated bacteria.

1 We utilize the data resources from TCGA, which contains cancer cells’ SNVs(single nucleotide variations) from many kinds of cancers. We establish a program to filter the antigens we want from these data. What we need to do

2 Antigen sequences which have been filtered will be combined with the Type III secretion system in the attenuated P. aeruginosa. The engineered P. aeruginosa is made into enteric capsule after lyophilization.

3 Through orally intake the enteric capsule, the capsule goes into the intestine and releases the engineered P. aeruginosa. Because the intestine possess its own immune system and there are many interstitial cells including APCs (antigen presenting cells). The engineered P. aeruginosa can attach the APCs which are our target cells.

4 The attachment of the engineered P. aeruginosa and the APCs will trigger the formation of the Type III secretion system injectisome which is colored as red and also trigger the expression of the fusion protein. The fusion protein contains the antigens we filtered above and a T3SS effector or secretion signal and they will go through the injectisome and enter the APCs cytosol. Through this process, the antigens will be successfully injected into the APCs to start the MHC I antigen presenting pathway without being interrupted by the complicated intestine environment.

5 Through the MHC I antigen presenting pathway, the antigens are presented on the APCs membrane for the recognition of the T cells. The T cells recognize and are activated by the antigens through the TCR binds to the presented antigens

6 As the T cells are activated by the antigens, they will have the ability to recognize and kill the tumor cells.

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