Difference between revisions of "Team:BIT-China/Safety"

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{{BIT-China}}
 
 
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<h1> Safety </h1>
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<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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</head>
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<h3>Safe Project Design</h3>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<body id="ibody" class="scoll_dis">
  
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<ul>
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    <ul id="left-nav">
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<li>Choosing a non-pathogenic chassis</li>
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        <li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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            <a>PROJECT</a>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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            <ul>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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                <li><a href="https://2018.igem.org/Team:BIT-China/Background">Background</a></li>
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</ul>
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                <li><a href="https://2018.igem.org/Team:BIT-China/Description">Description</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Design">Idea & Design</a></li>
 +
            </ul>
 +
        </li>
  
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</div>
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        <li>
 +
            <a>EXPERIMENTS</a>
 +
            <ul>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsRegulator">Regulator</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsFeedback">Feedback</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsOutput">Output</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsInput">Input</a></li>
 +
            </ul>
 +
        </li>
  
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<div class="column third_size">
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        <li>
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<h3>Safe Lab Work</h3>
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            <a>MODELING</a>
 +
            <ul>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Model">Overview</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/FluorescentProbesModel">Fluorescent Probes Model </a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/H2O2DecompositionModel">H<sub>2</sub>O<sub>2</sub>
 +
                        Decomposition Model</a></li>
  
−
<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
+
                <li><a href="https://2018.igem.org/Team:BIT-China/roGFP2-Orp1MichaelisEquationModel">roGFP2-Orp1
 +
                        Michaelis equations Model</a></li>
 +
            </ul>
 +
        </li>
  
 +
        <li>
 +
            <a>HUMAN PRACTICES</a>
 +
            <ul>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/HPOverview">Overview</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Human_Practices">Integrated Human Practices</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Public_Engagement">Education & Public Engagement</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Collaborations">Collaborations</a></li>
 +
            </ul>
 +
        </li>
  
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<h3>Safe Shipment</h3>
+
        <li>
 +
            <a>NOTEBOOK</a>
 +
            <ul>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Notebook">Lab Book</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Protocols">Methodology / Protocols</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Equipment">Material & Equipment</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/InterLab">Measurement / InterLab</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Safety">Safety</a></li>
 +
            </ul>
 +
        </li>
  
−
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
+
        <li>
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</div>
+
            <a>ACHIEVEMENTS</a>
 +
            <ul>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/JudgingForm">Judging Form</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Parts">Parts</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Improve">Improve</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Applied_Design">Applied Design</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Demonstrate">Demonstrate</a></li>
 +
            </ul>
 +
        </li>
  
 +
        <li>
 +
            <a>TEAM</a>
 +
            <ul>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Team">Members</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Attributions">Attributions</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Gallery">Gallery</a></li>
 +
            </ul>
 +
        </li>
 +
    </ul>
 +
 +
    <a href="https://2018.igem.org/Team:BIT-China"><img id="imgA" class="imgA-new-pos" src="https://static.igem.org/mediawiki/2018/4/46/T--BIT-China--iGEM2018-A_img.png" /></a>
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            <div class="NOT-title">
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                <a class="NOT-title-1" style="z-index:1;border-bottom: 3px solid #131313;">Safety</a>
 +
            </div>
 +
 +
            <div class="NOT-overview NOT-margin-toTitle">
 +
                <p class="NOT-content-p">
 +
                    Every member of our team has been trained about general lab safety and project design safety by our
 +
                    previous members. For example, the former leader and our advisors taught us how to run the
 +
                    experiment correctly and avoid mistakes.
 +
                </p>
 +
            </div>
 +
        </div>
 +
 +
        <div id="NOT1" class="cd-section">
 +
            <div class="NOT-title-2 NOT-margin-title2ToUp">
 +
                <a>PROJECT DESIGN SAFETY</a>
 +
            </div>
 +
 +
            <div class="NOT-content-all">
 +
                <div class="NOT-content">
 +
                    <p class="NOT-content-p NOT-margin-toTitle2">
 +
                        For the safety of our team members, we designed the project to be as safe as possible. The core
 +
                        of our project is to accumulate intracellular ROS by overexpressing the endogenous ndi1 gene of
 +
                        Saccharomyces cerevisiae. And in order to improve yeast survival, we use a feedback system to
 +
                        maintain intracellular ROS at an appropriate level. The entire project is harmless to the human
 +
                        body. During the implementation of the project, we selected the roGFP2-Orp1 fusion protein as a
 +
                        reporter gene, which can be directly detected without adding any harmful reagents.
 +
                    </p>
 +
                </div>
 +
            </div>
 +
 +
        </div>
 +
 +
        <div id="NOT2" class="cd-section">
 +
            <div class="NOT-title-2 NOT-margin-title2ToUp">
 +
                <a>LAB SAFETY</a>
 +
            </div>
 +
 +
            <div class="NOT-content-all">
 +
                <div class="NOT-content">
 +
                    <p class="NOT-content-p NOT-margin-toTitle2">
 +
                        Our team conducted research in a safety level 1/2 laboratory. The organisms we used, E. coli
 +
                        strain DH5α, BL21, Top 10 and S.c strain CEN.PK2-1C are suitable for the S1/2-safety laboratory
 +
                        we are working in. Besides, the plasmids we are using for transformation (pRS42K, pESC-Ura,
 +
                        pESC-Trp, pESC-His, pESC-Leu, pSB1C3) are not harmful for humans.
 +
                    </p>
 +
 +
                    <p class="NOT-content-p NOT-margin-toP">
 +
                        See more in <a href="https://2018.igem.org/Team:BIT-China/Equipment" style="color:#131313;">Notebook
 +
                            /Equipment &
 +
                            Material</a>.
 +
                    </p>
 +
 +
                    <p class="NOT-content-p NOT-margin-toP">
 +
                        Because of the nature of our project, contacting with microorganisms is inevitable. In order to
 +
                        avoid any harmful effects to each member of our team, all laboratory work was in accordance
 +
                        with the safety requirements for the laboratory. This includes, but is not limited to, the
 +
                        following examples:
 +
                    </p>
 +
 +
                    <ul class="NOT-content-p NOT-margin-toP">
 +
                        <li>(1) Eating, drinking and smoking are prohibited in the laboratory.</li>
 +
                        <li>(2) Don’t deal with the wastes at will.</li>
 +
                        <li>(3) All experimenters must wear protective equipment such as gloves and Lab coats.</li>
 +
                        <li>(4) All equipments that come into contact with biological material (such as tips and glass
 +
                            glassware) must be autoclaved.</li>
 +
                        <li>(5) Store quick-burning, flammable materials in designated locations which are away from
 +
                            ignition sources for fire protection. And there is no accumulated clutter in the hallway,
 +
                            for our safety while accident happening.</li>
 +
                        <li>
 +
                            Laboratory for experiments, seminar rooms for research and discussion, and the office used
 +
                            to search for information and analyze data are rigorously divided. These provisions are
 +
                            intended to prevent the possibility of unnecessary cross-contamination in the laboratory.
 +
                        </li>
 +
                    </ul>
 +
                </div>
 +
            </div>
 +
        </div>
 +
 +
        <div id="NOT3" class="cd-section">
 +
            <div class="NOT-title-2 NOT-margin-title2ToUp">
 +
                <a>DANGEROUS CHEMICALS</a>
 +
            </div>
 +
 +
            <div class="NOT-content-all">
 +
                <div class="NOT-content">
 +
                    <div class="NOT-content-p NOT-margin-toTitle2">
 +
                        We tried to avoid the use of dangerous chemicals whenever possible. We have completely banned a
 +
                        number of dangerous chemicals, such as ethidium bromide which had been replaced by anthocyanin
 +
                        for our DNA gel electrophoresis. At the same time, we have restricted the use of dangerous
 +
                        chemicals (such as acrylamide) which must be clearly placed in marked areas in the laboratory.
 +
                        And we were also required to keep the chemistry reagents in classified storage.
 +
                    </div>
 +
                </div>
 +
            </div>
 +
        </div>
 +
    </div>
 +
 +
    <!-- footer start -->
 +
    <div class="footer-all" style="margin-top:65px;">
 +
        <div class="footer-left">
 +
            <img src="https://static.igem.org/mediawiki/2018/c/ca/T--BIT-China--iGEM2018-sponsor-1.png">
 +
            <img src="https://static.igem.org/mediawiki/2018/0/07/T--BIT-China--iGEM2018-sponsor-2.png">
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            <p style="font-family:'kg_second_chances_solidRg';font-size:12px;margin: 0;padding: 0 0 3px;margin-top: 8px;">Contact</p>
 +
            <p style="font-size:15px;line-height: 1.5 !important;margin: 0;padding: 0;margin-top: 6px;">Institute of
 +
                Biotransformation and synthetic biosystem School of Chemistry and Chemical Engineering.
 +
            </p>
 +
            <p style="font-size:15px;line-height: 1.5 !important;margin: 0;padding: 0;margin-top: 6px;">
 +
                Beijing Institute of Technology
 +
            </p>
 +
            <p style="font-size:15px;line-height: 1.5 !important;margin: 0;padding: 0;margin-top: 6px;">
 +
                100081, Beijing
 +
            </p>
 +
            <p style="font-size:15px;line-height: 1.5 !important;margin: 0;padding: 0;margin-top: 6px;">
 +
                Email:lichun@bit.edu.cn
 +
            </p>
 +
 +
            <p style="font-size:15px;margin: 0;padding: 0;margin-top: 6px;color:  rgb(161, 161, 161);">
 +
                Copyright © 2018 BIT-China
 +
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Revision as of 13:29, 16 October 2018

Safety

Every member of our team has been trained about general lab safety and project design safety by our previous members. For example, the former leader and our advisors taught us how to run the experiment correctly and avoid mistakes.

PROJECT DESIGN SAFETY

For the safety of our team members, we designed the project to be as safe as possible. The core of our project is to accumulate intracellular ROS by overexpressing the endogenous ndi1 gene of Saccharomyces cerevisiae. And in order to improve yeast survival, we use a feedback system to maintain intracellular ROS at an appropriate level. The entire project is harmless to the human body. During the implementation of the project, we selected the roGFP2-Orp1 fusion protein as a reporter gene, which can be directly detected without adding any harmful reagents.

LAB SAFETY

Our team conducted research in a safety level 1/2 laboratory. The organisms we used, E. coli strain DH5α, BL21, Top 10 and S.c strain CEN.PK2-1C are suitable for the S1/2-safety laboratory we are working in. Besides, the plasmids we are using for transformation (pRS42K, pESC-Ura, pESC-Trp, pESC-His, pESC-Leu, pSB1C3) are not harmful for humans.

See more in Notebook /Equipment & Material.

Because of the nature of our project, contacting with microorganisms is inevitable. In order to avoid any harmful effects to each member of our team, all laboratory work was in accordance with the safety requirements for the laboratory. This includes, but is not limited to, the following examples:

  • (1) Eating, drinking and smoking are prohibited in the laboratory.
  • (2) Don’t deal with the wastes at will.
  • (3) All experimenters must wear protective equipment such as gloves and Lab coats.
  • (4) All equipments that come into contact with biological material (such as tips and glass glassware) must be autoclaved.
  • (5) Store quick-burning, flammable materials in designated locations which are away from ignition sources for fire protection. And there is no accumulated clutter in the hallway, for our safety while accident happening.
  • Laboratory for experiments, seminar rooms for research and discussion, and the office used to search for information and analyze data are rigorously divided. These provisions are intended to prevent the possibility of unnecessary cross-contamination in the laboratory.
DANGEROUS CHEMICALS
We tried to avoid the use of dangerous chemicals whenever possible. We have completely banned a number of dangerous chemicals, such as ethidium bromide which had been replaced by anthocyanin for our DNA gel electrophoresis. At the same time, we have restricted the use of dangerous chemicals (such as acrylamide) which must be clearly placed in marked areas in the laboratory. And we were also required to keep the chemistry reagents in classified storage.

Contact

Institute of Biotransformation and synthetic biosystem School of Chemistry and Chemical Engineering.

Beijing Institute of Technology

100081, Beijing

Email:lichun@bit.edu.cn

Copyright © 2018 BIT-China