1   Dioxin Uptake

Zucchini plants have been shown to have the most successful uptake mechanisms for PCDD/PCDF's of all plants tested. This includes mechanisms that can translocate dioxins from their roots to their shoot system (including flowers and fruits), as opposed to many other plants that can only absorb dioxins into their roots.

Current results demonstrated that cultivation of C. pepo L. cv. ‘Atena Polka’ reduced total PCDD/PCDF content by a mean value of 37 % in soil amended with sewage sludge and 32 % in soil treated with urban sediment (Fig. 1A1, B1; Table 2S). Mean reduction in TEQ concentrations were 68 and 52 % in soil amended with sewage sludge and sediment, respectively; values almost twice those of PCDD/PCDF content (Fig. 1A2, B2; Table 2S). Wilcoxon matched pair test revealed significant differences in total and TEQ values before and after C. pepo L. cv. ‘Atena Polka’ cultivation at p = 0.067. The greatest decline of total PCDD/PCDF content was observed for control samples (66 % for soil with sewage sludge and 81 % for soil with sediment), while the greatest reduction of TEQ values was detected in samples fertilized with 9 and 18 t/ha of sewage sludge (72 and 73 %, respectively) (Fig. 1; Table 2S). In soil amended with 3 t/ha of sludge, ‘Atena Polka’ cultivation led to a 63 % reduction of TEQ. Other large decreases were also noted for soil amended with 9 and 18 t/ha of urban sediments (59 and 70 %, respectively), while a much smaller reduction (21 %) was noted for a dose of 3 t/ha (Fig. 1; Table 2S). The above declines in soil total and TEQ PCDD/PCDF concentrations are, from one site, a result of ‘Atena Polka’ cultivation, however, the bioremediation activity of soil microorganisms seems to also be an important factor responsible for the obtained reductions (Urbaniak 2013; Field and Sierra-Alvarez 2008).

Fig. 1: Mean decreases in total and TEQ PCDD/PCDF concentrations in soil amended with different doses of sewage sludge (A1, A2) and urban sediments (B1–B2) before and after Cucurbita pepo L. cv ‘Atena Polka’ cultivation"

2   Plant Growth

Zucchini requires warm temperatures of above 16 degrees Celsius to grow, but to warm of a climate (above 38 degrees) will harm the plant. The plant takes 60 days to mature, by than it produces fruit as long as someone pick them before they rot. Zucchini has a fast growth and as such, high root uptake of nutrition from the ground. It needs a lot of water to grow, which oppose some problem if you try to grow it in dry areas. The plant itself doesn’t spread much and it`s root go mainly deeper in a taproot formation.

3   Plant Transformation:

Two-week-old in vitro grown Cucurbita pepo L. intact plants and cotyledons (detached and undetached from the mother-plant) were transformed by Agrobacterium rhizogenes strain NCPPB 1855, grown for 48 h at 25 °C on YMB medium. All infected material formed vigorous hairy roots in about seven days. The transformed roots were successfully grown in liquid MS medium without plant growth regulators for an indefinite number of transfers. 

4   Plant Sterility

Triploid plants have larger organs, greater biomass, and strong stress resistance by preserving relatively larger amounts of photosynthetic energy. The undesirable spread of non-native invasive crop and horticultural plants into natural areas can also be reduced or eliminated by the use of triploids, because they tend to be sterile and seedless.

There are few different ways to create triploid plants:

• Natural selection - triploid plants occur in nature, meaning it is possible to look for the desired plant in nature.
• Artificial hybridization - by sexual hybridization of different ploidy, one can create a triploid offspring.
• Endosperm culture in vitro - endosperm is a triploid tissue. Successful regeneration of a plant from an endosperm tissue would result in triploid plant.