Difference between revisions of "Team:Tufts/Notebook"

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<h1>Notebook</h1>
 
<h1>Notebook</h1>
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<p> Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.</p>
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<p> <b>Project Timeline</b></p>
  
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<p>5/27-6/2: Ordered all necessary DNA materials </p>
  
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<p>6/3-6/9: Worked on acquiring lab access for all members that would be researching in the summer </p>
  
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<p>6/10-6/16: Developed protocols for how to execute the design of the project</p>
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<h3>What should this page have?</h3>
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<ul>
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<li>Chronological notes of what your team is doing.</li>
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<li> Brief descriptions of daily important events.</li>
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<li>Pictures of your progress. </li>
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<li>Mention who participated in what task.</li>
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<p>6/17-6/23: Moved into new facilities, Tufts Science & Engineering Center</p>
  
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<p>6/24-6/30: Prepped DNA and lab materials, including diluting the DNA</p>
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<h3>Inspiration</h3>
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<p>You can see what others teams have done to organize their notes:</p>
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<p>7/1-7/7: Attempted to assembly PCR the two overlapping gene blocks of Cas13a unsuccessfully</p>
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<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
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<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
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<p>7/8-7/14: Collaborated with Josef Bober from the Nair lab in order to establish a better PCR protocol</p>
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<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
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<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
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<p>7/15-7/21: After several failed assembly attempts, new primers were ordered in case the ones previously used had degraded</p>
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<p>7/22-7/28: Pause in work before the new primers arrived. Ordered geneblocks and emailed iGEM organization about necessary missing DNA kit and current transformed bacteria. Extension for interlab project given. </p>
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<p>7/29-8/4: Interlab procedure preparation was completed.</p>
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<p>8/5-8/11: Interlab days 1-3 were completed. </p>
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<p>8/12-8/18: Interlab cell counting was completed. Equipment was inventoried with refrigerator space shared with graduate student lab in facilities. </p>
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<p>8/19-8/25: The interlab data was submitted. Assembly PCR with new primers was performed. This were somewhat effective, but produced a smear, leading us to conclude that the DNA was degraded. At this point, the primary project was abandoned, as there was not sufficient time to order new DNA and PCR the toehold. </p>
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<p>8/26-9/1: Break prior to the start of the semester </p>
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<p>9/2-9/8: Break during start of the semester - Tufts University classes begin</p>
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<p>9/9-9/15: iGEM website </p>
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<p>9/16-9/22: iGEM outreach with Student Campus Activity Fair. General interest meeting and presentations given on iGEM summer research and competition. </p>
 
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Latest revision as of 03:04, 16 October 2018

HOME
TEAM MEMBERS
PROJECT
Description
Design
Experiments
Notebook
InterLab
Results
Attributions
SAFETY
HUMAN PRACTICES
Human Practices
Education & Engagement
AWARDS
JUDGING FORM ⇗

Notebook

Project Timeline

5/27-6/2: Ordered all necessary DNA materials

6/3-6/9: Worked on acquiring lab access for all members that would be researching in the summer

6/10-6/16: Developed protocols for how to execute the design of the project

6/17-6/23: Moved into new facilities, Tufts Science & Engineering Center

6/24-6/30: Prepped DNA and lab materials, including diluting the DNA

7/1-7/7: Attempted to assembly PCR the two overlapping gene blocks of Cas13a unsuccessfully

7/8-7/14: Collaborated with Josef Bober from the Nair lab in order to establish a better PCR protocol

7/15-7/21: After several failed assembly attempts, new primers were ordered in case the ones previously used had degraded

7/22-7/28: Pause in work before the new primers arrived. Ordered geneblocks and emailed iGEM organization about necessary missing DNA kit and current transformed bacteria. Extension for interlab project given.

7/29-8/4: Interlab procedure preparation was completed.

8/5-8/11: Interlab days 1-3 were completed.

8/12-8/18: Interlab cell counting was completed. Equipment was inventoried with refrigerator space shared with graduate student lab in facilities.

8/19-8/25: The interlab data was submitted. Assembly PCR with new primers was performed. This were somewhat effective, but produced a smear, leading us to conclude that the DNA was degraded. At this point, the primary project was abandoned, as there was not sufficient time to order new DNA and PCR the toehold.

8/26-9/1: Break prior to the start of the semester

9/2-9/8: Break during start of the semester - Tufts University classes begin

9/9-9/15: iGEM website

9/16-9/22: iGEM outreach with Student Campus Activity Fair. General interest meeting and presentations given on iGEM summer research and competition.