The Project

The overexpression of recombinant proteins in E. coli often result in inclusion bodies. Our project set out to investigate how to prevent them. By using different chaperone systems, which are proteins that assist folding, protection or disaggregation of proteins during stress, a better yield of recombinant proteins can be achieved. Different combinations of these chaperone systems can have different results on folding and aggregation of recombinant proteins. By testing combinations of these systems on hard-to-fold and aggregation prone proteins, a good understanding on how to prevent inclusion bodies can be obtained.