Difference between revisions of "Team:Newcastle/Protocols"
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<td>Endophyte Development</td> | <td>Endophyte Development</td> | ||
<td><a href="https://static.igem.org/mediawiki/2018/6/69/T--Newcastle--Microscopy.docx">View</a></td> | <td><a href="https://static.igem.org/mediawiki/2018/6/69/T--Newcastle--Microscopy.docx">View</a></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Planting Seeds in Agar-filled pipette-tip racks</td> | ||
| + | <td>Lewis Tomlinson</td> | ||
| + | <td>Endophyte Development</td> | ||
| + | <td><a href="https://static.igem.org/mediawiki/2018/9/9e/T--Newcastle--Rack.docx">View</a></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Re-isolating endophytes</td> | ||
| + | <td>Lewis Tomlinson</td> | ||
| + | <td>Endophyte Development</td> | ||
| + | <td><a href="https://static.igem.org/mediawiki/2018/d/d7/T--Newcastle--ReIsolate.docx">View</a></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>Gel extraction and purification of the gblocks</td> |
| − | <td> | + | <td>Heather Bottomley</td> |
| − | <td> | + | <td>Naringenin Operon Biosynthesis</td> |
| − | <td>View</td> | + | <td><a href="https://static.igem.org/mediawiki/2018/d/d1/T--Newcastle--GBlockGelExt.docx">View</a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>Gibson Assembly of gblocks and pSB1C3 backbone</td> |
| − | <td> | + | <td>Heather Bottomley</td> |
| − | <td> | + | <td>Naringenin Operon Biosynthesis</td> |
| − | <td>View</td> | + | <td><a href="https://static.igem.org/mediawiki/2018/0/02/T--Newcastle--Gibson.docx">View</a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>Making chemically competent cells</td> |
| − | <td> | + | <td>Heather Bottomley</td> |
| − | <td> | + | <td>Naringenin Operon Biosynthesis</td> |
| − | <td>View</td> | + | <td><a href="https://static.igem.org/mediawiki/2018/f/ff/T--Newcastle--CompCell.docx">View</a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>Miniprep of transformed colonies</td> |
| − | <td> | + | <td>Heather Bottomley</td> |
| − | <td> | + | <td>Naringenin Operon Biosynthesis</td> |
| − | <td>View</td> | + | <td><a href="https://static.igem.org/mediawiki/2018/b/bf/T--Newcastle--Miniprep.docx">View</a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>Amplification, Purification and Quantification of the pSB1C3 backbone by PCR</td> |
| − | <td> | + | <td>Heather Bottomley</td> |
| − | <td> | + | <td>Naringenin Operon Biosynthesis</td> |
| − | <td>View</td> | + | <td><a href="https://static.igem.org/mediawiki/2018/c/c4/T--Newcastle--PCR.docx">View</a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>Quantifying DNA concentration</td> |
| − | <td> | + | <td>Heather Bottomley</td> |
| − | <td> | + | <td>Naringenin Operon Biosynthesis</td> |
| − | <td>View</td> | + | <td><a href="https://static.igem.org/mediawiki/2018/4/4f/T--Newcastle--QuantDNA.docx">View</a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>Resuspending gblocks and primers</td> |
| − | <td> | + | <td>Heather Bottomley</td> |
| − | <td> | + | <td>Naringenin Operon Biosynthesis</td> |
| − | <td>View</td> | + | <td><a href="https://static.igem.org/mediawiki/2018/3/32/T--Newcastle--GBlockFragments.docx">View</a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>SOC media preparation</td> |
| − | <td> | + | <td>Heather Bottomley</td> |
| − | <td> | + | <td>Naringenin Operon Biosynthesis</td> |
| − | <td>View</td> | + | <td><a href="https://static.igem.org/mediawiki/2018/9/99/T--Newcastle--SOC.docx">View</a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>Transformation of the gblocks into pSB1C3</td> |
| − | <td> | + | <td>Heather Bottomley</td> |
| − | <td> | + | <td>Naringenin Operon Biosynthesis</td> |
| − | <td> | + | <td><a href="https://static.igem.org/mediawiki/2018/2/2d/T--Newcastle--GBlockTransform.docx">View</a></td> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</tr> | </tr> | ||
</tbody> | </tbody> | ||
Revision as of 20:57, 14 October 2018
Experiments
Protocols
EXPERIMENTS
PROTOCOLS
| Protocol | Author | Category | PDF File |
|---|---|---|---|
| Glycerol Stock Preserves | Connor Trotter | General | View |
| Bacterial Isolation from Freeze-Dried Pellet | Connor Trotter | General | View |
| Cell Counting | Connor Trotter | Chemotaxis | View |
| Minimal A Salt Agar Chemotaxis Assay | Connor Trotter | Chemotaxis | View |
| Planting Seeds in Agar-filled Microcentrifuge tubes | Lewis Tomlinson | Endophyte Development | View |
| Preparation and Staining of Microscopy samples | Lewis Tomlinson | Endophyte Development | View |
| Planting Seeds in Agar-filled pipette-tip racks | Lewis Tomlinson | Endophyte Development | View |
| Re-isolating endophytes | Lewis Tomlinson | Endophyte Development | View |
| Amplification of gblocks and gradient PCR | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Colony PCR of transformed DH5α cells | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| DPN1 digestion and purification | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Gel extraction and purification of the gblocks | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Gibson Assembly of gblocks and pSB1C3 backbone | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Making chemically competent cells | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Miniprep of transformed colonies | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Amplification, Purification and Quantification of the pSB1C3 backbone by PCR | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Quantifying DNA concentration | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Resuspending gblocks and primers | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| SOC media preparation | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Transformation of the gblocks into pSB1C3 | Heather Bottomley | Naringenin Operon Biosynthesis | View |