Difference between revisions of "Team:Newcastle/Results/Chemotaxis/NaringeninMIC"

 
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                 <p class="about-para">Our selected flavonoid, naringenin, is known to possess antimicrobial properties in both gram-positive and gram-negative bacteria [1]. As such, we decided to characterise the effect of naringenin on the growth rate of our bacteria: Azospirillum brasilense, Azorhizobium caulinodans, Herbaspirillum seropedicae, and Escherichia coli. This was done so that we would understand which concentrations of naringenin to use in our chemotaxis assays.</p>
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                 <p class="about-para">In addition to being a chemoattractant, naringenin possess antimicrobial properties against both Gram-positive and Gram-negative bacteria [1]. We therefore characterised the effect that increasing concentrations of naringenin had on the growth rate of <i>Azospirillum brasilense</i>, <i>Azorhizobium caulinodans</i>, <i>Herbaspirillum seropedicae</i>, and <i>E. coli</i>. This information was essential in guiding our chemotaxis assays.</p>
 
              
 
              
 
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                     <p style="font-family:Arial;font-size:100%;color:green;">All species successfully grew in the presence of 0-150μM naringenin (Figure 1). However, it was noted that E. coli showed reduced growth rate even at lower concentration of naringenin. When concentration of naringenin exceeded 100μM, there exists greater flux in all species suggesting that naringenin begins to have a greater impact on some, but not all, bacteria. As such, naringenin concentrations of <100μM were used as part of subsequent chemotaxis assays as to not negatively impact bacterial growth.</p>
 
                     <p style="font-family:Arial;font-size:100%;color:green;">All species successfully grew in the presence of 0-150μM naringenin (Figure 1). However, it was noted that E. coli showed reduced growth rate even at lower concentration of naringenin. When concentration of naringenin exceeded 100μM, there exists greater flux in all species suggesting that naringenin begins to have a greater impact on some, but not all, bacteria. As such, naringenin concentrations of <100μM were used as part of subsequent chemotaxis assays as to not negatively impact bacterial growth.</p>
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<p class="about-para"><font size="3"><b>1. Yang X-e, Wu X, Hao H-l, & He Z-l (2008) Mechanisms and Assessment of Water Eutrophication. Journal of Zhejiang University. Science. B 9(3):197-209.<b></p>
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<p class="about-para"><font size="3"><b>1. 1. Céliz G, Daz M, & Audisio MC (2011) Antibacterial Activity of Naringin Derivatives Against Pathogenic Strains. Journal of Applied Microbiology 111(3):731-738.<b></p>
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<p class="about-para"><font size="3"><b>2. Usman MN, MG; Musa, I (2015) Effect of Three Levels of NPK Fertilizer on Growth Parameters and Yield of Maize-Soybean Intercrop. International Journal of Scientific and Research Publications 5(9).<b></p>
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<p class="about-para"><font size="3"><b>3. Liu W, et al. (2017) Azorhizobium caulinodans Transmembrane Chemoreceptor TlpA1 Involved in Host Colonization and Nodulation on Roots and Stems. Frontiers in Microbiology 8:1327..<b></p>
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<p class="about-para"><font size="3"><b>Attributions:<b></p>
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<p class="about-para"><font size="3"><b>4. Pedrosa FO, et al. (2011) Genome of Herbaspirillum seropedicae Strain SmR1, a Specialized Diazotrophic Endophyte of Tropical Grasses. PLoS Genetics 7(5):e1002064.<b></p>
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<p class="about-para"><font size="3"><b>Primary Author: Connor Trotter<b></p>
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<p class="about-para"><font size="3"><b>Attributions: Connor Trotter<b></p>
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<p class="about-para"><font size="3"><b>Stylisation: Connor Trotter, Umar Farooq<b></p>
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  <p class="about-para"><font size="3"><b>Troubleshooting: Umar Farooq<b></p>
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Latest revision as of 20:32, 13 October 2018

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Alternative Roots

Alternative Roots

Influence of Naringenin on Growth Rate of Bacteria in Liquid Media

Principle Results Graphical Overview
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Principle

In addition to being a chemoattractant, naringenin possess antimicrobial properties against both Gram-positive and Gram-negative bacteria [1]. We therefore characterised the effect that increasing concentrations of naringenin had on the growth rate of Azospirillum brasilense, Azorhizobium caulinodans, Herbaspirillum seropedicae, and E. coli. This information was essential in guiding our chemotaxis assays.

Graphical Overview

INSERT MIC GRAPH HERE

Results

All species successfully grew in the presence of 0-150μM naringenin (Figure 1). However, it was noted that E. coli showed reduced growth rate even at lower concentration of naringenin. When concentration of naringenin exceeded 100μM, there exists greater flux in all species suggesting that naringenin begins to have a greater impact on some, but not all, bacteria. As such, naringenin concentrations of <100μM were used as part of subsequent chemotaxis assays as to not negatively impact bacterial growth.


REFERENCES & Attributions

1. 1. Céliz G, Daz M, & Audisio MC (2011) Antibacterial Activity of Naringin Derivatives Against Pathogenic Strains. Journal of Applied Microbiology 111(3):731-738.

Attributions:

Primary Author: Connor Trotter

Stylisation: Connor Trotter, Umar Farooq

Troubleshooting: Umar Farooq