Difference between revisions of "Team:HebrewU/Design"

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         These strains were critical for creating useful control groups for our experiment design.
 
         These strains were critical for creating useful control groups for our experiment design.
 
 
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         Our final yeast strain was known in the lab as D24 (Dehalogenase, 2,6,6 hydrolase + 2,3,3 dioxygenase
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        , and 44a dioxygenase) and has a corresponding control strain with 3 vectors allowing for growth on -Leu,
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        Trp, His Dropout media, but containing none of the enzyme from the TCDD degradation pathway.
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Revision as of 17:07, 12 October 2018

HebrewU HujiGEM 2018



Yeast Design




We began creating yeast strains, each with a single vector. After that, we had considered utilizing the mat a/a system for creating strain with more than one vector, but as we found our transformation method extremely efficient, we chose to execute secondary transformations on yeast cells already contain a separate vector. Parallel to these transformations, we engineered control strains that contained the empty vector, with no extra enzymatic activity, but allowed for these stains to be grown on the same selective Drop Out media. These strains were critical for creating useful control groups for our experiment design.




Further Reading: