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Revision as of 21:56, 14 October 2018

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Experiments

Protocols

Overview

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EXPERIMENTS

PROTOCOLS

Protocol	Author	Category	PDF File
Glycerol Stock Preserves	Connor Trotter	General	View
Bacterial Isolation from Freeze-Dried Pellet	Connor Trotter	General	View
Cell Counting	Connor Trotter	Chemotaxis	View
Minimal A Salt Agar Chemotaxis Assay	Connor Trotter	Chemotaxis	View
Planting Seeds in Agar-filled Microcentrifuge tubes	Lewis Tomlinson	Endophyte Development	View
Preparation and Staining of Microscopy samples	Lewis Tomlinson	Endophyte Development	View
Planting Seeds in Agar-filled pipette-tip racks	Lewis Tomlinson	Endophyte Development	View
Re-isolating endophytes	Lewis Tomlinson	Endophyte Development	View
Electroporation of Pseudomonas sp.	Frank Eardley	Endophyte Development	View
MIC Opentrons Code (2 Plates)	Frank Eardley	Endophyte Development	View
Amplification of gblocks and gradient PCR	Heather Bottomley	Naringenin Operon Biosynthesis	View
Colony PCR of transformed DH5α cells	Heather Bottomley	Naringenin Operon Biosynthesis	View
DPN1 digestion and purification	Heather Bottomley	Naringenin Operon Biosynthesis	View
Gel extraction and purification of the gblocks	Heather Bottomley	Naringenin Operon Biosynthesis	View
Gibson Assembly of gblocks and pSB1C3 backbone	Heather Bottomley	Naringenin Operon Biosynthesis	View
Making chemically competent cells	Heather Bottomley	Naringenin Operon Biosynthesis	View
Miniprep of transformed colonies	Heather Bottomley	Naringenin Operon Biosynthesis	View
Amplification, Purification and Quantification of the pSB1C3 backbone by PCR	Heather Bottomley	Naringenin Operon Biosynthesis	View
Quantifying DNA concentration	Heather Bottomley	Naringenin Operon Biosynthesis	View
Resuspending gblocks and primers	Heather Bottomley	Naringenin Operon Biosynthesis	View
SOC media preparation	Heather Bottomley	Naringenin Operon Biosynthesis	View
Transformation of the gblocks into pSB1C3	Heather Bottomley	Naringenin Operon Biosynthesis	View