Difference between revisions of "Team:Tongji China/Protocol"
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<summary>Chemical Transformation</summary> | <summary>Chemical Transformation</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/b/b7/T--Tongji_China--protocol-1-2.pdf" internalinstanceid="10"> | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/b/b7/T--Tongji_China--protocol-1-2.pdf" internalinstanceid="10"><br> |
<a href="https://static.igem.org/mediawiki/2018/b/b7/T--Tongji_China--protocol-1-2.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/b/b7/T--Tongji_China--protocol-1-2.pdf" download="">Download</a> | ||
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<summary>Colnony PCR</summary> | <summary>Colnony PCR</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/b/bc/T--Tongji_China--protocol-1-3.pdf" width="800" internalinstanceid="14"> | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/b/bc/T--Tongji_China--protocol-1-3.pdf" width="800" internalinstanceid="14"><br> |
<a href="https://static.igem.org/mediawiki/2018/b/bc/T--Tongji_China--protocol-1-3.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/b/bc/T--Tongji_China--protocol-1-3.pdf" download="">Download</a> | ||
</details> | </details> | ||
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<summary>Consumables</summary> | <summary>Consumables</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/2/25/T--Tongji_China--protocol-1-4.pdf" width="800" internalinstanceid="14"> | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/2/25/T--Tongji_China--protocol-1-4.pdf" width="800" internalinstanceid="14"><br> |
<a href="https://static.igem.org/mediawiki/2018/2/25/T--Tongji_China--protocol-1-4.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/2/25/T--Tongji_China--protocol-1-4.pdf" download="">Download</a> | ||
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<summary>DNA Gel Electrophoresis</summary> | <summary>DNA Gel Electrophoresis</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/1/10/T--Tongji_China--protocol-1-5.pdf" width="800"> | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/1/10/T--Tongji_China--protocol-1-5.pdf" width="800"><br> |
<a href="https://static.igem.org/mediawiki/2018/1/10/T--Tongji_China--protocol-1-5.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/1/10/T--Tongji_China--protocol-1-5.pdf" download="">Download</a> | ||
</details> | </details> | ||
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<summary>Gel Extraction & Minipreps</summary> | <summary>Gel Extraction & Minipreps</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/3/37/T--Tongji_China--protocol-1-6.pdf" width="800"> | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/3/37/T--Tongji_China--protocol-1-6.pdf" width="800"><br> |
<a href="https://static.igem.org/mediawiki/2018/3/37/T--Tongji_China--protocol-1-6.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/3/37/T--Tongji_China--protocol-1-6.pdf" download="">Download</a> | ||
</details> | </details> | ||
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<summary>Ligation</summary> | <summary>Ligation</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/e/e3/T--Tongji_China--protocol-1-7.pdf" width="800"> | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/e/e3/T--Tongji_China--protocol-1-7.pdf" width="800"><br> |
<a href="https://static.igem.org/mediawiki/2018/e/e3/T--Tongji_China--protocol-1-7.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/e/e3/T--Tongji_China--protocol-1-7.pdf" download="">Download</a> | ||
</details> | </details> | ||
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<summary>Restriction Digestion</summary> | <summary>Restriction Digestion</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/2/2d/T--Tongji_China--protocol-1-8.pdf" width="800"> | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/2/2d/T--Tongji_China--protocol-1-8.pdf" width="800"><br> |
<a href="https://static.igem.org/mediawiki/2018/2/2d/T--Tongji_China--protocol-1-8.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/2/2d/T--Tongji_China--protocol-1-8.pdf" download="">Download</a> | ||
</details> | </details> | ||
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<summary>Colony PCR of Pseudomonas aeruginosa</summary> | <summary>Colony PCR of Pseudomonas aeruginosa</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/5/50/T--Tongji_China--protocol-2-1.pdf" width="800"> | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/5/50/T--Tongji_China--protocol-2-1.pdf" width="800"><br> |
<a href="https://static.igem.org/mediawiki/2018/5/50/T--Tongji_China--protocol-2-1.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/5/50/T--Tongji_China--protocol-2-1.pdf" download="">Download</a> | ||
</details> | </details> | ||
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<summary>Creating Bacterial Glycerol Stocks</summary> | <summary>Creating Bacterial Glycerol Stocks</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-2-2.pdf" width="800"> | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-2-2.pdf" width="800"><br> |
<a href="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-2-2.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-2-2.pdf" download="">Download</a> | ||
</details> | </details> | ||
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<summary>Electroporation</summary> | <summary>Electroporation</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/2/2f/T--Tongji_China--protocol-2-3.pdf" width="800"> | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/2/2f/T--Tongji_China--protocol-2-3.pdf" width="800"><br> |
<a href="https://static.igem.org/mediawiki/2018/2/2f/T--Tongji_China--protocol-2-3.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/2/2f/T--Tongji_China--protocol-2-3.pdf" download="">Download</a> | ||
</details> | </details> | ||
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<summary>Western Blot</summary> | <summary>Western Blot</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/8/8e/T--Tongji_China--protocol-2-4.pdf" width="800"> | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/8/8e/T--Tongji_China--protocol-2-4.pdf" width="800"><br> |
<a href="https://static.igem.org/mediawiki/2018/8/8e/T--Tongji_China--protocol-2-4.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/8/8e/T--Tongji_China--protocol-2-4.pdf" download="">Download</a> | ||
</details> | </details> | ||
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<summary>Immunofluorescence Staining</summary> | <summary>Immunofluorescence Staining</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/6/62/T--Tongji_China--protocol-3-1.pdf" width="800"> | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/6/62/T--Tongji_China--protocol-3-1.pdf" width="800"><br> |
<a href="https://static.igem.org/mediawiki/2018/6/62/T--Tongji_China--protocol-3-1.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/6/62/T--Tongji_China--protocol-3-1.pdf" download="">Download</a> | ||
</details> | </details> | ||
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<summary>Passage HeLa Cells</summary> | <summary>Passage HeLa Cells</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-3-2.pdf" width="800"> | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-3-2.pdf" width="800"><br> |
<a href="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-3-2.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-3-2.pdf" download="">Download</a> | ||
</details> | </details> | ||
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<summary>Thawing Frozen Cells</summary> | <summary>Thawing Frozen Cells</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/f/fe/T--Tongji_China--protocol-3-3.pdf" width="800"> | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/f/fe/T--Tongji_China--protocol-3-3.pdf" width="800"><br> |
<a href="https://static.igem.org/mediawiki/2018/f/fe/T--Tongji_China--protocol-3-3.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/f/fe/T--Tongji_China--protocol-3-3.pdf" download="">Download</a> | ||
</details> | </details> | ||
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<summary>Oral Gavage of Mice</summary> | <summary>Oral Gavage of Mice</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/b/b4/T--Tongji_China--protocol-4-1.pdf" width="800"> | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/b/b4/T--Tongji_China--protocol-4-1.pdf" width="800"><br> |
<a href="https://static.igem.org/mediawiki/2018/b/b4/T--Tongji_China--protocol-4-1.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/b/b4/T--Tongji_China--protocol-4-1.pdf" download="">Download</a> | ||
</details> | </details> | ||
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<summary>Immunohistochemistry</summary> | <summary>Immunohistochemistry</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/4/43/T--Tongji_China--protocol-4-2.pdf" width="800"> | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/4/43/T--Tongji_China--protocol-4-2.pdf" width="800"><br> |
<a href="https://static.igem.org/mediawiki/2018/4/43/T--Tongji_China--protocol-4-2.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/4/43/T--Tongji_China--protocol-4-2.pdf" download="">Download</a> | ||
</details> | </details> | ||
Revision as of 02:06, 16 October 2018
Wet Lab
Protocol
.
Plasmid
Annealing Oilgos
Chemical Transformation
Colnony PCR
Consumables
DNA Gel Electrophoresis
Gel Extraction & Minipreps
Ligation
Restriction Digestion
.
Pseudomonas aeruginosa
Colony PCR of Pseudomonas aeruginosa
Creating Bacterial Glycerol Stocks
Electroporation
Western Blot
.
Hela Cell
Immunofluorescence Staining
Passage HeLa Cells
Thawing Frozen Cells
.