Difference between revisions of "Team:UChicago/Notebook"

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<div class="content">
 
<div class="content">
 
  <p>
 
  <p>
−
6/14/18</br>
+
<b>6/14/18</b></br>
 
-Got familiar with lab</br>
 
-Got familiar with lab</br>
 
-Made LB and normal YPAUD liquid media</br>
 
-Made LB and normal YPAUD liquid media</br>
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&nbsp; &nbsp; &nbsp; &nbsp; -250mg chloramphenicol; marked by CAM</br>
 
&nbsp; &nbsp; &nbsp; &nbsp; -250mg chloramphenicol; marked by CAM</br>
  
 +
</br></br>
 +
 +
<b>6/15/18</b></br></br>
 +
-Made Arg- YPAUD liquid media </br>
 +
&nbsp; &nbsp; &nbsp; &nbsp; (-Used CSM -Arg, -Ura; added uracil in a 1:1 ratio with CSM (1.7g of CSM -Arg -Ura, 1.7g of uracil)</br>
 +
&nbsp; &nbsp; &nbsp; &nbsp; -Marked by 3 blue stripes</br>
 +
-Made Arg- YPAUD plates</br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;-Used CSM -Arg, -Ura; added uracil in a 1:1 ratio with CSM</br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;-Marked by three blue stripes</br>
 +
-PCR amplify arg-ars sequence</br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;-Total volume: 50µL </br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-35µL water</br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-10µL 5X phusion buffer</br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-1µL dNTPs </br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-2.5µL 10µM primer stock (forward and reverse arg-ars primers)</br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-1µL pOW1 (17.6 ng/µL) DNA stock (0.8µL DNA, 4.2µL H2O)</br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-0.5µL phusion</br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-Stored in refrigerator next to PCR machine (labeled iGEM arg-ars PCR)</br>
  
  

Revision as of 03:18, 14 October 2018

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Notebook

6/14/18
-Got familiar with lab
-Made LB and normal YPAUD liquid media
        -Labelled liquid media and left on bench shelf
-Make chloramphenicol plates
        -250mg chloramphenicol; marked by CAM


6/15/18

-Made Arg- YPAUD liquid media
        (-Used CSM -Arg, -Ura; added uracil in a 1:1 ratio with CSM (1.7g of CSM -Arg -Ura, 1.7g of uracil)
        -Marked by 3 blue stripes
-Made Arg- YPAUD plates
    -Used CSM -Arg, -Ura; added uracil in a 1:1 ratio with CSM
    -Marked by three blue stripes
-PCR amplify arg-ars sequence
    -Total volume: 50µL
        -35µL water
        -10µL 5X phusion buffer
        -1µL dNTPs
        -2.5µL 10µM primer stock (forward and reverse arg-ars primers)
        -1µL pOW1 (17.6 ng/µL) DNA stock (0.8µL DNA, 4.2µL H2O)
        -0.5µL phusion
        -Stored in refrigerator next to PCR machine (labeled iGEM arg-ars PCR)

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Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.

Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.