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Revision as of 13:34, 16 October 2018
- Addon: ENABLEribo
- Addon: ENABLETALE
- Addon: ENABLET2
- Model: transcriptional amplifer
- Model: war predictor
- Software
Collaborations
Working together, Team:Fudan-CHINA
Assembled around the same time and representing the same university, we have since built a strong and cooperative relationship. We Team:Fudan and their Team:Fudan-CHINA, have always been on a fantastic and harmonious journey together, aiding each other and providing useful feedbacks whenever we meet and discuss. We frequently share our plasmids and reagents to them, and also provide them with immediate help on experiments including cell culture, fluorescent imaging, and flow cytometry. Vice versa, they assisted us with the interlab, specially the plate reader.
With common interests, Team:NAU-CHINA
As Team:NAU-CHINA in Nanjing found out that we had parts [BB111111] [BB22222] that they could use to build their genetic circuits, they reached out to us, and we gladly provided them with these plasmids with detailed sequence information and instructions on how to use. The parts we provided were ultimately very effective in constructing and testing their signal transduction system. The parts and the plasmids that contain them are listed below: * SynNotch【提供给南京农业大学的SynNotch质粒图】旧版本是不是要说明 * surEGFP (surface EGFP)【质粒图】是不是要有之前实验数据图/链接回
☺ Manufacturing and distributing, Team:OUC-China
Sharing a similar idea on how to promote basic sciences, Team:OUC-China and we Team:Fudan both wanted to provide a visual and entertaining way for people to understand synthetic biology. With this idea in mind, they created a comic book on theories and technologies in Synthetic Biology. We helped promote synthetic biology by distributing and displaying many of their book in our Bio-Art display and school fair to grab the interest of the audience.
Jumpstart, high school Team:Nanjing_NFLS
As it is the first time for them to participate in iGEM, we provided them with many advice including wiki construction and scientific writing. With our help, they have obtained sufficient knowledge in scientific presentation, and scientific communication. They were able to express their preliminary ideas and results fluently in English and were more confident in presenting their project.
Our cornerstone, Team:Tsinghua
With a long-standing friendship with Team:Tsinghua, we had extensive discussions over Internet and when we met inCCiC, where we exchanged a myriad of ideas covering project design, human practice, team management and so on. By the way, they gave us valuable suggestions on our poster design
Heading south, the iGEM EuroAsian Meetup
We participated in the iGEM EuroAsian Meetup which was from July 24th to 26th, hosted by TUDelft and BGIC-Global in Shenzhen. This July trip was our first opportunity to share our project internationally. The Meetup has provided us with many valuable suggestions. For example, since then we started to focus more intensely on how we could help our general audience better understand our project. It was primarily thanks to the workshop sessions where we had to come up with solutions to promote our products or human practice within \[just 1 minute](link to our 1-min intro video or audio). Furthermore, we make many friends, especially with members in TuDelft and SZU-China. We sincerely hope that our collaboration will extend to the next competition season. See you next summer! 亚欧交流会两张图片,第一张突出交流会,第二张突出参与感。都放,加注,链接去参与人,最好对方的也有,其他照片的说明类似
Heading east, \[iAPC](link to the event)
Between July 30th and August 2nd, we attended \[the 6th iGEM Asia Pacific Conference](link to the event). iGEMers from China, America, Japan and other countries gathered together to communicate about the progress of their projects. We have faced larger audiences than EuroAsian, and we were better prepared and made sure to create [a deep and thorough description] of our project. Participating in iAPC has not only offered us another excellent chance to practice our presentation skills but also given us additional helpful feedback regarding our experimental design and modeling.
As we traveled to Taiwan, we really took the opportunity to enjoy their nightlife and food, yummy. 照片玩的和吃的
Local + global, \[CCiC](link to the event)
Between August 28th and 31st, we participated in \[the 5th Conference of China iGEMer Community](link to the event) held in ShanghaiTech University, Shanghai. It was in the local, and some of us were participating in the conference during the day and working in the lab at night. It was a global event, 多少队伍多少国家.
During the conference, apart from presenting our ENABLE project and explaining our poster, we also distributed \[questionnaires regarding the explanation of logic gates](link to HP中相关文字,用 a # label) to collect opinions from our colleagues to improve the project. Moreover, we also had a valuable opportunity to meet the iGEM competition organizers Meagan face to face and ask them relevant questions regarding recent competition policy changes and the future of iGEM, etc. Overall, this conference has undoubtedly inspired us with new ideas and new methods, and has provided us with terrific feedbacks for improving our project!
Now, we are ready to head West. See you all in Boston, this coming winter. //各合作队伍的队徽 //our project 不够明确:默认每段落第一次出现用 our ENABLE project //思考是 我们是队伍 ENABLE是项目,合在一起 我们的ENABLE项目
Abstract
Contact-dependent signaling is critical for multicellular biological events, yet customizing contact-dependent signal transduction between cells remains challenging. Here we have developed the ENABLE toolbox, a complete set of transmembrane binary logic gates. Each gate consists of 3 layers: Receptor, Amplifier, and Combiner. We first optimized synthetic Notch receptors to enable cells to respond to different signals across the membrane reliably. These signals, individually amplified intracellularly by transcription, are further combined for computing. Our engineered zinc finger-based transcription factors perform binary computation and output designed products. In summary, we have combined spatially different signals in mammalian cells, and revealed new potentials for biological oscillators, tissue engineering, cancer treatments, bio-computing, etc. ENABLE is a toolbox for constructing contact-dependent signaling networks in mammals. The 3-layer design principle underlying ENABLE empowers any future development of transmembrane logic circuits, thus contributes a foundational advance to Synthetic Biology.