Difference between revisions of "Team:UChicago/Notebook"

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9/21/18 </br>
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6/14/18</br>
-Prepared PCR of 1.1, 1.3, 2.1, and 3.3</br>
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-Got familiar with lab</br>
&nbsp;&nbsp;&nbsp;&nbsp;-Ran Gel of PCRs</br>
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-Made LB and normal YPAUD liquid media</br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-Well 1 - Ladder</br>
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&nbsp; &nbsp; &nbsp; &nbsp; -Labelled liquid media and left on bench shelf</br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-Well 2 - 1.1</br>
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-Make chloramphenicol plates</br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-Well 3 - 1.3</br>
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&nbsp; &nbsp; &nbsp; &nbsp; -250mg chloramphenicol; marked by CAM</br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-Well 4 - 2.1</br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-Well 5 - 3.3</br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-Imaged Gel</br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-Gel Once again failed, bands did not appear as they were supposed to</br>
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Revision as of 03:09, 14 October 2018

Notebook

6/14/18
-Got familiar with lab
-Made LB and normal YPAUD liquid media
        -Labelled liquid media and left on bench shelf
-Make chloramphenicol plates
        -250mg chloramphenicol; marked by CAM

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